Objective: Andrographolide has potent anticancer and antimicrobial activity; however, its
clinical application has been limited due to its poor water solubility as well as lack of appropriate formulation.
The objective of this investigation was to formulate Self–Micro Emulsifying Drug Delivery
System (SMEDDS) of andrographolide and explore its oral drug delivery aptitudes.
Methods: Andrographolide SMEDDS was optimized by ternary phase approach and studied for various
in vitro characteristics: Particle size, electron microscopy, polydispersity index, surface charge, dilution
effect, pH stability, freeze-thaw effect, dissolution profile and stability studies. Further, antimicrobial
and cytotoxic performance of andrographolide SMEDDS were evaluated in MCF–7 breast cancer cell
lines and methicillin-resistant microorganisms, respectively.
Results: An optimized SMEDDS formulation of andrographolide was successfully prepared and evaluated
for its drug delivery potential. The solubility of andrographolide in the developed SMEDDS formulation
was increased significantly, and the drug loading was enough for making this drug clinically
applicable. The andrographolide SMEDDS formulation competitively inhibited the growth of microorganisms
and showed enhanced anti–microbial activity against MRSA microorganisms.
Conclusions: The SMEDDS strategy represents one of the best approaches to deliver andrographolide
via oral route, while resolving its solubility limitations.