Characterization of 10 Biogenic Amines in Male Silkworm Moth by HPLC with Precolumn Derivatization

Author(s): Mingqin Fan, Yun Ai, Wenjie Zhao, Yanni Sun, Jianli Liu, Ning Zhang*

Journal Name: Current Pharmaceutical Analysis

Volume 16 , Issue 5 , 2020


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Graphical Abstract:


Abstract:

Background: Biogenic Amines (BAs) are biologically active nitrogenous organic compounds of low molecular weight, which are frequently found in a wide variety of foods, beverages and herbs due to their toxic potential in humans. Male Silkworm Moth (MSM), a Traditional Chinese Medicine (TCM), has been exploited and utilized as nutritious liquor based on its traditional effects in the Chinese community.

Objective: The objective of this study was to develop an HPLC with Dns-Cl derivatization method for characterizing overall BAs in MSM and providing data for further evaluating its activities and safety profiles.

Methods: The method has acceptable sensitivity, precision, accuracy, selectivity and recovery, and was successfully applied to the determination of the BAs contents in MSM for the first time.

Results: In the analysis of 10 batches of MSM samples, serotonin and dopamine were not found in detectable concentrations in any samples, and the most abundant amine found was putrescine. The mean values of tryptamine, phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine, and spermine determined in the samples were found to be 34.7 mg/ kg, 16.1 mg/ kg, 218.3 mg/ kg, 37.9 mg/ kg, 12.1 mg/ kg, 18.2 mg/ kg, 4.5mg/ kg, and 0.9 mg/ kg, respectively.

Conclusion: The contents of BAs in 10 batches of MSM were below the maximum recommended limits, and MSM can be used safely.

Keywords: Biogenic amines, Male silkworm moth, Derivatization, HPLC, Dns-Cl, safety profiles.

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Article Details

VOLUME: 16
ISSUE: 5
Year: 2020
Published on: 15 June, 2020
Page: [608 - 614]
Pages: 7
DOI: 10.2174/1573412915666190522090831
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