Background: The medicinal plants have enormous pharmacological properties with
fewer side effects. Today, there is an increasing demand of medicinal plants as an anti-aging and
Objective: The aim of this study is to evaluate the antioxidant, anti-aging and anti-wrinkle potential
of Salvia officinalis.
Materials and Methods: Salvia officinalis (Lamiaceae) is folk medicine of Asia and Latin America.
Powdered crude drug 100 g was successively extracted in a soxhlet apparatus with petroleum
ether (60-80ºC), chloroform and methanol. After successive solvents, extraction methanolic extract
was used for testing of antioxidant potential using DPPH assay. Further, the antiaging potential of
the extract was investigated by the inhibitory effect of various enzymatic estimations i.e. Col-I, Ela-
I and Hya-I inhibitory assays on early aging human skin fibroblasts. The antiwrinkle potential of
plant Salvia officinalis was done by using a UV light-induced photoaging model.
Results: Phytochemical analysis showed the presence of glycosides, alkaloids flavonoids, and
triterpenoids, saponins and Phenolic Compounds at high level. The extract showed inhibitory concentration
(IC50: 24.65) and ascorbic acid. The standard antioxidant showed inhibitory concentration
(IC50: 20.10). In enzymatic estimations assay, the Col-I, Ela-I and Hya-I of extract were assessed
showing inhibitory concentration as Col-I (IC50:21.36), Ela-I (IC50:35.05) and Hya-I
(IC50:23.44), respectively. Thus, MeOH extract of Salvia officinalis can inhibit 50% of the activity
of aging-related enzymes Col-I, Ela-I and Hya-I. The wrinkle score of negative control i.e. UV treated
group was 2.83 ± 0.408, and MeOH extract of Salvia officinalis treated group is 1.83 ± 0.753.
Conclusion: This study concluded that MeOH extract of Salvia officinalis has confirmed the high
antioxidant potential and in vitro and in vivo inhibitory potential of antiaging enzymes assessed,
thus they could be used for further development of cosmetic products and nutraceuticals.