A Comprehensive Review on the Screening Models for the Pharmacological Assessment of Antiulcer Drugs

Author(s): Abhinav P. Mishra*, Ankit Bajpai, Suresh Chandra

Journal Name: Current Clinical Pharmacology
Continued as Current Reviews in Clinical and Experimental Pharmacology

Volume 14 , Issue 3 , 2019

Graphical Abstract:


Background: Due to inappropriate diet, smoking, alcohol consumption, regular use of drugs like NSAIDs and sedentary lifestyle, one may feel upper abdominal pain which may be the predictor of the gastrointestinal disorder called Peptic Ulcer. When an imbalance occurs between the defensive factor and aggressive factor of the stomach, ulcer formation in the esophageal lining, stomach, or duodenum takes place. This leads to the formation of small sores that cause pain. Another condition that synergizes the abdominal pain is vomiting materials which look like coffee grounds, blood in the stool, black or tarry stools. This pain may increase after lunch or dinner. This problem persists, that often leads to the gastroenterologist's consultation.

Objective: There are many antiulcer screening models present for the determination of antiulcer activity of the drug molecule. The main objective of this study is to find which model is best for the determination of antiulcer activity.

Methods: A literature search was conducted on the databases namely Science direct and PubMed with the help of different keywords such as "Anti-ulcer", "In-vitro models" and "In-vivo models". The search was customized by applying the appropriate filters so as to get the most relevant articles to meet the objective of this review article.

Result: There are different research and review papers based on the antiulcer screening models for the determination of antiulcer activity of new drug molecules.

Conclusion: On the basis of our study, we found some useful models for the antiulcer activity of drugs and suggested that, if we use in-vitro and in-vivo methods together, then we may obtain the most relevant result in our research area.

Keywords: Helicobacter pylori, proton pump inhibitor, cysteamine, histamine, NSAIDs, cushing ulcer.

Kaur A, Singh R, Sharma R, Kumar S. Peptic ulcer: A review on etiology and pathogenesis. Int Res J Pharm 2012; 3(6): 34-8.
Vomero ND, Colpo E. Nutritional care in peptic ulcer. Arq Bras Cir Dig 2014; 27(4): 298-302.
[http://dx.doi.org/10.1590/S0102-67202014000400017] [PMID: 25626944]
Prabhu V, Shivani A. An overview of history, pathogenesis and treatment of perforated peptic ulcer disease with evaluation of prognostic scoring in adults. Ann Med Health Sci Res 2014; 4(1): 22-9.
[http://dx.doi.org/10.4103/2141-9248.126604] [PMID: 24669326]
Lanza FL, Chan FKL, Quigley EMM. Parameters P. Practice. Guidelines for prevention of NSAID-related ulcer complications. Am J Gastroenterol 2009; 104(3): 728-38.
[http://dx.doi.org/10.1038/ajg.2009.115] [PMID: 19240698]
Malfertheiner P, Megraud F, O’Morain C, et al. Current concepts in the management of Helicobacter pylori infection: The Maastricht III Consensus Report. Gut 2007; 56(6): 772-81.
[http://dx.doi.org/10.1136/gut.2006.101634] [PMID: 17170018]
Wolfe MM, Soll AH. The physiology of gastric acid secretion. N Engl J Med 1988; 319(26): 1707-15.
[http://dx.doi.org/10.1056/NEJM198812293192605] [PMID: 3060722]
Waldum HL, Sandvik AK, Syversen U, Brenna E. The Entero-Chromaffin-Like (ECL) cell. Physiological and pathophysiological role. Acta Oncol 1993; 32(2): 141-7.
[http://dx.doi.org/10.3109/02841869309083903] [PMID: 8323755]
Andersson N, Rhedin M, Peteri-Brunbäck B, Andersson K, Cabero JL. Gastrin effects on isolated rat enterochromaffin-like cells following long-term hypergastrinaemia in vivo. Biochim Biophys Acta 1999; 1451(2-3): 297-304.
[http://dx.doi.org/10.1016/S0167-4889(99)00101-9] [PMID: 10556584]
Wolfe MM, Sachs G. Acid suppression: Optimizing therapy for gastroduodenal ulcer healing, gastroesophageal reflux disease, and stress-related erosive syndrome. Gastroenterology 2000; 118(2)(Suppl. 1): S9-S31.
[http://dx.doi.org/10.1016/S0016-5085(00)70004-7] [PMID: 10868896]
Oka K, Seki T, Akatsu T, Wakabayashi T, Inui K, Yoshino J. Clinical study using novel endoscopic system for measuring size of gastrointestinal lesion. World J Gastroenterol 2014; 20(14): 4050-8.
[http://dx.doi.org/10.3748/wjg.v20.i14.4050] [PMID: 24744595]
Adinortey MB, Ansah C, Galyuon I, Nyarko A. In-vivo models used for evaluation of potential antigastroduodenal ulcer agents. Ulcer 2013; 2013: 1-12.
Roskoski R Jr. Guidelines for preparing color figures for everyone including the color blind. Pharmacol Res 2017; 119: 240-1.
[http://dx.doi.org/10.1016/j.phrs.2017.02.005] [PMID: 28189785]
Jia YT, Wei W, Ma B, et al. Activation of p38 MAPK by reactive oxygen species is essential in a rat model of stress-induced gastric mucosal injury. J Immunol 2007; 179(11): 7808-19.
[http://dx.doi.org/10.4049/jimmunol.179.11.7808] [PMID: 18025227]
Bae DK, Park D, Lee SH, et al. Different antiulcer activities of pantoprazole in stress, alcohol and pylorus ligation-induced ulcer models. Lab Anim Res 2011; 27(1): 47-52.
[http://dx.doi.org/10.5625/lar.2011.27.1.47] [PMID: 21826160]
Malfertheiner P, Chan FKL, McColl KEL. Peptic ulcer disease. Lancet 2009; 374(9699): 1449-61.
[http://dx.doi.org/10.1016/S0140-6736(09)60938-7] [PMID: 19683340]
Lee A. Animal models of gastroduodenal ulcer disease. Best Pract Res Clin Gastroenterol 2000; 14(1): 75-96.
[http://dx.doi.org/10.1053/bega.2000.0060] [PMID: 10749090]
Paré WP. Psychological studies of stress ulcer in the rat. Brain Res Bull 1980; 5(Suppl. 1): 73-9.
[http://dx.doi.org/10.1016/0361-9230(80)90308-1] [PMID: 6992948]
Scherer LR, Ed. Peptic Ulcer and Other Conditions of the Stomach. In Pediatric Surgery. Elsevier Inc 2012; pp. 1029-39.
Tanaka M, Kohno Y, Tsuda A, et al. Differential effects of morphine on noradrenaline release in brain regions of stressed and non-stressed rats. Brain Res 1983; 275(1): 105-15.
[http://dx.doi.org/10.1016/0006-8993(83)90422-5] [PMID: 6578864]
Paré WP, Glavin GB. Restraint stress in biomedical research: A review. Neurosci Biobehav Rev 1986; 10(3): 339-70.
[http://dx.doi.org/10.1016/0149-7634(86)90017-5] [PMID: 3095718]
Appelbaum BD, Holtzman SG. Restraint stress enhances morphine-induced analgesia in the rat without changing apparent affinity of receptor. Life Sci 1985; 36(11): 1069-74.
[http://dx.doi.org/10.1016/0024-3205(85)90492-8] [PMID: 2983165]
Parmar NS, Prakash S, Eds. Screening methods in Pharmacology. Oxford, U.K.: Alpha Science International 2011.
Rao AS, Laxmi NB. Pharmacological screeining methods and toxicology. Pharmamed Press 2014; pp. 239-44.
Vogals HG, Ed. Drug Discovery and evaluation: Pharmacological assays. Heidelberg, Germany: Springer-Verlag Berlin Heidelberg 2008.
Itoh YH, Noguchi R. Pre-treatment with mild whole-body heating prevents gastric ulcer induced by restraint and water-immersion stress in rats. Int J Hyperthermia 2000; 16(2): 183-91.
[http://dx.doi.org/10.1080/026567300285376] [PMID: 10763746]
Garrick T, Buack S, Bass P. Gastric motility is a major factor in cold restraint-induced lesion formation in rats. Am J Physiol 1986; 250(2 Pt 1): G191-9.
[PMID: 3953798]
Hamajima E, Sugiyama S, Hoshino H, Goto H, Tsukamoto Y, Ozawa T. Effects of FK506, an immunosuppressive agent, on genesis of water-immersion stress-induced gastric lesions in rats. Dig Dis Sci 1994; 39(4): 713-20.
[http://dx.doi.org/10.1007/BF02087412] [PMID: 7512015]
Landeira-Fernandez J. Analysis of the cold-water restraint procedure in gastric ulceration and body temperature. Physiol Behav 2004; 82(5): 827-33.
[http://dx.doi.org/10.1016/j.physbeh.2004.06.016] [PMID: 15451646]
Shian WM, Sasaki I, Kamiyama Y, Naito H, Matsuno S, Miyazawa T. The role of lipid peroxidation on gastric mucosal lesions induced by water-immersion-restraint stress in rats. Surg Today 2000; 30(1): 49-53.
[http://dx.doi.org/10.1007/PL00010046] [PMID: 10648083]
Harth M, Bondy DC. Indomethacin and acetylsalicylic acid in the treatment of osteoarthritis of the hips and knees. Can Med Assoc J 1969; 101(6): 311-6.
[PMID: 4898391]
Thompson M, Percy JS. Further experience with indomethacin in the treatment of rheumatic disorders. BMJ 1966; 1(5479): 80-3.
[http://dx.doi.org/10.1136/bmj.1.5479.80] [PMID: 4159244]
Regula J, Butruk E, Dekkers CPM, et al. Prevention of NSAID-associated gastrointestinal lesions: A comparison study pantoprazole versus omeprazole. Am J Gastroenterol 2006; 101(8): 1747-55.
[http://dx.doi.org/10.1111/j.1572-0241.2006.00686.x] [PMID: 16817839]
Becker JC, Domschke W, Pohle T. Current approaches to prevent NSAID-induced gastropathy-COX selectivity and beyond. Br J Clin Pharmacol 2004; 58(6): 587-600.
[http://dx.doi.org/10.1111/j.1365-2125.2004.02198.x] [PMID: 15563357]
Morioka N, Kumagai K, Morita K, Kitayama S, Dohi T. Nonsteroidal anti-inflammatory drugs potentiate 1-methyl-4-phenylpyridinium (MPP+)-induced cell death by promoting the intracellular accumulation of MPP+ in PC12 cells. J Pharmacol Exp Ther 2004; 310(2): 800-7.
[http://dx.doi.org/10.1124/jpet.104.065300] [PMID: 15131242]
Maity P, Bindu S, Dey S, et al. Melatonin reduces indomethacin-induced gastric mucosal cell apoptosis by preventing mitochondrial oxidative stress and the activation of mitochondrial pathway of apoptosis. J Pineal Res 2009; 46(3): 314-23.
[http://dx.doi.org/10.1111/j.1600-079X.2009.00663.x] [PMID: 19220725]
Maity P, Bindu S, Dey S, et al. Indomethacin, a non-steroidal anti-inflammatory drug, develops gastropathy by inducing re-active oxygen species-mediated mitochondrial pathology and associated apoptosis in gastric mucosa. J Biol Chem 2009; 284(5): 3058-68.
[http://dx.doi.org/10.1074/jbc.M805329200] [PMID: 19049974]
Pal C, Bindu S, Dey S, et al. Gallic acid prevents nonsteroidal anti-inflammatory drug-induced gastropathy in rat by blocking oxidative stress and apoptosis. Free Radic Biol Med 2010; 49(2): 258-67.
[http://dx.doi.org/10.1016/j.freeradbiomed.2010.04.013] [PMID: 20406680]
Alsarra IA, Ahmed MO, Alanazi FK, Eltahir KE, Alsheikh AM, Neau SH. Influence of cyclodextrin complexation with NSAIDs on NSAID/cold stress-induced gastric ulceration in rats. Int J Med Sci 2010; 7(4): 232-9.
[http://dx.doi.org/10.7150/ijms.7.232] [PMID: 20617127]
Musumba C, Pritchard DM, Pirmohamed M. Review article: Cellular and molecular mechanisms of NSAID-induced peptic ulcers. Aliment Pharmacol Ther 2009; 30(6): 517-31.
[http://dx.doi.org/10.1111/j.1365-2036.2009.04086.x] [PMID: 19575764]
Paré WP, Redei E. Depressive behavior and stress ulcer in Wistar Kyoto rats. J Physiol Paris 1993; 87(4): 229-38.
[http://dx.doi.org/10.1016/0928-4257(93)90010-Q] [PMID: 8136789]
López-Rubalcava C, Lucki I. Strain differences in the behavioral effects of antidepressant drugs in the rat forced swimming test. Neuropsychopharmacology 2000; 22(2): 191-9.
[http://dx.doi.org/10.1016/S0893-133X(99)00100-1] [PMID: 10649831]
Armario A, Gavaldà A, Martí J. Comparison of the behavioural and endocrine response to forced swimming stress in five inbred strains of rats. Psychoneuroendocrinology 1995; 20(8): 879-90.
[http://dx.doi.org/10.1016/0306-4530(95)00018-6] [PMID: 8834094]
Rastogi L, Patnaik GK, Dikshit M. Free radicals and antioxidant status following pylorus ligation induced gastric mucosal injury in rats. Pharmacol Res 1998; 38(2): 125-32.
[http://dx.doi.org/10.1006/phrs.1998.0343] [PMID: 9721600]
Kandhare AD, Raygude KS, Ghosh P, Bodhankar SL. The ameliorative effect of fisetin, a bioflavonoid, on ethanol-induced and pylorus ligation-induced gastric ulcer in rats. Int J of Green Pharm 2011; 5: 236-43.
Khushtar M, Kumar V, Javed K, Bhandari U. Protective effect of ginger oil on aspirin and pylorus ligation-induced gastric ulcer model in rats. Indian J Pharm Sci 2009; 71(5): 554-8.
[http://dx.doi.org/10.4103/0250-474X.58195] [PMID: 20502577]
Brodie DA. The mechanism of gastric hyperacidity produced by pylorus ligation in the rat. Am J Dig Dis 1966; 11(3): 231-41.
[http://dx.doi.org/10.1007/BF02233904] [PMID: 4951374]
Cho CH, Pfeiffer CJ. Gastrointestinal ulceration in the guinea pig in response to dimaprit, histamine, and H1- and H2-blocking agents. Dig Dis Sci 1981; 26(4): 306-11.
[http://dx.doi.org/10.1007/BF01308370] [PMID: 6113104]
Alphin RS, Ward JW. An investigation of antihistaminic activity and gastric ulceration. Eur J Pharmacol 1969; 6(1): 61-6.
[http://dx.doi.org/10.1016/0014-2999(69)90066-1] [PMID: 4389066]
Okabe S, Takeuchi K, Urushidani T, Takagi K. Effects of cimetidine, a histamine H2-receptor antagonist, on various experimental gastric and duodenal ulcers. Am J Dig Dis 1977; 22(8): 677-84.
[http://dx.doi.org/10.1007/BF01078346] [PMID: 18007]
Guslandi M. Effects of ethanol on the gastric mucosa. Dig Dis 1987; 5(1): 21-32.
[http://dx.doi.org/10.1159/000171159] [PMID: 3297432]
Laine L, Weinstein WM. Histology of alcoholic hemorrhagic “gastritis”: A prospective evaluation. Gastroenterology 1988; 94(6): 1254-62.
[http://dx.doi.org/10.1016/0016-5085(88)90661-0] [PMID: 3258836]
Oates PJ, Hakkinen JP. Studies on the mechanism of ethanol-induced gastric damage in rats. Gastroenterology 1988; 94(1): 10-21.
[http://dx.doi.org/10.1016/0016-5085(88)90604-X] [PMID: 3335281]
Guth PH, Paulsen G, Nagata H. Histologic and microcirculatory changes in alcohol-induced gastric lesions in the rat: Effect of prostaglandin cytoprotection. Gastroenterology 1984; 87(5): 1083-90.
[PMID: 6207071]
Glavin GB, Szabo S. Experimental gastric mucosal injury: Laboratory models reveal mechanisms of pathogenesis and new therapeutic strategies. FASEB J 1992; 6(3): 825-31.
[http://dx.doi.org/10.1096/fasebj.6.3.1740232] [PMID: 1740232]
Repetto MG, Llesuy SF. Antioxidant properties of natural compounds used in popular medicine for gastric ulcers. Braz J Med Biol Res 2002; 35(5): 523-34.
[http://dx.doi.org/10.1590/S0100-879X2002000500003] [PMID: 12011936]
Hollander D. Wski Ata, Krause W, Gergely H. Protective effect of sucralfate against alcohol-induced gastric mucosal in-jury in the rat macroscopic, histologic, ultrastructural, and functional time sequence analysis. Gastroenterol 1985; 88(1): 366-74.
[http://dx.doi.org/10.1016/S0016-5085(85)80191-8] [PMID: 3871090]
Mizui T, Doteuchi M. Effect of polyamines on acidified ethanol-induced gastric lesions in rats. Jpn J Pharmacol 1983; 33(5): 939-45.
[http://dx.doi.org/10.1254/jjp.33.939] [PMID: 6580476]
Park S, Hahm KB, Oh TY, Jin JH, Choue R. Preventive effect of the flavonoid, wogonin, against ethanol-induced gastric mucosal damage in rats. Dig Dis Sci 2004; 49(3): 384-94.
[http://dx.doi.org/10.1023/B:DDAS.0000020490.34220.6d] [PMID: 15139485]
Okabe S, Pfeiffer CJ. Chronicity of acetic acid ulcer in the rat stomach. Am J Dig Dis 1972; 17(7): 619-29.
[http://dx.doi.org/10.1007/BF02231748] [PMID: 5032686]
Takagi K, Okabe S, Saziki R. A new method for the production of chronic gastric ulcer in rats and the effect of several drugs on its healing. Jpn J Pharmacol 1969; 19(3): 418-26.
[http://dx.doi.org/10.1254/jjp.19.418] [PMID: 5307474]
Okabe S, Roth JLA, Pfeiffer CJ. A method for experimental, penetrating gastric and duodenal ulcers in rats. Observations on normal healing. Am J Dig Dis 1971; 16(3): 277-84.
[http://dx.doi.org/10.1007/BF02235252] [PMID: 5554507]
Okabe S, Amagase K. An overview of acetic acid ulcer models-the history and state of the art of peptic ulcer research. Biol Pharm Bull 2005; 28(8): 1321-41.
[http://dx.doi.org/10.1248/bpb.28.1321] [PMID: 16079471]
Cheng CL, Guo JS, Luk J, Koo MWL. The healing effects of Centella extract and asiaticoside on acetic acid induced gastric ulcers in rats. Life Sci 2004; 74(18): 2237-49.
[http://dx.doi.org/10.1016/j.lfs.2003.09.055] [PMID: 14987949]
Wang JY, Yamasaki S, Takeuchi K, Okabe S. Delayed healing of acetic acid-induced gastric ulcers in rats by indomethacin. Gastroenterology 1989; 96(2 Pt 1): 393-402.
[http://dx.doi.org/10.1016/0016-5085(89)91563-1] [PMID: 2910759]
Seley H, Szabo S. Experimental model for perforating duode-nal ulcers by cysteamine in the rats. Nature. Int J Sci 1973; 244: 458-9.
Szabo S. Duodenal ulcer disease. Animal model: Cysteamine-induced acute and chronic duodenal ulcer in the rat. Am J Pathol 1978; 93(1): 273-6.
[PMID: 696806]
Cahill MC, Gallagher GT, Szabo S. Cysteamine induces duodenal ulcer in the mouse. Digestion 1986; 34(1): 1-8.
[http://dx.doi.org/10.1159/000199303] [PMID: 3709997]
Adler RS, Gallagher GT, Szabo S. Duodenal ulcerogens cysteamine and propionitrile decrease duodenal neutralization of acid in the rat. Dig Dis Sci 1983; 28(8): 716-23.
[http://dx.doi.org/10.1007/BF01312562] [PMID: 6872804]
Bedekovic V, Mise S, Anic T, et al. Different effect of antiulcer agents on rat cysteamine-induced duodenal ulcer after sialoadenectomy, but not gastrectomy. Eur J Pharmacol 2003; 477(1): 73-80.
[http://dx.doi.org/10.1016/j.ejphar.2003.08.013] [PMID: 14512101]
Minaiyana M, Ghannadi A, Salehic E. Antiulcerogenic effect of Zataria multiflora Boiss. on cysteamine induced duodenal ulcer in rats. Iranian J Pharmaceut Sci 2005; 1(4): 223-9.
Saghaei F, Karimi I, Jouyban A, Samini M. Effects of captopril on the cysteamine-induced duodenal ulcer in the rat. Exp Toxicol Pathol 2012; 64(4): 373-7.
[http://dx.doi.org/10.1016/j.etp.2010.10.001] [PMID: 21036019]
Whittle BJ. Relationship between the prevention of rat gastric erosions and the inhibition of acid secretion by prostaglandins. Eur J Pharmacol 1976; 40(2): 233-9.
[http://dx.doi.org/10.1016/0014-2999(76)90057-1] [PMID: 11107]
Takeuchi K, Furukawa O, Tanaka H, Okabe S. A new model of duodenal ulcers induced in rats by indomethacin plus histamine. Gastroenterology 1986; 90(3): 636-45.
[http://dx.doi.org/10.1016/0016-5085(86)91118-2] [PMID: 3943695]
Levine RA, Schwartzel EH. Effect of indomethacin on basal and histamine stimulated human gastric acid secretion. Gut 1984; 25(7): 718-22.
[http://dx.doi.org/10.1136/gut.25.7.718] [PMID: 6735252]
Takeuchi K, Furukawa O, Okada M, Okabe S. Duodenal ulcer induced by indomethacin plus histamine in the dog. Digestion 1988; 39(4): 230-40.
[http://dx.doi.org/10.1159/000199631] [PMID: 3220176]
Takeuchi K, Okada M, Niida H, Okabe S. Healing process of duodenal ulcers induced by indomethacin plus histamine in rats. Digestion 1989; 42(4): 202-11.
[http://dx.doi.org/10.1159/000199847] [PMID: 2806753]
Wallace JL, McKnight W, Reuter BK, Vergnolle N. NSAID-induced gastric damage in rats: Requirement for inhibition of both cyclooxygenase 1 and 2. Gastroenterology 2000; 119(3): 706-14.
[http://dx.doi.org/10.1053/gast.2000.16510] [PMID: 10982765]
Rainsford KD. The effects of 5-lipoxygenase inhibitors and leukotriene antagonists on the development of gastric lesions induced by nonsteroidal antiinflammatory drugs in mice. Agents Actions 1987; 21(3-4): 316-9.
[http://dx.doi.org/10.1007/BF01966502] [PMID: 3687587]
Graham DY, Agrawal NM, Roth SH. Prevention of NSAID-induced gastric ulcer with misoprostol: Multicentre, double-blind, placebo-controlled trial. Lancet 1988; 2(8623): 1277-80.
[http://dx.doi.org/10.1016/S0140-6736(88)92892-9] [PMID: 2904006]
Ma XJ, Lu GC, Song SW, et al. The features of reserpine-induced gastric mucosal lesions. Acta Pharmacol Sin 2010; 31(8): 938-43.
[http://dx.doi.org/10.1038/aps.2010.74] [PMID: 20686519]
Nedelcu L, Grapa D, Sandor V, Orbai P, Dumitrascu DL. Inhibition of reserpine-induced ulcers by calcitonin in rats. Bull Transil Univer Bras 2011; 4(1): 21-6.
Gupta MB, Tangri KK, Bhargava KP. Mechanism of ulcerogenic activity of reserpine in albino rats. Eur J Pharmacol 1974; 27(2): 269-71.
[http://dx.doi.org/10.1016/0014-2999(74)90159-9] [PMID: 4853131]
Mi T, Ldam A. An overview of in-vivo and in-vitro models that can be used for evaluating anti-gastric ulcer potential of medicinal plants. Austin biol 2016; 1(2): 1-9.
Panda V, Khambat P. Evaluation of antacid activity of Garcinia Indica fruit rind by a modified artificial stomach model. BEPLS 2013; 2(June): 38-42.
Vatier J, Malikova-Sekera E, Vitre MT, Mignon M. An artificial stomach-duodenum model for the in-vitro evaluation of antacids. Aliment Pharmacol Ther 1992; 6(4): 447-58.
[http://dx.doi.org/10.1111/j.1365-2036.1992.tb00558.x] [PMID: 1420737]
Fordtran JS, Morawski SG, Richardson CT. In-vivo and In-vitro evaluation of liquid antacid. N Eng J M 1973; 288(18): 923-8.
Sandhya SK VR, Vinod KR. A Comparative evaluation of In-vitro antacid activity of two Tephrosia species using modified artificial stomach model. Hygeia J D Med 2016; 7(2): 9-17.
Sandhya SK VR, Vinod KR, Rsnakk C. Assessment of In-vitro antacid activity of different root extracts of Tephrosia purpurea (L) Pers by modified artificial stomach model. Asian Pac J Trop Biomed 2012; 2(3): S1487-s1492.
Christensen CB, Soelberg J, Jäger AK. Antacid activity of Laportea aestuans (L.) Chew. J Ethnopharmacol 2015; 171: 1-3.
[http://dx.doi.org/10.1016/j.jep.2015.05.026] [PMID: 26023029]
Houshia OJ, AbuEid M, Zaid O, Zaid M, Al-daqqa N. Assessment of the value of the antacid contents of selected palestinian plants. Am J Chem 2012; 2(6): 322-5.
Roopa G, Bhat RS, Dakshina MS. Formulation and evaluation of an antacid and anti-ulcer suspension containing herbal drugs. Biomed Pharmacol J 2010; 3(1): 1-6.
Gupta PC, Rao CV, Sharma N. Protective effect of standardized extract of Cleome viscosa against experimentally induced gastric lesions in the rat. Pharm Biol 2013; 51(5): 595-600.
[http://dx.doi.org/10.3109/13880209.2012.753918] [PMID: 23350708]
Zhang Q, Huang N, Wang J, et al. The H+/K+-ATPase inhibitory activities of Trametenolic acid B from Trametes lactinea (Berk.) Pat, and its effects on gastric cancer cells. Fitoterapia 2013; 89: 210-7.
[http://dx.doi.org/10.1016/j.fitote.2013.05.021] [PMID: 23742858]
Bardou M, Toubouti Y, Benhaberou-Brun D, Rahme E, Barkun AN, General M. Meta-analysis: Proton-pump inhibition in high-risk patients with acute peptic ulcer bleeding. Aliment Pharmacol Ther 2005; 21(6): 677-86.
[http://dx.doi.org/10.1111/j.1365-2036.2005.02391.x] [PMID: 15771753]
Burci LM, Pereira IT, da Silva LM, et al. Antiulcer and gastric antisecretory effects of dichloromethane fraction and piplartine obtained from fruits of Piper tuberculatum Jacq. in rats. J Ethnopharmacol 2013; 148(1): 165-74.
[http://dx.doi.org/10.1016/j.jep.2013.04.006] [PMID: 23588092]
Takagi K, Okabe S. The effects of drugs on the production and recovery processes of the stress ulcer. Jpn J Pharmacol 1968; 18(1): 9-18.
[http://dx.doi.org/10.1254/jjp.18.9] [PMID: 5302464]
Nwafor PA, Okwuasaba FK, Binda LG. Antidiarrhoeal and antiulcerogenic effects of methanolic extract of Asparagus pubescens root in rats. J Ethnopharmacol 2000; 72(3): 421-7.
[http://dx.doi.org/10.1016/S0378-8741(00)00261-0] [PMID: 10996281]
Desai JK, Goyal RK, Parmar NS. Characterization of dopamine receptor subtypes involved in experimentally induced gastric and duodenal ulcers in rats. J Pharm Pharmacol 1999; 51(2): 187-92.
[http://dx.doi.org/10.1211/0022357991772123] [PMID: 10217318]
Andrade SF, Antoniolli D, Comunello E, Cardoso LGV, Carvalho JCT, Bastos JK. Antiulcerogenic activity of crude extract, fractions and populnoic acid isolated from Austroplenckia populnea (Celastraceae). Z Natforsch C J Biosci 2006; 61(5-6): 329-33.
[http://dx.doi.org/10.1515/znc-2006-5-605] [PMID: 16869488]
Ganguly AK. A method for quantitative assessment of experimentally produced ulcers in the stomach of albino rats. Experientia 1969; 25(11): 1224.
[http://dx.doi.org/10.1007/BF01900290] [PMID: 5357845]
Morini G, Grandi D. Methods to measure gastric mucosal lesions in rats. Curr Protoc Toxicol 2010 February: 21.2.1-21.2.15.

open access plus

Rights & PermissionsPrintExport Cite as

Article Details

Year: 2019
Published on: 12 March, 2019
Page: [175 - 196]
Pages: 22
DOI: 10.2174/1574884714666190312143846

Article Metrics

PDF: 28
PRC: 1