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Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry

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ISSN (Print): 1871-5230
ISSN (Online): 1875-614X

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Research Article

Anti-inflammatory Effect of Woodfordia fructicosa Leaves Ethanolic Extract on Adjuvant and Carragenan Treated Rats

Author(s): Hem Raj*, Avneet Gupta and Neeraj Upmanyu

Volume 19, Issue 2, 2020

Page: [103 - 112] Pages: 10

DOI: 10.2174/1871523018666190222120127

Abstract

Background: Woodfordia fructicosa is used traditionally for the treatment of inflammation associated with arthritis.

Methods: In the present study, the anti-inflammatory activity of W. fructicosa (WFE) leaves ethanolic extract was assessed in Sprague Dawley rats by giving 200 mg/kg dose orally. Inflammation was studied by using carrageenan induced paw edema, Freund’s adjuvant (FA) and monosodium iodo acetate (MIA) induced arthritis as animal models. Serum tumor necrosis factor-alpha (TNF-α) was estimated in blood sample of animals treated with FA. The one way ANOVA followed by Bonferroni’s test was used for statistical analysis.

Results: WFE significantly decreased (P<0.05, P<0.001) paw thickness in carrageenan induced paw edema and FA induced arthritis. The significant decrease in knee diameter (P<0.001) in MIA induced arthritis as well as inhibitory effect (P<0.001) on elevated TNF- α was observed.

Conclusion: These results showed that the WFEexerted an inhibitory effect on TNF-α and carrageenan paw edema which may justify its traditional use in inflammatory conditions. Thus, the study shows that leaves of W. fruticose afford anti-inflammatory activity by preventing the inflammation in different animal models.

Keywords: Arthritis, carrageenan, ethanolic extract, freund's adjuvant induced, inflammation, monosodium iodo acetate, paw edema, tumor necrosis factor, Woodfordia fructicosa.

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Graphical Abstract

[1]
Kirtikar, K.R.; Basu, B.D. Indian Medicinal Plants. Parts 1-3; L.M. Basu: Allahabad, India, 1935.
[2]
Vashist, H.; Sharma, D. Ethnobotanical survey of Gharsi village hills and its allied area of District Solan. IJPPS, 2013, 5, 848-850.
[3]
Srivastava, T.N.; Rajasekharan, S.; Badola, D.P.; Shah, D.C. An index of the available medicinal plants, used in Indian system of medicine from Jammu and Kashmir state. Anc. Sci. Life, 1986, 6(1), 49-63.
[PMID: 22557549]
[4]
Chopra, R.N.; Nayar, S.L.; Chopra, I.C. Glossary of Indian Medicinal Plants; Council of Scientific & Industrial Research: Delhi, India, 1956, p. 52.
[5]
Chopra, R.N. Hemraj.; Gupta, A.; Thakur, A; Upmanyu, N. Hydrodistillation of Stephania glabra tuber and Woodfordia fruticosa leaves. Asian J. Pharm. Clin. Res., 2012, 5, 105-107.
[6]
Das, P.K.; Goswami, S.; Chinniah, A.; Panda, N.; Banerjee, S.; Sahu, N.P.; Achari, B. Woodfordia fruticosa: traditional uses and recent findings. 2007, 110(2), 189-199.
[http://dx.doi.org/10.1016/j.jep.2006.12.029] [PMID: 17276634]
[7]
de Lange-Brokaar, B.J.E.; Ioan-Facsinay, A.; van Osch, G.J.; Zuurmond, A.M.; Schoones, J.; Toes, R.E.; Huizinga, T.W.; Kloppenburg, M. Synovial inflammation, immune cells and their cytokines in osteoarthritis: a review. Osteoarthr. Cartil., 2012, 20(12), 1484-1499.
[http://dx.doi.org/10.1016/j.joca.2012.08.027]
[8]
Vashist, H.; Gupta, A.; Upmanyu, N. Role of cytokine in phathogenesis of rheumatoid arthritis- A review. JMPAS, 2016, 2016, 233-251.
[9]
Verma, N.; Amresh, G.; Sahu, P.K.; Mishra, N.; Rao, C.V.; Singh, A.P. Anti-inflammatory and antinoceceptive activity of hydroethanolic extract of Woodfordia fruticosa Kurz flowers Der . Pharm. Sin., 2012, 3, 289-294.
[10]
Mahaveer, G. Bronchoprotective, bronchodialatory and antiinflammatory activity of ethanolic extract of Woodfordia fruticosa (Kurz) flowers. IJPE, 2012, 46, 161-178.
[11]
Baravalia, Y.; Vaghasiya, Y.; Chanda, S. Brine shrimp cytotoxicity, anti-inflammatory and alagesic property of Woodfordia fruticosa Kurz. Iran. J. Pharm. Res., 2012, 11(3), 851-861.
[PMID: 24250512]
[12]
Paterson, H.M.; Murphy, T.J.; Purcell, E.J.; Shelley, O.; Kriynovich, S.J.; Lien, E.; Mannick, J.A.; Lederer, J.A. Injury primes the innate immune system for enhanced Toll-like receptor reactivity. J. Immunol., 2003, 171, 1473-1483.
[13]
Chen, Y.; Yang, L.; Lee, T.J.F. Oroxylin A inhibition of lipopolysaccharide-induced iNOS and COX-2 gene expression via suppression of nuclear factor-kB activation. Biochem. Pharmacol., 2000, 59, 1445-1457.
[14]
Winter, C.A.; Risley, E.A.; Nuss, G.W. Carrageenin-induced edema in hind paw of the rat as an assay for antiiflammatory drugs. Proc. Soc. Exp. Biol and Med., 1962, 111, 544-547.
[15]
Wise, L.E.; Cannavacciulo, R.; Cravatt, B.F.; Martin, B.F.; Lichtman, A.H. Wise evaluation of fatty acid amides in the carrageenan-induced paw edema model. Neuropharmacology, 2007, 54(1), 1-8.
[16]
Cravatt, B.F.; Saghatelian, A.; Hawkins, E.G.; Clement, A.B.; Bracey, M.H.; Lichtman, A.H. Functional disassociation of the central and peripheral fatty acid amide signaling systems. Proc. Natl. Acad. Sci. USA, 2004, 101, 10821-10826.
[http://dx.doi.org/10.1073/pnas.0401292101]
[17]
Umapathy, S.; Balasubramanian, V.; Mariamichael, A. Evaluation of complete freund’s adjuvant arthritis in a wistar rat model. Z. Rheumatol., 2013, 4, 1-7.
[18]
Newbould, B.B. Chemotherapy of arthritis induced in rats by mycobacterial adjuvant. British. J. Pharmacol. Chemother., 1963, 21, 127-136.
[http://dx.doi.org/10.1111/j.1476-5381.1963.tb01508.x]
[19]
Kilimozhi, D.; Parthasarathy, V.; Amuthavalli, N. Effect of Clerodendrum phlomidis on adjuvant induced arthritis in rats- A radiographic densitometric analysis. Int. J. Pharm. Tech. Res., 2009, 1, 1434-1441.
[20]
Bar-Yehuda, S.; Rath-Wolfson, L.; Del Valle, L.; Ochaion, A.; Cohen, S.; Patoka, R.; Zozulya, G.; Barer, F.; Atar, E.; Piña-Oviedo, S.; Perez-Liz, G.; Castel, D.; Fishman, P. Induction of an antiinflammatory effect and prevention of cartilage damage in rat knee osteoarthritis by CF101 treatment. Arthritis Rheum., 2009, 60, 3061-3071.
[21]
Verma, N.; Amresh, G.; Sahu, P.K.; Mishra, N.; Rao, Ch.V.; Singh, A.P. Anti-inflammatory and antinociceptive activity of hydroethanolic extract of Woodfordia fruticosa Kurz flowers. Der. Pharm. Sin., 2012, 3, 289-294.
[22]
Crunkhorn, P.; Meacock, S.C. Mediators of the inflammation induced in the rat paw by carrageenin. Br. J. Pharmacol., 1971, 42, 392-402.
[23]
Vinegar, R.; Schreiber, W.; Hugo, R. Biphasic development of carrageenin edema in rats. J. Pharmacol. Exp. Ther., 1969, 166(1), 96-103.
[PMID: 5776026]
[24]
Di Rosa, M.; Giroud, J.P.; Willoughby, D.A. Studies of the mediators of acute inflammatory response induced in rats in different sites by carrageenan and turpine. J. Pathol., 1971, 101, 15-29.
[25]
Brooks, P.M.; Day, R.O. Nonsteroidal antiinflammatory drugs--differences and similarities. New. Engl. J. Med., 1991, 324, 1716-1725.
[26]
Wills, L.; Montagazza, P.; Horton, E.W. Release of histamin, kinin and prostaglandins during carrageenin induced inflammation of the rats. In: Prostaglandins, Peptides and Amins; Academic Press: London, UK, 1969, pp. 31-48.
[27]
Pearson, C.M. Development of arthritis, periarthritis and periostitis in rats given adjuvants. Proc. Soc. Exp. Biol. Med., 1956, 91, 95-100.
[http://dx.doi.org/10.3181/00379727-91-22179]
[28]
Bendele, A. Animal models of rheumatoid arthritis. J. Musculoskelet. Neuronal Interact., 2001, 1(4), 377-385.
[PMID: 15758488]
[29]
Feldmann, M.; Maini, R.N. Anti-TNF alpha therapy of rheumatoid arthritis: what have we learned? Annu. Rev. Immunol., 2001, 19, 163-196.
[30]
Feldmann, M.; Brennan, F.M.; Maini, R.N. Role of cytokines in rheumatoid arthritis. Annu. Rev. Immunol., 1996, 14, 397-440.
[http://dx.doi.org/10.1146/annurev.immunol.14.1.397]
[31]
Beyreuther, B.; Callizot, N.; Stöhr, T. Antinociceptive efficacy of lacosamide in the monosodium iodoacetate rat model for osteoarthritis pain. Arthritis Res. Ther., 2007, 9, 14.
[32]
Lee, Y.; Pai, M.; Brederson, J.D.; Wilcox, D.; Hsieh, G.; Jarvis, M.F.; Bitner, R.S. Monosodium iodoacetate-induced joint pain is associated with increased phosphorylation of mitogen activated protein kinases in the rat spinal cord. Mol. Pain, 2011, 7, 39.
[33]
Gurău, F.; Baldoni, S.; Prattichizzo, F.; Espinosa, E.; Amenta, F.; Procopio, A.D.; Albertini, M.C.; Bonafè, M.; Olivieri, F. Anti-senescence compounds: a potential nutraceutical approach to healthy aging; Aging Res. Rev, 2018, pp. 46-14.
[http://dx.doi.org/10.1016/j.arr.2018.05.001]
[34]
Piwowarski, J.P.; Granica, S.; Zwierzyńska, M.; Stefańska, J.; Schopohl, P.; Melzig, M.F.; Kiss, A.K. Role of human gut microbiota metabolism in the anti-inflammatory effect of traditionally used ellagitannins-rich plant materials. J. Ethnopharmacol., 2014, 155, 801-809.

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