Background: Osteoarthritis (OA) pertains to a chronic disease of degenerative joints distinguished by
articular cartilage destruction, subchondral bone remodeling, osteophyte formation, and inflammatory changes.
Chondrocyte apoptosis is inextricably linked to cartilage degeneration. SRY-related high-mobility-group-box 9
(SOX9) is a well-acknowledged transcription factor in the chondrogenesis. Nevertheless, the detailed function of
miR-138-5p/SOX9 in OA remains to be fully clarified.
Materials and Methods: qRT-PCR was performed to measure the expressions of miR-138-5p and SOX9 mRNA
in OA and normal cartilage tissues and cells. Human chondrocyte cell lines, CHON-001 and ATDC5, were
treated with different doses of interleukin-1β (IL-1β) to simulate the inflammatory response environment of OA.
miR-138-5p mimics, miR-138-5p inhibitors, and SOX9 small interfering RNA (siRNA) were constructed and
transfected into CHON-001 and ATDC5 cells. CCK-8 was conducted to determine the cell viability and transwell
assay was used to monitor the migration of cells. Western blot was carried out to detect the expressions of apoptosis-
related factors. Enzyme-linked immunosorbent assay (ELISA) was adopted to measure the contents of inflammatory
factors. TargetScan predicted SOX9 was a target gene of miR-138-5p, which was then verified by
Results: miR-138-5p expression was down-regulated in OA and regulated SOX9 expression. The downregulation
of miR-138-5p facilitated the proliferation and migration of CHON-001 and ATDC5 cells, while impeded
their apoptosis and inflammatory response. Besides, down-regulated SOX9 can counteract the promoting
effect of down-regulated miR-138-5p on the proliferation and migration of chondrocytes.
Conclusion: miR-138-5p can arrest the proliferation and migration of CHON-001 and ATDC5 via restraining
SOX9, and facilitate the apoptosis and inflammation. This study revealed the protective effect of down-regulated
miR-138-5p on the inflammatory injury of chondrocytes caused by IL-1β.