Title:Recent Patents on Impact of Lipopeptide on the Biofilm Formation onto Titanium and Stainless Steel Surfaces
VOLUME: 14 ISSUE: 1
Author(s):Mauro Ezio Eustáquio Pires, Adriano Guimarães Parreira, Tuânia Natacha Lopes Silva, Heloísa Carneiro Colares, José Antonio da Silva, Juliana Teixeira de Magalhães, Alexsandro Sobreira Galdino, Daniel Bonoto Gonçalves, José Mauro Granjeiro* and Paulo Afonso Granjeiro*
Affiliation:Biotechnology Process and Macromolecules Purification Laboratory, Campus Centro Oeste, Federal University of Sao Joao Del-Rei, Divinopolis, MG, Zip Code: 35501296, Biotechnology Process and Macromolecules Purification Laboratory, Campus Centro Oeste, Federal University of Sao Joao Del-Rei, Divinopolis, MG, Zip Code: 35501296, Biotechnology Process and Macromolecules Purification Laboratory, Campus Centro Oeste, Federal University of Sao Joao Del-Rei, Divinopolis, MG, Zip Code: 35501296, Biotechnology Process and Macromolecules Purification Laboratory, Campus Centro Oeste, Federal University of Sao Joao Del-Rei, Divinopolis, MG, Zip Code: 35501296, Biotechnology Process and Macromolecules Purification Laboratory, Campus Centro Oeste, Federal University of Sao Joao Del-Rei, Divinopolis, MG, Zip Code: 35501296, Microbiology Laboratory, Campus Centro Oeste, Federal University of Sao Joao Del-Rei, Divinópolis, MG, 35501296, Microbial Biotechnology Laboratory, Campus Centro Oeste, Federal University of Sao Joao Del-Rei, Divinopolis, MG, 35501296, Biotechnology Process and Macromolecules Purification Laboratory, Campus Centro Oeste, Federal University of Sao Joao Del-Rei, Divinopolis, MG, Zip Code: 35501296, Bioengineering Laboratory, National Institute of Metrology, Quality and Technology, Xerem, Duque de Caxias, RJ, 25250-020, Biotechnology Process and Macromolecules Purification Laboratory, Campus Centro Oeste, Federal University of Sao Joao Del-Rei, Divinopolis, MG, Zip Code: 35501296
Keywords:Lipopeptide, Bacillus subtilis, titanium, stainless steel, biofilm, arthroplasties.
Abstract:
Background: Numerous causes of infection in arthroplasties are related to biofilm formation
on implant surfaces. In order to circumvent this problem, new alternatives to prevent bacterial
adhesion biosurfactants-based are emerging due to low toxicity, biodegradability and antimicrobial
activity of several biosurfactants. We revised all patents relating to biosurfactants of applicability
in orthopedic implants.
Methods: This work aims to evaluate the capability of a lipopeptide produced by Bacillus subtilis
ATCC 19659 isolates acting as inhibitors of the adhesion of Escherichia coli ATCC 25922 and
Staphylococcus aureus ATCC 29213 onto titanium and stainless steel surfaces and its antimicrobial
activity.
Results: The adhesion of the strains to the stainless-steel surface was higher than that of titanium.
Preconditioning of titanium and stainless-steel surfaces with 10 mg mL-1 lipopeptide reduced the
adhesion of E. coli by up to 93% and the adhesion of S. aureus by up to 99.9%, suggesting the
strong potential of lipopeptides in the control of orthopedic infections. The minimal inhibitory
concentration and minimum bactericidal concentration were 10 and 240 µg mL-1 for E. coli and
S. aureus, respectively.
Conclusion: The lipopeptide produced by Bacillus subtilis ATCC 19659 presented high biotechnological
application in human health against orthopedic implants infections.
