Introduction: Resveratrol and its monooxygenated metabolite oxyresveratrol
were the subject matter of intense research due to their medicinal value. Absorption, distribution,
metabolism and excretion are important to understand the bioavailability and pharmacokinetic
profile of resveratrol and oxyresveratrol. Quantification of resveratrol and
oxyresveratrol is essential for both in vitro and in vivo studies.
Methods: During in vitro drug metabolism studies, both qualitative and quantitative information
are essential to understand the metabolic profile of resveratrol and oxyresveratrol. In
the present study, a simple and stable method is outlined using high performance liquid
chromatography to quantify both resveratrol and oxyresveratrol. This method is suitable to
understand the metabolic stability, plasma stability, pharmacokinetics and toxicokinetics of
resveratrol and oxyresveratrol.
Results: Generally, in vitro incubation studies are performed at high concentrations and in
vivo studies are carried out at both high and low concentrations, therefore high performance
liquid chromatography method is demonstrated as a suitable technique to quantify resveratrol
Conclusion: Retention time of resveratrol and oxyresveratrol from liquid chromatography
qualitatively confirm its identity.