Title:PCR: An Effective, Faster and Relevant Method for the Detection of Clinical Isolates of <i>Candida albicans</i>
VOLUME: 15 ISSUE: 3
Author(s):Kamal Uddin Zaidi, Abin Mani*, Richa Parmar and Vijay Thawani
Affiliation:Biotechnology Pharmacology Laboratory and Human Genetics Laboratory, Centre for Scientific Research and Development, People’s University, Bhopal 462037, Biotechnology Pharmacology Laboratory and Human genetics Laboratory, Centre for Scientific Research and Development, People’s University, Bhopal 462037, Biotechnology Pharmacology Laboratory and Human genetics Laboratory, Centre for Scientific Research and Development, People’s University, Bhopal 462037, Biotechnology Pharmacology Laboratory and Human genetics Laboratory, Centre for Scientific Research and Development, People’s University, Bhopal 462037
Keywords:Primers, urine, extraction, candidiasis identification, antibiotic, monomorphic.
Abstract:Background: Candida albicans is associated with infectious diseases with colonization,
in both immunocompromised and immunocompetent patients. Antibiotic treatment can develop
candidiasis.
Objective: Evaluation of ITS 4 and ITS5 primer expression on clinical isolates of C. albicans.
Methods: Fifty clinical isolates of C. albicans were collected from different sites viz. Catheter Tip
(CT), High Vaginal Swab (HVS) and Urine (U).
Results: Using the universal fungal primers ITS4 and ITS5, PCR produced a single monomorphic
amplification at 520 bp indicating that the strains are of same genetic identity.
Conclusion: This is an effective, faster and relevant method for the detection and characterization
of clinically isolated strains of C. albicans.
