In silico and in vitro Study of the Inhibitory Effect of Antiinflammatory Drug Betamethasone on Two Lipases

Author(s): Nia Samira*, Benarous Khedidja, Abdelalim Fatima Zahra, Chellali Khadidja Nour Elyakine, Yousfi Mohamed

Journal Name: Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry
Formerly Current Medicinal Chemistry - Anti-Inflammatory & Anti-Allergy Agents

Volume 19 , Issue 4 , 2020


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Graphical Abstract:


Abstract:

Background: For the first time, the anti-inflammatory drug betamethasone is investigated for its inhibitory activity against lipase.

Objective: This work aims to demonstrate the in vitro and in silico inhibitory effect of the anti-inflammatory drug betamethasone on the enzymatic activity of two lipases.

Methods: In vitro study using p-nitrophenyllaurate as lipase substrate is used to determine inhibition potency. Molecular Docking is performed using the Autodock Vina for drug molecule and two enzymes Candida rugosa lipase and human pancreatic lipase.

Results: Betamethasone represents a moderate inhibition effect with a value of IC50 of 0.36±0.01 mg/ml. Molecular docking allowed us to understand inhibitory – enzyme interactions and to confirm in vitro obtained results.

Conclusion: These experiments showed that betamethasone can be used in the treatment of diseases related to lipase activity.

Keywords: Anti-inflammatory drugs, autodock vina, betamethasone, Candida rugosa, human pancreatic lipase, diseases related to lipase, inhibition, lipase, molecular docking.

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Article Details

VOLUME: 19
ISSUE: 4
Year: 2020
Published on: 06 September, 2019
Page: [387 - 392]
Pages: 6
DOI: 10.2174/1871523018666190906165944

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