Background: For the first time, the anti-inflammatory drug betamethasone is
investigated for its inhibitory activity against lipase.
Objective: This work aims to demonstrate the in vitro and in silico inhibitory effect of the
anti-inflammatory drug betamethasone on the enzymatic activity of two lipases.
Methods: In vitro study using p-nitrophenyllaurate as lipase substrate is used to determine
inhibition potency. Molecular Docking is performed using the Autodock Vina for drug
molecule and two enzymes Candida rugosa lipase and human pancreatic lipase.
Results: Betamethasone represents a moderate inhibition effect with a value of IC50 of
0.36±0.01 mg/ml. Molecular docking allowed us to understand inhibitory – enzyme interactions
and to confirm in vitro obtained results.
Conclusion: These experiments showed that betamethasone can be used in the treatment of
diseases related to lipase activity.
Keywords: Anti-inflammatory drugs, autodock vina, betamethasone, Candida rugosa, human pancreatic
lipase, diseases related to lipase, inhibition, lipase, molecular docking.
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