Pharmacokinetics of Benzoic Acid, 4-[[(2-Hydroxyethyl)Amino]Carbonyl]- Methyl Ester from Portulaca Oleracea L. in Rats after Intravenous and Oral Administrations Using UHPLC-ESI-Q-TOF/MS

Author(s): Haoran Xu, Zheming Ying, Lina Wang, Wenjie Zhang, Xixiang Ying*, Guanlin Yang*

Journal Name: Current Pharmaceutical Analysis

Volume 16 , Issue 5 , 2020


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Graphical Abstract:


Abstract:

Objective: The aim of this study is to investigate the pharmacokinetics of benzoic acid, 4- [[(2-hydroxyethyl)amino]carbonyl], methyl ester in rats after intravenous and oral administrations at doses of 3 mL/kg.

Methods: A rapid, high selective ultra-high performance liquid chromatographic electrospray quadrupole- time of flight mass spectrometry (UHPLC-ESI-Q-TOF/MS) method was applied to investigate the pharmacokinetics of benzoic acid, 4-[[(2-hydroxyethyl)amino]carbonyl]-, methyl ester with p-coumaric acid as internal standard (IS) in rats after intravenously and orally dosed.

Results: The pharmacokinetic data of benzoic acid, 4-[[(2-hydroxyethyl)amino]carbonyl]-, methyl ester was analyzed in the two-compartment open model. The main pharmacokinetic parameters were, respectively, 36.474 μg·h/mL, 12.59 μg·h/mL (AUC0→∞), and T1/2α was 0.14 h, 0.359 h; T1/2β was 3.046 h, 5.646 h after intravenous and oral administrations.

Conclusion: Benzoic acid, 4-[[(2-hydroxyethyl)amino] carbonyl]-, methyl ester was rapidly distributed in rat’s plasma with the absolute bioavailability of 34.5%.

Keywords: Portulaca oleracea L., Benzoic acid, 4-[[(2-hydroxyethyl)amino]carbonyl], methyl ester, pharmacokinetics, ultrahigh performance liquid chromatography-electrospray quadrupole-time of flight mass spectrometry, rat.

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Article Details

VOLUME: 16
ISSUE: 5
Year: 2020
Published on: 15 June, 2020
Page: [601 - 607]
Pages: 7
DOI: 10.2174/1573412915666190320154857
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