Green Synthesis of Silver Nanocomposites of Nigella sativa Seeds Extract for Hepatocellular Carcinoma

Author(s): Afreen Usmani, Anuradha Mishra*, Asif Jafri, Md Arshad, Mohd Aftab Siddiqui

Journal Name: Current Nanomaterials

Volume 4 , Issue 3 , 2019

Become EABM
Become Reviewer
Call for Editor

Graphical Abstract:


Background: Silver nanoparticles play a significant role in bioavailability and refining the compatibility of natural drugs in the treatment of various chronic diseases including different types of cancer.

Objective: Green synthesis of silver nanocomposites of Nigella sativa seeds extract to evaluate the anticancer effects against hepatocellular carcinoma using HepG2 cell lines.

Methods: The AgNCs were developed by treating aqueous extract of N. sativa seeds treated with silver nitrate (1mM) solution and were used to test its efficacy against hepatocellular carcinoma using HepG2 cell lines.

Results and Discussion: The Surface Plasmon Resonance (SPR) of prepared AgNCs showed a peak at 432 nm via UV spectroscopy. The selected N. sativa AgNCs were characterized for polydispersity, surface charge and size and the results showed 0.215±0.093 polydispersity index (PDI), zeta potential 18.8±0.372 mV and size range 10-20 nm, respectively. The Fourier transform infrared spectroscopy (FTIR) also showed various peak of functional groups that are possibly involved in the reduction of silver ion and synthesized the N. sativa silver nanocomposites, respectively. N. sativa AgNCs showed 89.954% drug release while in the case of extract release, it was only 33.821% in 24 hrs. Further, in vitro studies of N. sativa AgNCs against hepatocellular carcinoma showed good cytotoxic effect p<0.05 with 7.16 µg/ml IC50 value.

Conclusion: Thus, the present results revealed that green synthesis of N. sativa AgNCs can be an alternative tool for clinical application in cancer therapy; however, there is a need to find the mechanism and role of AgNCs inside the individual.

Keywords: Nigella sativa, silver nanoparticles, bioavailability, anticancer, apoptosis, nanocomposites.

WHO. Cancer 2018.
Usmani A, Mishra A, Ahmad M. Nanomedicines: a theranostic approach for hepatocellular carcinoma. Artif Cells Nanomed Biotechnol 2018; 46(4): 680-90.
[] [PMID: 28884605]
Harshita , Barkat MA, Rizwanullah M, et al. Paclitaxel-loaded nanolipidic carriers with improved oral bioavailability and anticancer activity against human liver carcinoma. AAPS PharmSciTech 2019; 20(2): 87.
[] [PMID: 30675689]
Moteriya P, Chanda S. Synthesis and characterization of silver nanoparticles using Caesalpinia pulcherrima flower extract and assessment of their in vitro antimicrobial, antioxidant, cytotoxic, and genotoxic activities. Artif Cells Nanomed Biotechnol 2017; 45(8): 1556-67.
[] [PMID: 27900878]
Watkins R, Wu L, Zhang C, Davis RM, Xu B. Natural product-based nanomedicine: recent advances and issues. Int J Nanomedicine 2015; 10: 6055-74.
[PMID: 26451111]
Díaz MR, Vivas-Mejia PE. Nanoparticles as drug delivery systems in cancer medicine: emphasis on RNAi-containing nanoliposomes. Pharmaceuticals 2013; 6(11): 1361-80.
[] [PMID: 24287462]
Thapa RK, Khan GM, Parajuli-baral K, Thapa P. Herbal medicine incorporated nanoparticles: advancements in herbal treatment. Asian J Biomed Pharm 2013; 03(24): 7-14.
Usmani A, Mishra A. Metallic nanoformulations : green synthetic approach for advanced drug delivery metallic nanoformulations green synthetic approach for advanced drug delivery. Mater Sci Adv Compos Mater 2018; 2(2): 1-5.
Amin M, Anwar F, Janjua MR, Iqbal MA, Rashid U. Green synthesis of silver nanoparticles through reduction with Solanum xanthocarpum L. berry extract: characterization, antimicrobial and urease inhibitory activities against Helicobacter pylori. Int J Mol Sci 2012; 13(8): 9923-41.
[] [PMID: 22949839]
Sinha SN, Paul D. Phytosynthesis of silver nanoparticles using Andrographis paniculata leaf extract and evaluation of their antibacterial activities. Spectrosc Lett 2015; 48(8): 600-4.
Khatami M, Pourseyedi S, Khatami M, Hamidi H, Zaeifi M, Soltani L. Synthesis of silver nanoparticles using seed exudates of Sinapis arvensis as a novel bioresource, and evaluation of their antifungal activity. Bioresour Bioprocess 2015; 2(1): 56-64.
Rao PV, Nallappan D, Madhavi K, Rahman S, Jun Wei L, Gan SH. Phytochemicals and biogenic metallic nanoparticles as anticancer agents. Oxid Med Cell Longev 2016.20163685671
[] [PMID: 27057273]
Daisy P, Saipriya K. Biochemical analysis of Cassia fistula aqueous extract and phytochemically synthesized gold nanoparticles as hypoglycemic treatment for diabetes mellitus. Int J Nanomedicine 2012; 7: 1189-202.
[] [PMID: 22419867]
Santhoshkumar T, Rahuman AA, Rajakumar G, et al. Synthesis of silver nanoparticles using Nelumbo nucifera leaf extract and its larvicidal activity against malaria and filariasis vectors. Parasitol Res 2011; 108(3): 693-702.
[] [PMID: 20978795]
Sukirtha R, Priyanka KM, Antony JJ, Kamalakkannan S, Thangam R, Gunasekaran P, et al. Cytotoxic effect of Green synthesized silver nanoparticles using Melia azedarach against in vitro HeLa cell lines and lymphoma mice model. Process Biochem 2012; 47(2): 273-9.
Alfuraydi AA, Devanesan S, Al-Ansari M, AlSalhi MS, Ranjitsingh AJ. Eco-friendly green synthesis of silver nanoparticles from the sesame oil cake and its potential anticancer and antimicrobial activities. J Photochem Photobiol B 2019; 192: 83-9.
[] [PMID: 30710829]
Venugopal K, Ahmad H, Manikandan E, et al. The impact of anticancer activity upon Beta vulgaris extract mediated biosynthesized silver nanoparticles (ag-NPs) against human breast (MCF-7), lung (A549) and pharynx (Hep-2) cancer cell lines. J Photochem Photobiol B 2017; 173: 99-107.
[] [PMID: 28570910]
Kumar B, Smita K, Seqqat R, Benalcazar K, Grijalva M, Cumbal L. In vitro evaluation of silver nanoparticles cytotoxicity on Hepatic cancer (Hep-G2) cell line and their antioxidant activity: Green approach for fabrication and application. J Photochem Photobiol B 2016; 159: 8-13.
[] [PMID: 27010841]
Rathi Sre PR, Reka M, Poovazhagi R, Arul Kumar M, Murugesan K. Antibacterial and cytotoxic effect of biologically synthesized silver nanoparticles using aqueous root extract of Erythrina indica lam. Spectrochim Acta-Part A Mol Biomol Spectrosc 2015; 135: 1137-44.
Arockia John Paul J, Karunai Selvi B, Karmegam N. Biosynthesis of silver nanoparticles from Premna serratifolia L. leaf and its anticancer activity in CCl4-induced hepato-cancerous Swiss albino mice. Appl Nanosci 2015; 5(8): 937-44.
Iravani S, Korbekandi H, Zolfaghari B. Synthesis of silver nanoparticles : chemical, physical and biological methods synthesis of silver NPs. Res Pharm Sci 2016; 9(6): 1-17.
Marbat MM, Ali M, Hadi AM. The use of Nigella sativa as a single agent in treatment of male infertility. Tikrit J Pharm Sci 2013; 9(1): 19-29.
Tembhurne SV, Feroz S, More BH, Sakarkar DM. A review on therapeutic potential of Nigella sativa (kalonji) seeds. J Med Plants Res 2014; 8(3): 167-77.
Islam MH, Ahmad IZ, Salman MT. Neuroprotective effects of Nigella sativa extracts during germination on central nervous system. Pharmacogn Mag 2015; 11(42)(Suppl. 1): S182-9.
[] [PMID: 26109765]
Aftab A, Yousaf Z. Nigella sativa l from traditional. Contemp Med 2018; pp. 20-8.
Majdalawieh AF, Fayyad MW. Recent advances on the anti-cancer properties of Nigella sativa, a widely used food additive. J Ayurveda Integr Med 2016; 7(3): 173-80.
[] [PMID: 27649635]
Gandhiraj V, Sathish Kumar K, Madhusudhanan J, Sandhya J. Antitumor activity of biosynthesized silver nano particles from leaves of Momordica charantia against MCF-7 cell line. Int J Chemtech Res 2015; 8(7): 351-62.
Samajdar S. Extraction and chemical tests on Nigella sativa l. collected from punjab region of India. Pharmatutor 2017; 5(12): 54.
Muthulakshmi A, Jothibai Margret R, Mohan VR. GC-MS analysis of bioactive components of Feronia elephantum (Rutaceae). J Appl Pharm Sci 2012; 2(2): 69-74.
Janakiraman N, Johnson M, Sahaya SS. GC-MS analysis of bioactive constituents of Peristrophe bicalyculata (Retz.) Nees. (Acanthaceae). Asian Pac J Trop Biomed 2012; 2(1)(Suppl.): S46-9.
Li Q, Zhang Z, Haque SS, Zhang M, Xia L. Localized surface plasmon resonance effects by naturally occurring Chinese yam particles. J Appl Phys 2010; 108(12): 48-54.
[] [PMID: 21267084]
Dhand V, Soumya L, Bharadwaj S, Chakra S, Bhatt D, Sreedhar B. Green synthesis of silver nanoparticles using Coffea arabica seed extract and its antibacterial activity. Mater Sci Eng C 2016; 58: 36-43.
[] [PMID: 26478284]
AboulFotouh K. Allam AA, El-Badry M, El-Sayed AM. Development and in vitro/in vivo performance of self-nanoemulsifying drug delivery systems loaded with candesartan cilexetil. Eur J Pharm Sci 2017; 109: 503-13.
[] [PMID: 28889028]
Ahmad M. Sahabjada, Akhtar J, et al. Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line. EXCLI J 2017; 16: 810-23.
[PMID: 28694767]
Ahamad MS, Siddiqui S, Jafri A, Ahmad S, Afzal M, Arshad M. Induction of apoptosis and antiproliferative activity of naringenin in human epidermoid carcinoma cell through ROS generation and cell cycle arrest. PLoS One 2014; 9(10)e110003
[] [PMID: 25330158]
Siddiqui S, Ahamad MS, Jafri A, Afzal M, Arshad M. Piperine triggers apoptosis of human oral squamous carcinoma through cell cycle arrest and mitochondrial oxidative stress. Nutr Cancer 2017; 69(5): 791-9.
[] [PMID: 28426244]
Siddiqui S, Ahmad E, Gupta M, et al. Cissus quadrangularis Linn exerts dose-dependent biphasic effects: osteogenic and anti-proliferative, through modulating ROS, cell cycle and Runx2 gene expression in primary rat osteoblasts. Cell Prolif 2015; 48(4): 443-54.
[] [PMID: 26079044]
Jha AK, Prasad K, Prasad K, Kulkarni AR. Plant system: nature’s nanofactory. Colloids Surf B Biointerfaces 2009; 73(2): 219-23.
[] [PMID: 19539452]
Powers KW, Brown SC, Krishna VB, Wasdo SC, Moudgil BM, Roberts SM. Research strategies for safety evaluation of nanomaterials. Part VI. Characterization of nanoscale particles for toxicological evaluation. Toxicol Sci 2006; 90(2): 296-303.
[] [PMID: 16407094]
Parmar N, Singla N, Amin S, Kohli K. Study of cosurfactant effect on nanoemulsifying area and development of lercanidipine loaded (SNEDDS) self nanoemulsifying drug delivery system. Colloids Surf B Biointerfaces 2011; 86(2): 327-38.
[] [PMID: 21550214]
Netala VR, Kotakadi VS, Domdi L, et al. Biogenic silver nanoparticles: efficient and effective antifungal agents. Appl Nanosci 2016; 6(4): 475-84.
Kotakadi VS, Gaddam SA, Subba Rao Y, Prasad TNVKV, Varada Reddy A, Sai Gopal DVR. Biofabrication of silver nanoparticles using Andrographis paniculata. Eur J Med Chem 2014; 73: 135-40.
[] [PMID: 24389508]
Reddy YP, Chandrasekhar KB, Sadiq MJ. A study of Nigella sativa induced growth inhibition of MCF and HepG2 cell lines: An anti-neoplastic study along with its mechanism of action. Pharmacognosy Res 2015; 7(2): 193-7.
[] [PMID: 25829794]

open access plus

Rights & PermissionsPrintExport Cite as

Article Details

Year: 2019
Published on: 11 November, 2019
Page: [191 - 200]
Pages: 10
DOI: 10.2174/2468187309666190906130115

Article Metrics

PDF: 22