Objective: In the present study, an attempt was made to synthesize biodegradable, hemocompatible,
antimicrobial and pH-responsive hydrogel.
Methods: Microwave facilitated green synthesis was carried out for the grafting of acrylamide over
Kheri Gum Polysaccharide (KGP) backbone. The grafted hydrogel was characterized in terms of
FTIR spectra, 1H NMR spectra, SEM image, contact angle, chemical resistant, biodegradation, blood
clotting time, thrombogenicity, hemolytic activity and cytotoxic effect.
Results: More swelling was observed by graft copolymers (KGP-g-Am) in all the solvent systems
such as double distilled water, 1 N NaOH and 0.1 N HCl than KGP. Graft copolymers showed more
swelling in 1 N NaOH than in 0.1 N HCl, followed by distilled water. Blood clotting studies showed
longer clotting time for KGP-g-Am as compared to the uncoated glass surface and KGP coated glass
surface. Results obtained after molecular docking predict that TLR-4 receptors are considerably
more liable than TLR-2 receptors for antimicrobial activity of both KGP-g-Am and KGP. Experimental
data evidently explains the better antimicrobial efficacy of KGP-g-Am (K1) against Escherichia
coli and Aspergillus niger than KGP. In molecular docking studies, KGP-g-Am showed prominent
anticancer activity than KGP at the protease-activated receptor (PAR1). Results of in vitro cytotoxic
activity against breast cancer cell lines (MCF 7) predict better control over cell growth by
KGP-g-Am (K1) as compared to KGP.
Conclusion: It can be elicited from the data that microwave assisted grafting over KGP backbone
modulates and introduces prerequisite properties within the polymer and can be utilized for various
biomedical, pharmaceutical and cosmeceutical applications.