Glucose Lowering Activity of Aqueous Ammodaucus leucotrichus Extract in Diabetic Rats

Author(s): Fadwa El-Ouady, Mohamed Eddouks*

Journal Name: Cardiovascular & Hematological Disorders-Drug Targets
(Formerly Current Drug Targets - Cardiovascular & Hematological Disorders)

Volume 20 , Issue 2 , 2020

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Abstract:

Background: Ammodaucus leucotrichus (Apiaceae) is a medicinal plant widely used by the Moroccan population to treat various diseases including diabetes.

Aims: The aim of this study was to evaluate the antidiabetic effect of the aqueous extract prepared from the fruits of this medicinal plant. Normal and streptozotocin-induced diabetic rats were treated orally by the extract of Ammodaucus leucotrichus (A. leucotrichus) at a dose of 10 mg/kg.

Methods: The blood glucose levels were determined during 6 hours and 15 days during this treatment. In addition the oral glucose tolerance test as well as the analysis of histopathological changes of liver was performed. The antioxidant activity of aqueous A. Leucotrichus extract was also valuated by the method of trapping of free radical 2,2-diphenyl-1 picrylhydrazyl (DPPH).

Results: A. Leucotrichus fruit aqueous extract (ALFAE) reduced the blood glucose levels in both normal and diabetic rats. In addition, a remarkable influence on glucose tolerance was also noticed after ALFAE treatment. Moreover, ALFAE was able to exhibit a beneficial effect on histologlogical structure of liver. Finally a potential antioxidant effect has been shown in vitro.

Conclusion: In conclusion, this study demonstrates the antihyperglycemic and hypoglycemic effects of A. Leucotrichus in rats supporting then its traditional use for the management of diabetes.

Keywords: Antidiabetic, Ammodaucus leucotrichus, histopathology, antioxidant activity, diabetes, lithiasis.

[1]
Bnouham, M.; Ziyyat, A.; Mekhfi, H.; Tahri, A.; Legssyer, A. Medicinal plants with potential antidiabetic activity - A review of ten years of herbal medicine research (1990-2000). Int. J. Diabetes Metab., 2006, 14, 1-25.
[http://dx.doi.org/10.1159/000497588]
[2]
Tiwari, A.K.; Madhusudana, R. Diabetes mellitus and multiple therapeutic approaches of phytochemicals: Present status and future prospects. Curr. Sci., 2002, 83, 30-38.
[3]
Spasov, A.A.; Petrov, V.I.; Chepliaeva, N.I.; Lenskaia, K.V. [Fundamental bases of search of medicines for therapy of a diabetes mellitus type 2]. Vestn. Akad. Med. Nauk SSSR, 2013, 2(2), 43-49.
[http://dx.doi.org/10.15690/vramn.v68i2.548] [PMID: 23819328]
[4]
Maiese, K.; Chong, Z.Z.; Shang, Y.C.; Wang, S. Novel directions for diabetes mellitus drug discovery. Expert Opin. Drug Discov., 2013, 8(1), 35-48.
[http://dx.doi.org/10.1517/17460441.2013.736485] [PMID: 23092114]
[5]
Marles, R.J.; Farnsworth, N.R. Antidiabetic plants and their active constituents. Phytomedicine, 1995, 2(2), 137-189.
[http://dx.doi.org/10.1016/S0944-7113(11)80059-0] [PMID: 23196156]
[6]
WHO. Expert Committee on Diabetes Mellitus Second Report,Technical report series 464, WHO, Geneva, Switzerland,, 1980, 61.
[7]
Beltran Tejera, E. Un nuevo Taxon del género Ammodaucus Cosson & Durieu (Apiaceae) en el Archipiélago Canario. Candollea, 1983, 38(1), 131.
[8]
Velasco-Negueruela, A.; Pérez-Alonso, M.J.; Pérez de Paz, P.L.; Palá-Paúl, J.; Sanz, J. Analysis by gas chromatography-mass spectrometry of the volatiles from the fruits of Ammodaucus leucotrichus subsp. leucotrichus and subsp. nanocarpus grown in North Africa and the Canary Islands, respectively. J. Chromatogr. A, 2006, 1108(2), 273-275.
[http://dx.doi.org/10.1016/j.chroma.2006.01.031] [PMID: 16472529]
[9]
Benhouhou, S. Ammodaucus leucotrichus Coss. & Dur. In “A Guide to Me-dicinal Plants in North Africa (Málaga, Spain) In: Ed by: Interna-tional Union for Conservationof Nature and Centre for Medi-terranean Cooperation,; Málaga, Spain. , 2005; pp. 31-32.
[10]
El-Mansouri, L.; Ennabili, A.; Bousta, D. Socioeconomic interest and valorization of medicinal plants from the Rissani oasis (SE of Morocco). Bol. Latinoamer. Caribe. Plantas. Med. Arom., 2011, 10, 30-45.
[11]
Hmamouchi, M. Plantes alimentaires, aromatiques,condimentaires, médicinales et toxiques au Maroc. In: Identification of wild foodand nn-food plants of the Mediter-ranean region; Heywood,VH .; Skoula, M., Eds.; Chania: CIHEAM. , 1997; pp. 89-108.
[12]
Nassif, F.; Tanji, A. Gathered food plants in Morocco: The long forgotten species in ethnobotanical research. Life Sci., 2013, 3, 17-54.
[13]
Beghalia, M.; Ghalem, S.; Allali, H.; Belouatek, A.; Marouf, A. Inhibition of calcium oxalate monohydrate crystal growth using. J. Med. Plants Res., 2008, 2(3), 66-70.
[14]
Gherraf, N.; Zellagui, A.; Kabouche, A.; Lahoual, M.; Salhi, R.; Rhouati, S. Chemical constituents and antimicrobial activity of essential oils of Ammodaucus leucotricus. Arab. J. Chem., 2013, 1, 1-3.
[15]
Louail, Z.; Kameli, A.; Benabdelkader, T.; Bouti, K.; Hamza, K.; Krimat, S. Antimicrobial and antioxidant activity of essential oil of Ammodaucus leucotrichus Coss. & Dur. Seeds. J. Mater. Environ. Sci., 2016, 7, 2328-2334.
[16]
Hebi, M.; Farid, O.; Ajebli, M.; Eddouks, M. Potent antihyperglycemic and hypoglycemic effect of Tamarix articulata Vahl. in normal and streptozotocin-induced diabetic rats. Biomed. Pharmacother., 2017, 87, 230-239.
[http://dx.doi.org/10.1016/j.biopha.2016.12.111] [PMID: 28061406]
[17]
Blois, M.S. Antioxidant determinations by the use of a stable free radical. Nature, 1958, 181, 1199-1200.
[http://dx.doi.org/10.1038/1811199a0]
[18]
Hebi, M.; Eddouks, M. Évaluation de l’activité antioxydante de Stevia rebaudiana. Phytotherapie, 2016, 14(1), 17-22.
[http://dx.doi.org/10.1007/s10298-015-0999-y]
[19]
Mohamed, G.; Lahcen, Z.; Allal, D. Usage des plantes médicinales dans le traitement du diabète au Sahara marocain (Tan- Tan). J. Anim. Plant Sci., 2013, 17(1), 2388-2411.
[20]
Bouallala, M.; Bradai, L.; Abid, M. Diversité et utilisation des plantes spontanées du Sahara septentrional algérien dans la pharmacopée saharienne. Cas de la région du Souf. , Revue ElWahat pour les recherches et les études, 2014, 6, 18-26.
[21]
Orch, H.; Allal, D. Etude ethnobotanique des plantes médicinales utilisées dans le traitement du diabète, et des maladies cardiaques dans la région d’Izaréne (Nord du Maroc). J. Appl. Biosci., 2014, 86, 7640-7956.
[22]
Alia, T.; Esnault, M. Ouled, el.; hadj, K. An ethnopharmacological survey of plants used in traditional diabetes treatment in south- eastern Algeria (Ouargla province). J. Arid Environ., 2016, 127, 82-92.
[http://dx.doi.org/10.1016/j.jaridenv.2015.11.005]
[23]
Kemassi, A.; Darem, S.; Cherif, R.; Boual, Z.; Sadine, S.E.; Aggoune, M.S.; Ould El Hadj, K.A.; Ould Elhadj, M.D. Recherche et identification de quelques plantes médicinales à caractère hypoglycémiant de la pharmacopée traditionnelle des communautés de la vallée du M’Zab (Sahara septentrional Est Algérien). J. Adv. Res. Sci. Technol, 2014, 1(1), 1-5.
[24]
Benkhnigue, O.; Ben Akka, F.; Salhi, S.; Fadli, M.; Douira, A.; Zidane, L. Catalogue des plantes médicinales utilisées dans le traitement du diabète dans la région d’Al Haouz-Rhamna (Maroc). J. Anim. Plant Sci., 2014, 23(1), 3539-3568.
[25]
Ceriello, A. Postprandial hyperglycemia and diabetes complications: is it time to treat? Diabetes, 2005, 54(1), 1-7.
[http://dx.doi.org/10.2337/diabetes.54.1.1] [PMID: 15616004]
[26]
Pham-Huy, L.A.; He, H.; Pham-Huy, C. Free radicals, antioxidants in disease and health. Int. J. Biomed. Sci., 2008, 4(2), 89-96.
[PMID: 23675073]
[27]
Oberley, L.W. Free radicals and diabetes. Free Radic. Biol. Med., 1988, 5(2), 113-124.
[http://dx.doi.org/10.1016/0891-5849(88)90036-6] [PMID: 3075947]
[28]
Lipinski, B. Pathophysiology of oxidative stress in diabetes mellitus. J. Diabetes Complications, 2001, 15(4), 203-210.
[http://dx.doi.org/10.1016/S1056-8727(01)00143-X] [PMID: 11457673]
[29]
Maritim, A.C.; Sanders, R.A.; Watkins, J.B., III Diabetes, oxidative stress, and antioxidants: a review. J. Biochem. Mol. Toxicol., 2003, 17(1), 24-38.
[http://dx.doi.org/10.1002/jbt.10058] [PMID: 12616644]


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VOLUME: 20
ISSUE: 2
Year: 2020
Page: [152 - 159]
Pages: 8
DOI: 10.2174/1871529X19666190222182312
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