PCR: An Effective, Faster and Relevant Method for the Detection of Clinical Isolates of Candida albicans

Author(s): Kamal Uddin Zaidi, Abin Mani*, Richa Parmar, Vijay Thawani

Journal Name: Current Drug Therapy

Volume 15 , Issue 3 , 2020


DOI : 10.2174/1574885514666190409104833

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Graphical Abstract:


Abstract:

Background: Candida albicans is associated with infectious diseases with colonization, in both immunocompromised and immunocompetent patients. Antibiotic treatment can develop candidiasis.

Objective: Evaluation of ITS 4 and ITS5 primer expression on clinical isolates of C. albicans.

Methods: Fifty clinical isolates of C. albicans were collected from different sites viz. Catheter Tip (CT), High Vaginal Swab (HVS) and Urine (U).

Results: Using the universal fungal primers ITS4 and ITS5, PCR produced a single monomorphic amplification at 520 bp indicating that the strains are of same genetic identity.

Conclusion: This is an effective, faster and relevant method for the detection and characterization of clinically isolated strains of C. albicans.

Keywords: Primers, urine, extraction, candidiasis identification, antibiotic, monomorphic.

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Article Details

VOLUME: 15
ISSUE: 3
Year: 2020
Published on: 13 October, 2020
Page: [216 - 218]
Pages: 3
DOI: 10.2174/1574885514666190409104833
Price: $65

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