Recent Applications of High Performance Thin Layer Chromatography and Derivative Spectrophotometry in Pharmaceutical Analysis

Author(s): Marcin Gackowski*, Marcin Koba, Katarzyna Mądra-Gackowska, Piotr Kośliński, Stefan Kruszewski

Journal Name: Current Pharmaceutical Analysis

Volume 16 , Issue 6 , 2020


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Graphical Abstract:


Abstract:

At present, no one can imagine drug development, marketing and post-marketing without rigorous quality control at each stage. Only modern, selective, accurate and precise analytical methods for determination of active compounds, their degradation products and stability studies are able to assure the appropriate amount and purity of drugs administered every day to millions of patients all over the world. For routine control of drugs simple, economic, rapid and reliable methods are desirable. The major focus of current scrutiny is placed on high-performance thin layer chromatography and derivative spectrophotometry methods, which fulfill routine drug estimation’s expectations [1-4]. The present paper reveals state-of-the-art and possible applications of those methods in pharmaceutical analysis between 2010 and 2018. The review shows advantages of high-performance thin layer chromatography and derivative spectrophotometry, including accuracy and precision comparable to more expensive and time-consuming methods as well as additional fields of possible applications, which contribute to resolving many analytical problems in everyday laboratory practice.

Keywords: Analytical methods, high performance thin layer chromatography, derivative spectrophotometry, drug analysis, pharmaceutical formulations, pharmaceutical analysis.

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