Title:Advanced High-Coverage Targeted Metabolomics Method (SWATHtoMRM) for Exploring the Relationship of Follicular Fluid Components with Age
VOLUME: 16 ISSUE: 3
Author(s):Jingyan Song, Tianqi Wang, Jiayin Guo, Ying Guo, Xiaoming Wang, Yi Yang, Kaiyue Xu, Yuanhong Sa, Lihua Yuan, Huaying Jiang and Zhengao Sun*
Affiliation:Reproductive and Genetic Center of Integrated Traditional and Western Medicine, the Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, Department of Gynecology and Obstetrics of Traditional Chinese Medicine, the First Clinical College, Shandong University of Traditional Chinese Medicine, Jinan 250014, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, Reproductive and Genetic Center of Integrated Traditional and Western Medicine, the Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, Department of Gynecology and Obstetrics of Traditional Chinese Medicine, the First Clinical College, Shandong University of Traditional Chinese Medicine, Jinan 250014, Department of Gynecology and Obstetrics of Traditional Chinese Medicine, the First Clinical College, Shandong University of Traditional Chinese Medicine, Jinan 250014, Department of Gynecology and Obstetrics of Traditional Chinese Medicine, the First Clinical College, Shandong University of Traditional Chinese Medicine, Jinan 250014, Department of Gynecology and Obstetrics of Traditional Chinese Medicine, the First Clinical College, Shandong University of Traditional Chinese Medicine, Jinan 250014, Department of Gynecology and Obstetrics of Traditional Chinese Medicine, the First Clinical College, Shandong University of Traditional Chinese Medicine, Jinan 250014, Department of Gynecology and Obstetrics of Traditional Chinese Medicine, the First Clinical College, Shandong University of Traditional Chinese Medicine, Jinan 250014, Reproductive and Genetic Center of Integrated Traditional and Western Medicine, the Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011
Keywords:Follicular fluid, metabolomics, age, reproductive function, SWATH to MRM, UPLC-QTOF.
Abstract:
Background: The complexity of follicular fluid metabolome presents a huge challenge for
qualitative and quantitative metabolite profiling and discovery of the comprehensive biomarkers.
Objective: In order to address this challenge, novel SWATHtoMRM metabolomics method was used
for providing broad coverage and excellent quantitative capability to discover the human follicular fluid
metabolites related to age and evaluate their relationship with pregnancy outcome and oocyte senescence.
Methods: The patients were divided into four groups according to age, including group A (28 cases, 21-
27 years old), group B (42 cases, 28-34 years old), group C (31 cases, 35-41 years old), and group D (24
cases, 42-48 years old). Follicular fluid samples from 125 IVF patients were analyzed. The differential
ions among the four groups were identified by principal components analysis according to accurate
mass, isotope ratio, and tandem mass spectroscopic spectra. Then, the differential metabolic pathways
were further identified by a KEGG cluster analysis.
Results: A total of 18 metabolites in the follicular fluid differed among the four groups, including
amino acids, lipids, hormones, and vitamins. A total of 15 metabolites, including 6-oxohexanoate,
phenylalanine, proline, hexadecanoic acid, linoleate, arachidonate, oleic acid, docosahexaenoic acid,
LysoPC(16:1), LysoPC(20:5), LysoPC (20:3), 25-hydroxyvitamin D3, 5-dehydroepisterol, 27-
hydroxycholesterol, and 5beta-cholestane-3alpha,7alpha,12alpha,23,25-pentol, were down-regulated
with age and 3 metabolites, including LysoPC(18:3), LysoPC(18:1), and 13,14-dihydroretinol, were upregulated
with age.
Conclusion: Our study provides useful information for revealing the relationship between age and female
reproductive capability.
