Title:Investigation of Antioxidant Properties of Three Naturally Isolated Microalgae: Identification and Bioinformatics Evaluation of the Most Efficient Strain
VOLUME: 13 ISSUE: 4
Author(s):Mohammad Hossein Morowvat*, Sara Goharian and Younes Ghasemi
Affiliation:Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, P.O. Box 71345-1583, Shiraz, Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, P.O. Box 71345-1583, Shiraz, Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, P.O. Box 71345-1583, Shiraz
Keywords:Antioxidant, bioinformatic analysis, microalgae, naturally isolated, polyphenol content, folinciocalteu
analysis.
Abstract:
Background and Objectives: In this study, three naturally isolated species of microalgae
were investigated for their antioxidant activity using DPPH assay and polyphenol contents
through Folin-ciocalteu analysis.
Methods: Three different solvents including hexane, ethyl acetate and water, which present different
polarities were utilized to extract the bioactive components from three different microalgal
strains.
Results: The investigated species exhibited considerable amounts of antioxidants (25.88 ± 0.48
µmol Trolox g-1 in Scenedesmus obliquus) and polyphenol compounds (27.14 ± 0.40 mg GAE g-1
in Chlorella vulgaris). The observed correlation coefficient (R2=0.8265) between the two studied
parameters confirmed the great contribution of the phenolic compounds in the observed antioxidant
activities.
Conclusion: The phylogenetics and other bioinformatic tools were confirmed as a useful method
for identification of the most prominent species. The obtained data and patents warrant the potentials
of naturally isolated microalgal strains for obtaining different antioxidant and polyphenolic
bioactive compounds.