Biochemical Implications of Biotransformation of Some Toxic Floras Using Natural Local Enzyme Sources

Author(s): Emmanuel N. Agomuo, Peter U. Amadi*.

Journal Name: Recent Patents on Biotechnology

Volume 14 , Issue 1 , 2020

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Abstract:

Background: Recently, it has been established that simultaneous saccharification and fermentation is a potent technique for the detoxification of harmful plant materials.

Objective: Following encouraging simultaneous medicinal applications of snail slime and yeast, we exploited their hydrolytic and fermentation potentials to prevent toxicities of the selected floras; Erythrodontium barteri (EB), bracken fern (BF), and crustose lichens (CL). The applicability of the saccharification process has been described in a patent (WO2005010193A2).

Methods: The plants were bioprocessed using snail digestive juice and yeast slurry and their health effects were evaluated. Seventy rats were divided equally into groups, treated with single doses of aqueous extracts of the plants and their bioprocessed forms, and compared with control rats.

Results: The plants showed very high antinutrients levels, which significantly reduced after SSF with enhanced flavonoids, alkaloids and phenols. Potential alterations of WBC differentials, RBC, liver and renal function markers indices were mitigated by bioprocessed extracts. MDA, SOD, GRase, XO and XDH levels in rats administered the bEB and CL were equivalent to the levels found for the control rats. Some bioprocessed plants produced unaltered insulin, ghrelin, and leptin levels. The bioprocessed extracts, when compared to the effects of unprocessed extracts, produced lower TNF-α, Caspase-3, and adiponectin levels and mitigated the potential suppression of Na+/K+-ATPase levels. Potential depletion of inhibin-B, testosterone, estrogen, and prolactin was mitigated after bioprocessing.

Conclusion: This study, thus, validates the application of bioprocessing using snail digestive juice and yeast as an effective approach to reduce the potential toxicities of harmful plants.

Keywords: Biotransformation, organ toxicity, fermentation, hormones, Na+/K+-ATPase, enzyme.

[1]
Amadi PU, Agomuo EN, Bob-Chile AA, Njoku UC, Ifeanacho MO, Okereke JC, et al. Toxicities selected medicinal plants, and floras of lower phyla. Alexandria Med J 2018; 54(4): 587-96.
[http://dx.doi.org/10.1016/j.ajme.2018.05.001]
[2]
Onianwa PC. Trace metal levels in some Nigerian mosses used as indicators of atmospheric pollution. Environ Pollut 1983; 5: 71-81.
[http://dx.doi.org/10.1016/0143-148X(83)90051-4]
[3]
Babarinde NAA, Oyesiku OO, Omolola FD. Isotherm and thermodynamic studies of the biosorption of copper (II) ions by Erythrodontium barteri. Int J Phys Sci 2007; 2(11): 300-4.
[4]
Wolff G, Pereira GC, Castro EM, Louzada J, Coelho FF. The use of Salvinia auriculata as a bioindicator in aquatic ecosystems: biomass and structure dependent on the cadmium concentration. Braz J Biol 2012; 72(1): 71-7.
[http://dx.doi.org/10.1590/S1519-69842012000100009] [PMID: 22437387]
[5]
Zuquim G, Tuomisto H, Jones MM, Prado J, Figueiredo FOG, Moulatle GM, et al. Predicting environmental gradients with fern species composition in Brazilian Amazonia. J Veg Sci 2014; 25(5): 1195-207.
[6]
Kubicka K, Samecka-Cymerman A, Kolon K, Kosiba P, Kempers AJ. Chromium and nickel in Pteridium aquilinum from environments with various levels of these metals. Environ Sci Pollut Res Int 2015; 22(1): 527-34.
[http://dx.doi.org/10.1007/s11356-014-3379-5] [PMID: 25087499]
[7]
Norton S. Toxic effects of plants. In: Klaassen CD, Ed. Cassarett and Doull’s toxicology, basic science of poison. 7th ed. United States: McGraw Hill 2008; pp. 1103-4.
[8]
Lucena RB, Rissi DR, Kommers GD, Pierezan F, Oliveira-Filho JC, Macêdo JT, et al. A retrospective study of 586 tumours in Brazilian cattle. J Comp Pathol 2011; 145(1): 20-4.
[http://dx.doi.org/10.1016/j.jcpa.2010.11.002] [PMID: 21247583]
[9]
Di Loria A, Piantedosi D, Cortese L, Roperto S, Urraro C, Paciello O, et al. Clotting profile in cattle showing chronic enzootic haematuria (CEH) and bladder neoplasms. Res Vet Sci 2012; 93(1): 331-5.
[10]
Huang X, Choi Y, Im H, Yarimaga O, Yoon E, Kim H. Aspartate aminotransferase (AST/GOT) and alanine aminotransferase (ALT/GPT) detection techniques. Sensors 2006; 6(7): 756-82.
[http://dx.doi.org/10.3390/s6070756]
[11]
Shahin M, Moore MR, Worrall S, Smith BL, Seawright AA, Prakash AS. H-Ras activation is an early event in the ptaquiloside-induced carcinogenesis: comparison of acute and chronic toxicity in rats. Biochem Biophys Res Commun 1998; 250(2): 491-7.
[12]
Lee RE. Phycology. 4th ed. Cambridge: Cambridge University Press 2008. ISBN 9780521682770
[http://dx.doi.org/10.1017/CBO9780511812897]
[13]
Conti ME, Cecchetti G. Biological monitoring: lichens as bioindicators of air pollution assessment-a review. Environ Pollut 2001; 114(3): 471-92.
[http://dx.doi.org/10.1016/S0269-7491(00)00224-4] [PMID: 11584645]
[14]
Syeda SF, Safina N, Hamayun S, Muhammad E. Lichens as bioindicators of air pollution from vehicular emissions in district Poonch, Azad jammu and Kashmir, Pakistan. Pak J Bot 2017; 49(5): 1801-10.
[15]
Reddy NR, Pierson MD. Reduction in antinutritional and toxic components in plant foods by fermentation. Food Res Int 1994; 27(3): 281-90.
[http://dx.doi.org/10.1016/0963-9969(94)90096-5]
[16]
Adeyemo SM, Onilude AA. Reduction of anti-nutritional factors in fermenting soybeans by lactobacillus plantarum isolates from fermenting cereals, Nigerian food journal enzymatic. Niger Food J 2013; 31(2): 84-90.
[http://dx.doi.org/10.1016/S0189-7241(15)30080-1]
[17]
Sokrab AM, Mohamed Ahmed IA, Babiker EE. Effect of fermentation on antinutrients, and total and extractable minerals of high and low phytate corn genotypes. J Food Sci Technol 2014; 51(10): 2608-15.
[http://dx.doi.org/10.1007/s13197-012-0787-8] [PMID: 25328202]
[18]
Amadi PU, Ifeanacho MO, Agomuo EN. The effects of different heating periods and exclusion of some fermentation conditions on bioethanol production from plantain pseudo-stem waste using the digestive juice of Archachatina marginata, garlic and Saccharomyces cerevisiae. Biofuels 2017; 9(4): 531-9.
[http://dx.doi.org/10.1080/17597269.2017.1292018]
[19]
Amadi PU, Ifeanacho MO. Impact of changes in fermentation time, volume of yeast, and mass of plantain pseudo-stem substrate on the simultaneous saccharification and fermentation potentials of African land snail digestive juice and yeast. J Genet Eng Biotechnol 2016; 14(2): 289-97.
[http://dx.doi.org/10.1016/j.jgeb.2016.09.002] [PMID: 30647627]
[20]
Jendrassik L, Groff P. Colorimetric method for measurement of bilirubin. Biochem J 1938; 297: 81.
[21]
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95(2): 351-8.
[http://dx.doi.org/10.1016/0003-2697(79)90738-3] [PMID: 36810]
[22]
Manchali S, Chidambara MKN, Patil BS. Crucial facts about health benefits of popular cruciferous vegetables. J Funct Foods 2012; 4: 94-106.
[http://dx.doi.org/10.1016/j.jff.2011.08.004]
[23]
Aebi H. Catalase in vitro. Methods Enzymol 1984; 105(C): 121-6.
[http://dx.doi.org/10.1016/S0076-6879(84)05016-3] [PMID: 6727660]
[24]
Krohne-Ehrich G, Schirmer RH, Untucht-Grau R. Glutathione reductase from human erythrocytes. Isolation of the enzyme and sequence analysis of the redox-active peptide. Eur J Biochem 1977; 80(1): 65-71.
[http://dx.doi.org/10.1111/j.1432-1033.1977.tb11856.x] [PMID: 923580]
[25]
Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974; 249(22): 7130-9.
[PMID: 4436300]
[26]
Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70(1): 158-69.
[PMID: 6066618]
[27]
Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 1968; 25(1): 192-205.
[http://dx.doi.org/10.1016/0003-2697(68)90092-4] [PMID: 4973948]
[28]
Greenlee L, Handler P. Xanthine oxidase. IV. Influence of pH on substrate specificity. J Biol Chem 1964; 239: 1090-5.
[PMID: 14165912]
[29]
Strittmatter CF. Studies on avian xanthine dehydrogenases: properties and patterns of appearance during development. J Biol Chem 1965; 240: 2557-64.
[PMID: 14304867]
[30]
Weatherburn MW. Phenol-hypochlorite reaction for determination of ammonia. Anal Chem 1967; 39: 971-4.
[http://dx.doi.org/10.1021/ac60252a045]
[31]
Mentzer WC, August CS, Nathan DG. The effects of androgen administration in sickle cell anemia. Pediatr Res 1969; 3: 378.
[32]
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-54.
[http://dx.doi.org/10.1016/0003-2697(76)90527-3] [PMID: 942051]
[33]
Ohls R, Maheshwari A. Hematology, immunology and infectious disease neonatology questions and controversies. The Netherlands: Elsevier 2012.
[34]
Newburger PE, Dale DC. Evaluation and management of patients with isolated neutropenia. Semin Hematol 2013; 50(3): 198-206.
[http://dx.doi.org/10.1053/j.seminhematol.2013.06.010] [PMID: 23953336]
[35]
Purves WK, Sadava D, Orians GH, Heller HC. Life: the science of biology. 7th ed. United States: W.H. Freeman 2004.
[36]
Agomuo E, Amadi P, Ogunka-Nnoka C, Amadi B, Ifeanacho M, Njoku U. Characterization of oils from Duranta repens leaf and seed. Oil Seeds Crops Lipids 2017; 24(6): 1-8.
[http://dx.doi.org/10.1051/ocl/2017048]
[37]
Weiss SL, Deutschman CS. Elevated malondialdehyde levels in sepsis - something to ‘stress’ about? Crit Care 2014; 18(2): 125.
[http://dx.doi.org/10.1186/cc13786] [PMID: 25029036]
[38]
Ogunka-Nnoka CU, Amagbe R, Amadi BA, Amadi PU. Biochemical effects of Telfairia occidentalis leaf extracts against copper-induced oxidative stress and histopathological abnormalities. J Adv Med Pharma Sci 2017; 12(2): 1-15.
[http://dx.doi.org/10.9734/JAMPS/2017/31295]
[39]
Ishizaka Y, Yamakado M, Toda A, Tani M, Ishizaka N. Relationship between estimated glomerular filtration rate, albuminuria, and oxidant status in the Japanese population. BMC Nephrol 2013; 14: 191.
[http://dx.doi.org/10.1186/1471-2369-14-191] [PMID: 24016221]
[40]
Wayne LM, Daniel DB, Aaron G. Dietary sodium modulation of aldosterone activation and renal function during the progression of experimental heart failure miller: dietary sodium and early heart Failure. Eur J Heart Fail 2015; 17(2): 144-50.
[http://dx.doi.org/10.1002/ejhf.212]
[41]
Sayama K, Imagawa A, Okita K, Uno S, Moriwaki M, Kozawa J, et al. Pancreatic beta and alpha cells are both decreased in patients with fulminant type 1 diabetes: a morphometrical assessment. Diabetologia 2005; 48(8): 1560-4.
[http://dx.doi.org/10.1007/s00125-005-1829-9] [PMID: 15991022]
[42]
McCrimmon R. The mechanisms that underlie glucose sensing during hypoglycaemia in diabetes. Diabet Med 2008; 25(5): 513-22.
[http://dx.doi.org/10.1111/j.1464-5491.2008.02376.x] [PMID: 18312421]
[43]
Gerich JE, Langlois M, Noacco C, Karam JH, Forsham PH. Lack of glucagon response to hypoglycemia in diabetes: evidence for an intrinsic pancreatic alpha cell defect. Science 1973; 182(4108): 171-3.
[http://dx.doi.org/10.1126/science.182.4108.171] [PMID: 4581053]
[44]
Naleid AM, Grace MK, Cummings DE, Levine AS. Ghrelin induces feeding in the mesolimbic reward pathway between the ventral tegmental area and the nucleus accumbens. Peptides 2005; 26(11): 2274-9.
[http://dx.doi.org/10.1016/j.peptides.2005.04.025] [PMID: 16137788]
[45]
Soriano-Guillén L, Barrios V, Lechuga-Sancho A, Chowen JA, Argente J. Response of circulating ghrelin levels to insulin therapy in children with newly diagnosed type 1 diabetes mellitus. Pediatr Res 2004; 55(5): 830-5.
[http://dx.doi.org/10.1203/01.PDR.0000120679.92416.70] [PMID: 14973181]
[46]
Azar ST, Zalloua PA, Zantout MS, Shahine CH, Salti I. Leptin levels in patients with type 1 diabetes receiving intensive insulin therapy compared with those in patients receiving conventional insulin therapy. J Endocrinol Invest 2002; 25(8): 724-6.
[http://dx.doi.org/10.1007/BF03345107] [PMID: 12240905]
[47]
Hotamisligil GS, Arner P, Caro JF, Atkinson RL, Spiegelman BM. Increased adipose tissue expression of tumor necrosis factor-alpha in human obesity and insulin resistance. J Clin Invest 1995; 95(5): 2409-15.
[http://dx.doi.org/10.1172/JCI117936] [PMID: 7738205]
[48]
Rath PC, Aggarwal BB. TNF-induced signaling in apoptosis. J Clin Immunol 1999; 19(6): 350-64.
[http://dx.doi.org/10.1023/A:1020546615229] [PMID: 10634209]
[49]
Miyazaki Y, Pipek R, Mandarino LJ, DeFronzo RA. Tumor necrosis factor alpha and insulin resistance in obese type 2 diabetic patients. Int J Obes Relat Metab Disord 2003; 27(1): 88-94.
[50]
Elmarakby AA, Sullivan JC. Relationship between oxidative stress and inflammatory cytokines in diabetic nephropathy. Cardiovasc Ther 2012; 30(1): 49-59.
[http://dx.doi.org/10.1111/j.1755-5922.2010.00218.x] [PMID: 20718759]
[51]
Maeda N, Shimomura I, Kishida K, Nishizawa H, Matsuda M, Nagaretani H, et al. Diet-induced insulin resistance in mice lacking adiponectin/ACRP30. Nat Med 2002; 8(7): 731-7.
[http://dx.doi.org/10.1038/nm724] [PMID: 12068289]
[52]
Havel PJ. Update on adipocyte hormones: regulation of energy balance and carbohydrate/lipid metabolism. Diabetes 2004; 53(Suppl. 1): S143-51.
[http://dx.doi.org/10.2337/diabetes.53.2007.S143] [PMID: 14749280]
[53]
Liadis N, Murakami K, Eweida M, Elford AR, Sheu L, Gaisano HY, et al. Caspase-3-dependent beta-cell apoptosis in the initiation of autoimmune diabetes mellitus. Mol Cell Biol 2005; 25(9): 3620-9.
[http://dx.doi.org/10.1128/MCB.25.9.3620-3629.2005] [PMID: 15831467]
[54]
Agosto M, Azrin M, Singh K, Jaffe AS, Liang BT. Serum caspase-3 p17 fragment is elevated in patients with ST-segment elevation myocardial infarction: a novel observation. J Am Coll Cardiol 2011; 57(2): 220-1.
[http://dx.doi.org/10.1016/j.jacc.2010.08.628] [PMID: 21211695]
[55]
Djemli-Shipkolye A, Coste T, Raccah D, Vague P, Pieroni G, Gerbi A. Na, Katpase alterations in diabetic rats: relationship with lipid metabolism and nerve physiological parameters. Cell Mol Biol 2001; 47(2): 297-304.
[PMID: 11355004]
[56]
Chen D, Song M, Mohamad O, Yu SP. Inhibition of Na+/K+-ATPase induces hybrid cell death and enhanced sensitivity to chemotherapy in human glioblastoma cells. BMC Cancer 2014; 14: 716.
[http://dx.doi.org/10.1186/1471-2407-14-716] [PMID: 25255962]
[57]
Stewart J, Turner KJ. Inhibin B as a potential biomarker of testicular toxicity. Cancer Biomark 2005; 1(1): 75-91.
[http://dx.doi.org/10.3233/CBM-2005-1109] [PMID: 17192034]
[58]
Xiong X, Zhong A, Xu H. Effect of cyanotoxins on the hypothalamic-pituitary-gonadal axis in male adult mouse. PLoS One 2014; 9(11) e106585
[http://dx.doi.org/10.1371/journal.pone.0106585] [PMID: 25375936]
[59]
Peltola V, Huhtaniemi I, Metsa-Ketela T, Ahotupa M. Induction of lipid peroxidation during steroidogenesis in the rat testis. Endocrinology 1996; 137(1): 105-12.
[http://dx.doi.org/10.1210/endo.137.1.8536600] [PMID: 8536600]
[60]
Oner-Iyidoğan Y, Gürdöl F, Oner P. The effects of acute melatonin and ethanol treatment on antioxidant enzyme activities in rat testes. Pharmacol Res 2001; 44(2): 89-93.
[http://dx.doi.org/10.1006/phrs.2001.0828] [PMID: 11516256]
[61]
Hannon PR, Flaws JA. The effects of phthalates on the ovary. Front Endocrinol 2015; 6: 8.
[http://dx.doi.org/10.3389/fendo.2015.00008] [PMID: 25699018]
[62]
Bagur AC, Mautalen CA. Risk for developing osteoporosis in untreated premature menopause. Calcif Tissue Int 1992; 51(1): 4-7.
[http://dx.doi.org/10.1007/BF00296207] [PMID: 1393775]
[63]
Sowers MR, La Pietra MT. Menopause: its epidemiology and potential association with chronic diseases. Epidemiol Rev 1995; 17(2): 287-302.
[http://dx.doi.org/10.1093/oxfordjournals.epirev.a036194] [PMID: 8654512]
[64]
Okereke C, Onuoha S. Effect of ethanolic extract of Cannabis sativa on progesterone and estrogen hormones in female Wistar Rats. Reprod Syst Sex Disord 2015; 4: 2.
[http://dx.doi.org/10.4172/2161-038X.1000150]
[65]
Mancini T, Casanueva FF, Giustina A. Hyperprolactinemia and prolactinomas. Endocrinol Metab Clin North Am 2008; 37(1): 67-99.
[http://dx.doi.org/10.1016/j.ecl.2007.10.013]
[66]
Jørgensen H, Knigge U, Kjaer A, Vadsholt T, Warberg J. Serotonergic involvement in stress-induced ACTH release. Brain Res 1998; 811(1-2): 10-20.
[http://dx.doi.org/10.1016/S0006-8993(98)00901-9] [PMID: 9804868]


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VOLUME: 14
ISSUE: 1
Year: 2020
Page: [16 - 32]
Pages: 17
DOI: 10.2174/1872208313666190822144729
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