Endocrine disrupting compounds (EDCs) have the potential to cause adverse effects on
wildlife and human health. Two important EDCs are the synthetic estrogen 17α-ethynylestradiol
(EE2) and bisphenol-A (BPA) both of which are xenoestrogens (XEs) as they bind the estrogen receptor
and disrupt estrogen physiology in mammals and other vertebrates. In the recent years the influence
of XEs on oncogenes, specifically in relation to breast and prostate cancer has been the subject of
Methodology: In this study, healthy primary human prostate epithelial cells (PrECs) were exposed to
environmentally relevant concentrations of BPA (5nM and 25nM BPA) and interrogated using a
whole genome microarray.
Results: Exposure to 5 and 25nM BPA resulted in 7,182 and 7,650 differentially expressed (DE)
genes, respectively in treated PrECs. Exposure to EE2 had the greatest effect on the PrEC transcriptome
(8,891 DE genes).
Conclusion: We dissected and investigated the nature of the non-estrogenic gene signature associated
with BPA with a focus on transcripts relevant to epigenetic modifications. The expression of transcripts
encoding nuclear hormone receptors as well as histone and DNA methylation, modifying enzymes
were significantly perturbed by exposure to BPA.