Dose-response Evaluation of Propolis Dental Varnish in Children: A Randomized Control Study

Author(s): Edilson Martins Rodrigues Neto, Lídia Audrey Rocha Valadas*, Patrícia Leal Dantas Lobo, Analice Mendes Barreto Fernandes, Said Gonçalves da Cruz Fonseca, Francisco Vagnaldo Fechine, Francisco Josimar Girão Júnior, Mary Anne Medeiros Bandeira, Marta Maria de França Fonteles

Journal Name: Recent Patents on Biotechnology

Volume 14 , Issue 1 , 2020

Become EABM
Become Reviewer

Graphical Abstract:


Abstract:

Background: Early childhood caries is a sugar-dependent disease with multifactorial modulating factors affecting deciduous dentition. It is defined as the presence of at least one decayed tooth, absence of a tooth due to caries or the existence of a temporary restoration in a tooth in a child between zero and 71 months of age. No BRP varnish was found in intellectual property banks, therefore it was registered and deposited with patent number BR1020160190142.

Objective: The objective of this study was to evaluate the dose-response concentration of alcoholic extract of Brazilian red propolis (BRP), in the form of dental varnish, against Streptococcus mutans (S. mutans) in children.

Methods: Twenty-four children, aged between 36 and 71 months, of both genders and without caries, were selected to participate in this pilot study and grouped randomly into four groups to receive different concentrations of BRP varnish (1%, 2.5%, 5% and 10%). The varnish was applied to the surface of all second deciduous molars. The antimicrobial activity was observed in saliva, which was collected in two phases: before applying the BRP varnish and after use.

Results: There was microbiological reduction of S. mutans in the oral cavity of the children in all the tested concentrations. The highest percentage reduction of S. mutans was observed at the concentration of 2.5% (P = 0.0443).

Conclusion: The BRP extract in the form of dental varnish has antimicrobial activity against S. mutans and constitutes a possible alternative in the prevention of dental caries.

Keywords: Propolis, saliva, Streptococcus mutans, dentistry, BRP varnish, BRP extract.

[1]
American Academy of Pediatric Dentistry. Policy on early childhood caries (ECC): classifications, consequences, and preventive strategies. Pediatr Dent 2005-2006; 27(7): 31-3.
[PMID: 16541878]
[2]
Pitts NB, Zero DT, Marsh PD, Ekstrand K, Weintraub JA, Ramos-Gomez F, et al. Dental caries. Nat Rev Dis Primers 2017; 3: 17030.
[http://dx.doi.org/10.1038/nrdp.2017.30] [PMID: 28540937 ]
[3]
Valadas LAR, Gurgel MF, Mororó JM, Fonseca SGD-C, Fonteles CSR, de Carvalho CBM, et al. Dose-response evaluation of a copaiba-containing varnish against Streptococcus mutans in vivo. Saudi Pharm J 2019; 27(3): 363-7.
[4]
De-Carli AD, Zárate-Pereira P, De-Carli G, Zafalon EJ, de Rezende Zárate CB, Yassumoto LM. Ação da própolis de apis mellifera associada ao fluoreto de sódio sobre o biofilme dental: ensaio clínico duplo cego randomizado (2011). Revista Odontológica do Brasil Central 2011; 19(51): 310-3.
[5]
Xiang SW, Shao J, He J, Wu XY, Xu XH, Zhao WH. A Membrane-targeted peptide inhibiting PtxA of phosphotransferase system blocks Streptococcus mutans. Caries Res 2019; 53(2): 176-93.
[http://dx.doi.org/10.1159/000489607] [PMID: 30107375]
[6]
Kaur R, Gilbert SC, Sheehy EC, Beighton D. Salivary levels of Bifidobacteria in caries-free and caries-active children. Int J Paediatr Dent 2013; 23(1): 32-8.
[http://dx.doi.org/10.1111/j.1365-263X.2011.01220.x] [PMID: 22309226]
[7]
Eriksson L, Lif Holgerson P, Johansson I. Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community. Sci Rep 2017; 7(1): 5861.
[http://dx.doi.org/10.1038/s41598-017-06221-z] [PMID: 28724921]
[8]
Lobo PLD, Filho HGL, Filho JGL. Desenvolvimento de verniz dentário de própolis vermelha para controle de cárie dentária BR1020160190142. 2016.
[9]
Moura SAB, Medeiros AMC, Costa FRH, Moraes PH, Filho SAO. Diagnostic value of saliva in oral and systemic diseases: a literature review. Pesq Bras Odontoped Clin Integr 2008; 7(2): 187-94.
[http://dx.doi.org/10.4034/1519.0501.2007.0072.0014]
[10]
Lins R, Vasconcelos FHP, Leite RB, Coelho-Soares RS, Barbosa DN. Avaliação clínica de bochechos com extratos de Aroeira (Schinus terebinthifolius) e Camomila (Matricaria recutita L.) sobre a placa bacteriana e a gengivite. Rev Bras Pl Med 2013; 15(1): 112-20.
[http://dx.doi.org/10.1590/S1516-05722013000100016]
[11]
Borba AM, Macedo M, Walter LRF. Alternative dentistry with medicinal plants in Chapada dos Guimarães – Mato Grosso – Brazil. RSBO 2008; 5(1): 43-50.
[12]
Lobo PL, Fonteles CS, de Carvalho CB, do Nascimento DF, da Cruz Fonseca SG, Jamacaru FV, et al. Dose-response evaluation of a novel essential oil against mutans streptococci in vivo. Phytomedicine 2011; 18(7): 551-6.
[http://dx.doi.org/10.1016/j.phymed.2010.10.018] [PMID: 21112195]
[13]
Lobo PL, Fonteles CS, Marques LA, Jamacaru FV, Fonseca SG, de Carvalho CB, et al. The efficacy of three formulations of Lippia sidoides Cham. Essential oil in the reduction of salivary Streptococcus mutans in children with caries: a randomized, double-blind, controlled study. Phytomedicine 2014; 21(8-9): 1043-7.
[http://dx.doi.org/10.1016/j.phymed.2014.04.021] [PMID: 24863037]
[14]
Anauate NC, Marcucci MC, Paulino N, Anido-Anido A, Amore R, de Mendonça S, et al. Effects of typified propolis on mutans streptococci and lactobacilli: a randomized clinical trial. Braz Dent Sci 2013; 16(2): 31-6.
[http://dx.doi.org/10.14295/bds.2013.v16i2.879] [PMID: 24494174]
[15]
Lustosa SR, Galindo AB, Nunes LC, Randau KP, Rolim Neto PJ. Propolis: updates on chemistry and pharmacology. Rev Bras Farmacogn 2008; 18(3): 447-54.
[http://dx.doi.org/10.1590/S0102-695X2008000300020]
[16]
Longhini R, Raksa SM, Oliveira ACP, Svidzinski TIE, Franco SL. Obtenção de extratos de própolis sob diferentes condições e avaliação de sua atividade antifúngica. Rev Bras Farmacogn 2007; 17: 388-95.
[http://dx.doi.org/10.1590/S0102-695X2007000300015]
[17]
Libério SA, Pereira AL, Araújo MJ, Dutra RP, Nascimento FR, Monteiro-Neto V, et al. The potential use of propolis as a cariostatic agent and its actions on mutans group streptococci. J Ethnopharmacol 2009; 125(1): 1-9.
[http://dx.doi.org/10.1016/j.jep.2009.04.047] [PMID: 19422903]
[18]
Freires IA, de Alencar SM, Rosalen PL. A pharmacological perspective on the use of Brazilian red propolis and its isolated compounds against human diseases. Eur J Med Chem 2016; 110: 267-79.
[http://dx.doi.org/10.1016/j.ejmech.2016.01.033] [PMID: 26840367]
[19]
Bueno-Silva B, Koo H, Falsetta ML, Alencar SM, Ikegaki M, Rosalen PL. Effect of neovestitol-vestitol containing Brazilian red propolis on accumulation of biofilm in vitro and development of dental caries in vivo. Biofouling 2013; 29(10): 1233-42.
[http://dx.doi.org/10.1080/08927014.2013.834050] [PMID: 24099330]
[20]
do Nascimento TG, da Silva PF, Azevedo LF, et al. Polymeric nanoparticles of brazilian red propolis extract: preparation, characterization, antioxidant and leishmanicidal activity. Nanoscale Res Lett 2016; 11(1): 301.
[21]
Celerino de Moraes Porto IC, Chaves Cardoso de Almeida D, et al. Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent. BMC Complement Altern Med 2018; 18(1): 219.
[http://dx.doi.org/10.1186/s12906-018-2281-y] [PMID: 30021632]
[22]
DE Luca MP, Freires IA, Gala-García A, Santos VR, Vale MP, Alencar SM, et al. The anti-caries activity and toxicity of an experimental propolis-containing varnish. Braz Oral Res 2017; 31 e45
[http://dx.doi.org/10.1590/1807-3107bor-2017.vol31.0045] [PMID: 28591241]
[23]
Franca JR, Luca MP, Ribeiro TG, Castilho RO, Moreira AN, Santos VR, et al. Propolis-based chitosan varnish: drug delivery, controlled release and antimicrobial activity against oral pathogen bacteria. BMC Complement Altern Med 2014; 14: 478.
[http://dx.doi.org/10.1186/1472-6882-14-478] [PMID: 25495921]
[24]
Furtado JHC Junior, Marques LARV, Neto EMR, Fonteles MM. Desenvolvimento de dentifrícios incorporados com própolis vermelha brasileira associados a arginina,flúor e hidroxiapatita para controle de microorganismos orais BR1020170110974. 2018.
[25]
Franco AAG, Franca JR, Santos VR, De Luca MP, Moreira NA. Verniz polimérico de própolis PI1005619-0. 2013.
[26]
Fonseca SGC, Neto EMR, Fonteles MMDF, Barros KBNT, Santos SLF. Desenvolvimento de gel incorporados com própolis vermelha brasileira para controle de microrganismos dérmicos faciais BR1020170212 297. 2018.
[27]
Tanikawa-Vergilio KL, Santos EM, Guedes CC, Motta LJ, Bussadori SK. Avaliação in vitro do potencial antimicrobiano de cinco tipos diferentes de vernizes de flúor e clorexidina. ConScientiae Saúde 2008; 7(1): 55-60.
[http://dx.doi.org/10.5585/conssaude.v7i1.747]
[28]
American Academy of Pediatric Dentistry (AAPD) Clinical Practice Guidelines 2014; 37: 132-9.
[29]
Carvalho DM, Salazar M, Oliveira BHD, Coutinho ESF. Fluoride varnishes and decrease in caries incidence in preschool children: a systematic review. Rev Bras Epidemiol 2010; 13(1): 139-49.
[http://dx.doi.org/10.1590/S1415-790X2010000100013] [PMID: 20683562]
[30]
Weyant RJ, Tracy SL, Anselmo TT, Beltrán-Aguilar ED, Donly KJ, Frese WA, et al. Topical fluoride for caries prevention: executive summary of the updated clinical recommendations and supporting systematic review. J Am Dent Assoc 2013; 144(11): 1279-91.
[http://dx.doi.org/10.14219/jada.archive.2013.0057] [PMID: 24177407]
[31]
Furtado Jr JH, Valadas LAR, Mendonça KS, de Oliveira Filho RD, Gadelha LMU, Fiallos NM. Products of dental use containing copaiba oil-resin: technological prospecting based on patents. Recent Pat Biotechnol 2018; 12(4): 288-96.
[http://dx.doi.org/10.2174/2211550107666180815114855] [PMID: 30156167]
[32]
Duarte S, Rosalen PL, Hayacibara MF, Cury JA, Bowen WH, Marquis RE, et al. The influence of a novel propolis on mutans streptococci biofilms and caries development in rats. Arch Oral Biol 2006; 51(1): 15-22.
[http://dx.doi.org/10.1016/j.archoralbio.2005.06.002] [PMID: 16054589]
[33]
Nogueira MA, Diaz MG, Tagami PM, Lorscheide J. Atividade microbiana de óleos essenciais e extratos de própolis sobre bactérias cariogênicas. Rev Bras Cienc Farm 2007; 28(1): 93-7.


Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 14
ISSUE: 1
Year: 2020
Page: [41 - 48]
Pages: 8
DOI: 10.2174/1872208313666190826145453
Price: $65

Article Metrics

PDF: 17
HTML: 3