Analysis of the Essential Oils of Five Artemisia Species and Evaluation of their Cytotoxic and Proapoptotic Effects

Author(s): Zahra Tayarani-Najaran, Maryam Akaberi, Bahareh Hassanzadeh, Nazila Shirazi, Javad Asili, Hadi Al-Najjar, Amirhossein Sahebkar, Seyed Ahmad Emami*

Journal Name: Mini-Reviews in Medicinal Chemistry

Volume 19 , Issue 11 , 2019

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Graphical Abstract:


Background: In this study, the essential oil composition and cytotoxic activities of five Artemisia species were determined.

Methods: The collected plants were water-distilled separately to obtain oils which were then subjected to gas chromatography (GC) and gas chromatography/mass spectrometry GC/MS analyses to identify their compositions. Cancer cells were exposed to different concentrations of samples and cell viability was measured using AlamarBlue® assay. Apoptotic cells were analyzed by propidium iodide (PI) staining and flow cytometry.

Results & Conclusion: To study the amount of pro-apoptotic proteins and the apoptosis mechanism, Western blot method was used. Although all samples were cytotoxic at the highest concentration, the oil of A. kulbadica showed the strongest activity among other plants. Carvacrol (IC50 21.11 μg/ml) had the most cytotoxic effects among other components. Carvacrol, 1,8-cineole and 4-terpineole caused an increase in the amount of Bax protein and cleaved peroxisome proliferator-activated receptors (PPAR) and caspase proteins in DU 145 cells.

Keywords: Artemisia, Asteraceae, essential oil, chemical composition, cytotoxic, apoptosis.

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Article Details

Year: 2019
Published on: 11 March, 2019
Page: [902 - 912]
Pages: 11
DOI: 10.2174/1389557519666190311155021
Price: $65

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