Background: Among the existing antifungal drugs, Amphotericin B is the first drug in
the treatment of systemic fungal infections. However, its large adverse reactions limit the clinical
application and Liposome Amphotericin B resolves the problem.
Objective: In the present study, a rapid, simple, sensitive and efficient method based on LCMS/
MS for determination of liposomal Amphotericin B in rat plasma and tissue samples using
natamycin as the internal standard has been developed and validated.
Methods: The analytical samples contain the plasma and various tissues disposed of by protein
precipitation and determination of liposomal Amphotericin B by an LC-MS/MS. Chromatographic
separation was achieved on a Poroshell 120 EC-C18 column (4.6 mm × 50 mm, 2.7 μm) with 10
mmol/L ammonium acetate in water-acetonitrile by gradient elution at a flow rate of 0.7 mL/min.
The MS analysis was conducted in positive electrospray ionization with Multiple Reaction Monitoring
Results: The calibration curves of plasma and tissues showed good linear range from 50 to 10000
ng/mL. The analytical samples containing plasma and tissues were stable under different storage
conditions and temperature.
Conclusion: The developed LC-MS/MS method has been successfully applied to the studies of
toxicokinetics and tissue distribution after intravenous injection of liposomal Amphotericin B to