Background: Functional and structural diversity of proteins of snake venoms is coupled with a
wide repertoire of pharmacological effects. Snake venoms are targets of studies linked to searching molecules
with biotechnological potential.
Methods: A homologue phospholipase A2 (BmatTX-IV) was obtained using two chromatographic techniques.
Mass spectrometry and two-dimensional gel electrophoresis were used to determine the molecular
mass and isoelectric point, respectively. By means of Edman degradation chemistry, it was possible to
obtain the partial sequence of amino acids that comprise the isolated toxin. Trypanocidal, leishmanicidal
and cytoxic activity against Trypanosoma cruzi, Leishmania infantum and murine fibrobasts was determinated.
Results: Combination of both chromatographic steps used in this study demonstrated efficacy to obtain
the PLA2-Lys49. BmatTX-IV showed molecular mass and isoelectric point of 13.55 kDa and 9.3, respectively.
Amino acid sequence of N-terminal region (51 residues) shows the presence of Lys49 residue at
position 49, a distinctive trait of enzymatically inactive PLA2. Bothrops mattogrossensis snake venom
showed IC50 values of 11.9 μg/mL against Leishmania infantum promastigotes and of 13.8 μg/mL against
Trypanosoma cruzi epimastigotes, respectively. On the other hand, the venom showed a high cytotoxic
activity (IC50 value of 16.7 μg/mL) against murine fibroblasts, whereas the BmatTX-IV showed IC50
value of 81.2 μg/mL.
Conclusion: Physicochemical and biological characterization of snake venoms components is critically
important, since these complex mixtures provide a source of molecules with antiparasitic potential, making
further studies necessary to identify and characterize components with higher efficacy and selectivity.