Background: A major focus of nanotechnology concerns is the expansion of the optimization
of nanomaterials in purity, size and dispersity.
Methods: In the current work, a two-step AgNP synthesis process was optimized at the mycelia-DI
water suspension and AgNP formation reaction levels.
Results: Biomass filtrate from the fungal strain Tritirachium oryzae W5H was able to reduce silver
nitrate into AgNPs after a 72 h reaction, as indicated by the development of intense brown color and
by UV-vis spectra. The biosynthesis ability of AgNPs was markedly better in the presence of a single
carbon and nitrogen source in the culture medium compared to multiple sources of carbon and nitrogen.
The optimization results of AgNP formation were indifferent between the two steps and were 20
g biomass, 40°C, pH 7.0, 96 h and 1.0 mM AgNO3. The TEM images of the prepared AgNPs illustrated
the presence of 7-75 nm, monodispersed and spherical- to ovular-shaped Ag nanoparticles.
Conclusion: The present work highlights the importance of investigating the process parameters by
which the reductant mycelia-free filtrate was prepared. In addition, we explored the promising antibacterial
action of the prepared AgNPs against bacterial infections.