Background: Src associated with mitosis of 68 kDa (Sam68), is often highly expressed in
human cancers. Overexpression of Sam68 has been shown to be correlated with poor survival prognosis
in some cancer patients. However, little is known whether Sam68 plays a role in promoting
metastasis in breast cancer.
Materials and Methods: The expression of Sam68 protein in breast cancer tissue was detected by
immunohistochemistry. Trans-well assay, wound-healing, real-time PCR and Western blotting
analysis were used to detect the effect of Sam68 on promoting EMT or metastasis of breast cancer.
Next-generation RNA sequencing was used to analyze genes that may be regulated by Sam68.
Results: Sam68 plays a positive role in promoting breast cancer metastasis. Sam68 was found to be
overexpressed in breast cancer along with lymph node metastasis. MMP-9 was also found to be
overexpressed in breast cancer tissue and was correlated to the expression of Sam68 (P<0.01).
Xenograft in NOD/SCID mice and in vitro experiments confirmed that the invasion and metastatic
ability of breast cancer cells were regulated by Sam68. And EPHA3 could be up-regulated by
Sam68 in breast cancer.
Conclusion: High expression of Sam68 participates in breast cancer metastasis by up-regulating the