Study of the Fluorescence based Applications of Water Soluble (N, P) Doped Carbon Dots Synthesized via Microwave Assisted Green Pyrolysis

Author(s): Rajshree Singh, Shikha Singh, Sudipta Senapati, Kheyanath Mitra, Jaydeep Singh, Susanta K.S. Gupta, Nira Misra, Pralay Maiti, Biswajit Ray*

Journal Name: Nanoscience & Nanotechnology-Asia

Volume 10 , Issue 6 , 2020

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Graphical Abstract:


Background: Water soluble nitrogen and phosphorus doped carbon dots (CD) have been synthesized using citric acid, tris(2-aminoethyl)amine and orthophosphoric acid via one step microwave assisted pyrolytic method.

Methods: The CD synthesized has been characterized using FTIR, UV-Vis, fluorescence spectroscopy and EDAX coupled with SEM techniques.

Results: SEM study has shown the formation of nanosized CD with an average size of ~18 nm. Elemental analysis via EDAX has confirmed successful incorporation of nitrogen (30.8% wt) and phosphorus (5.7% wt) atoms in it. The steady state and 3D fluorescence spectroscopic studies have shown its efficient fluorescence emission with emission maxima in the region of ~450 nm. It has shown efficient “turn off” fluorescence behaviour towards transition metal ions like Fe2+ and Co2+ ions and toxic nitrophenolic compounds like p-nitrophenol and picric acid. Its efficient interaction with BSA has been revealed in terms of fluorescence quenching of BSA by steady state, synchronous and 3D fluorescence spectroscopy. It has shown very good in vitro biocompatibility and enhanced cell adhesion properties towards NIH 3T3 fibroblast cells. Moreover, fluorescence microscopy has shown significant uptake of CD by the tested cell line.

Conclusion: Such bio-compatible nitrogen and phosphorous doped CD can be potentially useful to estimate metal ions, p-nitrophenol and picric acid using fluorescence spectroscopy and for fluorescence based bio-imaging.

Keywords: Carbon dots, green pyrolysis, cell adhesion, fibroblast cells, nano-materials, bioimaging.

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Article Details

Year: 2020
Published on: 30 November, 2020
Page: [827 - 839]
Pages: 13
DOI: 10.2174/2210681209666190807151604
Price: $25

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