Background: Herein, we report the biosynthesis procedure to prepare silver nanoparticles as
reduction and capping agents with the aqueous plant extract of Perovskia abrotanoides.
Methods: The therapeutic application of silver nanoparticles entirely depends on the size and shape of
the nanoparticles therefore, their control during the synthesis procedure is so important. The effects of
synthesis factors, for example, silver ion concentration, the mass of plant extract, reaction time and extraction
temperature, on the size of silver particles were considered and optimized. Several analytical
methods were used for the characterization of silver NPs including FT-IR and UV–Vis spectrophotometer,
XRD and SEM.
Results: The results showed that the mean size of the silver particles was about 51 nm. Moreover, the
antibacterial properties of biosynthesized silver NPs were investigated by the minimum inhibitory concentration,
minimum bactericidal concentration, and Well-diffusion tests. The minimum inhibitory concentration/
minimum bactericidal concentration values of silver NPs and aqueous plant extract versus
Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) and Gram-negative bacteria (E. coli)
were 3.03/0.00, 1.20/0.01, 3.06/0.00, 0.98/1.04, 1.00/0.05 and 1.30/0.03 (mg/mL), respectively.
Conclusion: The antimicrobial activity study displayed that the synthesized silver nanoparticles by
plant extract have better antimicrobial properties compared to aqueous plant extract of Perovskia abrotanoides.