Objective: In this study, chitosan/alginate-ellagic acid sustained-release microspheres were
prepared, and the effect of sustained-release microspheres on preadipocyte adipogenic differentiation
Methods: Chitosan/alginate-ellagic acid microspheres were prepared and identified by scanning electron
microscopy (SEM) and infrared spectroscopy (IR). The drug release rates were measured at pH
6.8, 7.0, 7.2, 7.4 to determine sustained release of ellagic acid from microspheres. The effects of 0.1, 1,
10 mg/L chitosan/alginate-ellagic acid microsphere on 3T3-F442A preadipocyte proliferation were determined
by Methyl thiazolyl tetrazolium assay (MTT), and cell morphology was checked by hematoxylin/
eosin staining (HE staining). The effect of chitosan/alginate-ellagic acid microspheres on
preadipocyte adipogenic differentiation was also determined by Oil red O staining, and lipogenesis was
measured by isopropanol extraction. The molecular mechanism was investigated by detecting the
mRNA expression of CCAAT/enhancer binding protein alpha (C/EBPα) and peroxisome proliferatorsactivated
receptor gamma (PPARγ).
Results: Chitosan/alginate-ellagic acid sustained-release microspheres were successfully prepared, and
the inhibition of proliferation and adipogenic differentiation of preadipocytes was found to be dosedependent.
The mechanism of differentiation inhibition was found to be closely related to the expression
of transcription factor C/EBPα and PPARγ.
Conclusion: Chitosan/alginate can be used as a good material to prepare ellagic acid sustained-release
microspheres, and these microspheres can be used for treating the obesity.