Background: Early childhood caries is a sugar-dependent disease with multifactorial
modulating factors affecting deciduous dentition. It is defined as the presence of at least one decayed
tooth, absence of a tooth due to caries or the existence of a temporary restoration in a tooth
in a child between zero and 71 months of age. No BRP varnish was found in intellectual property
banks, therefore it was registered and deposited with patent number BR1020160190142.
Objective: The objective of this study was to evaluate the dose-response concentration of alcoholic
extract of Brazilian red propolis (BRP), in the form of dental varnish, against Streptococcus mutans
(S. mutans) in children.
Methods: Twenty-four children, aged between 36 and 71 months, of both genders and without
caries, were selected to participate in this pilot study and grouped randomly into four groups to receive
different concentrations of BRP varnish (1%, 2.5%, 5% and 10%). The varnish was applied
to the surface of all second deciduous molars. The antimicrobial activity was observed in saliva,
which was collected in two phases: before applying the BRP varnish and after use.
Results: There was microbiological reduction of S. mutans in the oral cavity of the children in all
the tested concentrations. The highest percentage reduction of S. mutans was observed at the concentration
of 2.5% (P = 0.0443).
Conclusion: The BRP extract in the form of dental varnish has antimicrobial activity against
S. mutans and constitutes a possible alternative in the prevention of dental caries.