Background: Herbal medicines have been used in the treatment of liver diseases
for a long time. The current study was elaborated to evaluate in vitro and in vivo antioxidant
and anti-inflammatory effects of Lotus corniculatus (L. corniculatus) butanolic
Methods: The in vitro antioxidant and anti-inflammatory properties of L. corniculatus
were investigated by employing DPPH radical scavenging, H2O2 scavenging and BSA denaturation
assays. In vivo antioxidant and anti-inflammatory effects of L. corniculatus were
evaluated against paracetamol (APAP)-induced hepatitis in rats. L.corniculatus at doses of
100 and 200 mg/kg was administered orally once daily for seven days. Serum transaminases
(AST and ALT) and lactate dehydrogenase (LDH), total bilirubin levels, liver
malondialdehyde (MDA), reduced glutathione (GSH), glutathione S- transferase (GST)
and superoxide dismutase (SOD) levels and inflammatory markers, such as serum Creactive
protein (CRP), circulating and liver myeloperoxidase (MPO) levels were investigated.
Further histopathological analysis of the liver sections was performed to support the
effectiveness of L. corniculatus.
Results: L. corniculatus exhibited strong antioxidant and anti-inflammatory effects in
vitro. In the in vivo study, our findings demonstrate that L. corniculatus (100 and 200
mg/kg) administration led to an amelioration of APAP effects on liver histology, liver
functions parameters (AST, ALT, LDH, and total bilirubin levels) and liver oxidative
stress markers (MDA, GSH, GST and SOD levels). Furthermore, serum CRP, circulating
MPO and liver MPO levels were declined by both doses of L. corniculatus extract. The
best benefits were observed with 200 mg/kg of L. corniculatus extract.
Conclusion: Antioxidant and anti-inflammatory effects of L. corniculatus extract may be
due to the presence of active components.