Background: Marine sponges provided a great source of natural products with promising
biological activity. This study was aimed to investigate the chemical constituents of methanol extracts
of selected Indonesian marine sponges (Callyspongia sp., Clathria sp., Melophlus sarasinorum,
and Xestospongia sp.), collected from the Saponda Islands, Southeast Sulawesi, Indonesia as
well as to evaluate their antimicrobial and antioxidant activities.
Methods: LCMS/MS analysis used to identify the compounds. Agar well diffusion and DPPH
assays were used to evaluate the antimicrobial and antioxidant activities.
Results: Chemical screening reported alkaloids, terpenoids, steroids, and saponins from all investigated
sponges. The LC-MS/MS analysis identified various compounds which mainly contained steroids.
Antimicrobial activity (against Bacillus subtilis, Escherichia coli, Salmonella enterica, and
Candida albicans) was only shown by the Xestospongia sp. extract. Meanwhile, extracts of M.
sarasinorum, Xestospongia sp., and Callyspongia sp. exhibited potent radical scavenging activity.
Conclusion: The study concluded that the selected sponges could provide various groups of
compounds. Methanol extracts of these sponges could be used as sources of antimicrobial and