Background: Deltamethrin (DLM) is a type 2 pyrethroid insecticide used in agriculture and
home to control pests. However, emerging reports have indicated the immunotoxicity of DLM.
Objective: Thus, in the current investigation, we have checked the immune-protective role of quercetin
in DLM-induced immunotoxicity by using in silico and in vitro techniques.
Results: In silico results have shown good interaction of quercetin towards immune cell receptors (T &
B cell receptors). The findings of in vitro studies indicated the decrease in oxidative stress which is
elevated by DLM in concentration & time-dependent manner. The increased caspases-3 activity was
decreased by treatment of quercetin. The apoptosis induced by DLM in thymus and spleen was suppressed
only at higher concentration (50μg/ml) of quercetin. Finally, the phenotypic changes due to
DLM were restored by quercetin.
Conclusion: Quercetin has strong binding affinity towards CD4, CD8 and CD28, CD45 receptors and
protects the thymocytes and splenocytes against DLM-induced apoptotic signaling pathways.