Heterocyclic compounds constitute one of the largest and most versatile family of organic compounds. Today there are many heterocyclic compounds that are being isolated from natural sources and day by day the number is increasing rapidly due to their enormous utility. Nitrogen containing heterocyclic compounds have a prominent role in most fields of sciences such as medicinal chemistry, biochemistry also another area of sciences. In this review, we cover most biological active nitrogen containing heterocyclic compounds that are extracted from the natural sources including indole, Carbazole, Quinoline, Isoquinoline and Benzthiazole ring system. These isolated nitrogen containing heterocyclic compounds renders antifungal, anti-inflammatory, antibacterial, antioxidants, anticonvulsant, antiallergic, herbicidal and anticancer properties.