Background: Commelina communis Linn (Commelinae Herba) is a traditional Chinese medicine
that can be used both as food and as medicine. It has been used to treat a variety of disorders, including
a cold, high fever, sore throat, edema and oliguria for many years. Two pairs of isomeric flavonoid
glycosides are the main active components in Commelina communis Linn, and they have a high
Objective: The objective of this study was to determine the pharmacodynamic and pharmacological
effects of Commelina communis Linn.
Method: A sensitive, efficient, and rapid LC-MS/MS method was developed to simultaneously identify
two pairs of isomeric flavonoid glycosides in rats. Chromatographic separation was carried out on a
Wonda Cract ODS-2 C18 column (150 mm x 4.6 mm, 5 μm) using a mobile phase composed of 0.1%
formic acid (aqueous solution) and methanol at a flow rate of 0.8 mL/min. The detection of the four
analytes and the internal standard (IS) sulfamethoxazole was performed with multiple reaction monitoring
(MRM) in negative electrospray ionization mode. All the analytes were eluted within 20 min.
Results: This method was successfully applied for simultaneous identification of the concentrations of
the four compounds in the plasma after the oral administration of 10 mL/kg Commelina communis Linn
extract to rats. The pharmacokinetic study indicated that analytes reached their Cmax in approximately 15
min and could be detected until 12 h.
Conclusion: The method complies with the State Food and Drug Administration guidelines for selectivity,
sensitivity, accuracy, precision, matrix effect, extraction recovery and stability. This is the first report
on the pharmacokinetics of Commelina communis Linn. The information gained from this research
may be valuable for the preclinical and clinical applications of Commelina communis Linn.