Title:Evaluation of Anticancer Activities of Gallic Acid and Tartaric Acid Vectorized on Iron Oxide Nanoparticles
VOLUME: 10 ISSUE: 2
Author(s):Lina Saleh, Eman A. Ragab, Heba K. Abdelhakim, Sabrein H. Mohamed and Zainab Zakaria*
Affiliation:Biochemistry Department, Cairo University, Giza, Organic Chemistry Department, Faculty of Science, Cairo University, Giza, Biochemistry Department, Chemistry Department, Cairo University, Giza, Chemistry Department, Faculty of Science, Cairo University, Giza, Biomedical Research Lab, Faculty of Pharmacy, Heliopolis University for Sustainable Development, Cairo
Keywords:Super paramagnetic nanoparticle, gallic acid, bee venom, tartaric acid, cytotoxicity, gene expression.
Abstract:Background: Cancer is one of the leading causes of death. New tactics targeting the survival
pathways that provide effective drugs are being developed.
Objective: Super paramagnetic nanoparticle serves as drug carrier for drug delivery system. Herein,
Iron oxide-CMC-TA and Iron oxide-CMC-GA nanoparticles are synthesized for this target.
Methods: Iron oxide (Fe2O3) nanoparticles are synthesized, bound to carboxymethyl chitosan (CMC)
which are then conjugated to tartaric acid (TA) or gallic acid (GA) to form Iron oxide-CMC-TA and
Iron oxide-CMC-GA nanoparticles. Those nanoparticles were characterized and the cytotoxicity effect
was evaluated when associated with/without bee venom to measure the synergistic effect on A549 and
WI-38 cell lines. In addition, apoptotic genes expression in A549 was evaluated when treated with both
nanoparticles.
Results: We showed that the cytotoxicity effect of TA and GA on A549 and WI-38 cell lines was increased
when they immobilized on iron oxide-CMC nanoparticles and the effect was synergistically
elevated when added to bee venom. The cytotoxic activity of these two nanoparticles was higher in
A549 cancer cell line when compared with WI-38 normal cell line. Moreover, the expression of apoptotic
genes was elevated.
Conclusion: Iron oxide-CMC-TA nanoparticle and Iron oxide-CMC-GA nanoparticle can selectively
induce apoptosis in cancer cell lines more than in normal cell lines, which is an important aspect in cancer
cell targeting process to minimize damage upon normal cells.
