Background: Phage display is a biotechnological technique that presents peptides with
coated proteins on the surface of phage. In the last two decades, growing applications of phage display
in various fields of biotechnology have been investigated. Phage display libraries allow to present
billions of peptides on phage surface for selection of a specific peptide with the desired affinity.
Objective: In this regard, high-affinity phage antibodies against tumor antigens are produced and
applied for diagnosis and treatment of cancer.
Methods: Moreover, phage display libraries are employed to select the high affinity T Cell Receptors
(TCRs) for the peptide-MHC complex which is an attractive approach in cancer immunotherapy. Due to
immunogenic properties of phage particles, phage-based vaccines do not require adjuvant, in addition the
phage particles can effectively take up by Antigen Presenting Cells (APCs).
Results: Taken together, phage-based cancer vaccines are ideal candidates that provide a key for
eradication of tumor cells.
Conclusion: In this review, we focus on various applications of a phage display platform in different
types of cancer immunotherapy approaches.