Identification and Quantification of Antioxidant Compounds in Clarified Cashew Apple Juice ‘Cajuína’

Author(s): Rayssa G.L. Porto-Luz, Amanda J.B. de Moura, Bibiana da Silva, Roseane Fett, Marcos A. da Mota Araújo, Regilda S. dos Reis Moreira-Araújo*

Journal Name: Current Nutrition & Food Science

Volume 16 , Issue 4 , 2020


DOI : 10.2174/1573401315666190206124545

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Graphical Abstract:


Abstract:

Background: Cajuína is obtained through clarification of cashew apple juice (Anacardium occidentale L.), a non-diluted and non-fermented beverage, which is prepared from the edible part of the pseudo-fruit.

Objective: This study sought to identify and quantify phenolic compounds, determine vitamin C content, total flavonoid content, and the antioxidant activity of two commercial brands of clarified cashew apple juice (cajuína) produced in Piauí, Brazil.

Methods: Spectrophotometric and high performance liquid chromatography were used.

Results: A high vitamin C content was found mainly in brand A. A high total phenolic content was observed in brand A, and there was no significant difference in flavonoid content between the two brands. Cajuína had high antioxidant activity, which was confirmed by two methods. The highest value was found for brand A by the DPPH method.

Conclusion: We concluded that this clarified cashew apple juice has a high antioxidant compound content, and it is a functional food.

Keywords: Anacardium occidentale L., antioxidant activity, bioactive compounds, clarification of cashew apple juice, identification of phenolic compounds, vitamin C.

[1]
Assunção RB, Mercadante AZ. Carotenoids and ascorbic acid from cashew apple (Anacardium occidentale L.): Variety and geographic effects. Food Chem 2003; 81: 495-502.
[http://dx.doi.org/10.1016/S0308-8146(02)00477-6]
[2]
Petinari RA, Tarsitano MAA. Cashew (Anacardium occidentale L.) commercialization in Northwest of São Paulo state. Rev Bras Frutic 2002; 24: 700-2.
[http://dx.doi.org/10.1590/S0100-29452002000300033]
[3]
IBGE Levantamento Sistemático da Produção Agrícola. (LPSA): Pesquisa Mensal de Previsão e Acompanhamento das Safras Agrícolas no Ano Civil. Rio de Janeiro 2016; 29(12): 1-82.
[4]
Paiva FFA, Garrutti DS, Silva Neto RM. Aproveitamento industrial do caju. Fortaleza: Embrapa Agroindústria Tropical 2000; p. 85.
[5]
BRASIL Ministério da Agricultura,. Pecuária e Abastecimento Instrução Normativa (2000) Art1º - Aprovar o Regulamento técnico geral para fixação dos padrões de identidade e qualidade para polpa de fruta conforme consta do Anexo I desta Instrução Normativa e Art 2º - Regulamento técnico de identidade e qualidade para caju clarificado ou cajuína conforme consta do Anexo II desta Instrução Normativa Brasília, DF. 2000.
[6]
SEBRAE; Relatório de Gestão do exercício de 2016 apresentado aos órgãos de controle interno e externo e à sociedade como prestação de contas anual (2016). IN TCU nº 63/2010, da DN TCU 154/2016, da Portaria TCU 59/2017; https://m.sebrae.com.br/ Sebrae/Portal%20Sebrae/UFs/PI/Anexos/RELAT%C3%93RIO_ GEST%C3%83O_2016_SEBRAE_PIAU%C3%8D.pdfAcesso em 18 dec, 2018.
[7]
de Abreu FP, Dornier M, Dionisio AP, Carail M, Caris-Veyrat C, Dhuique-Mayer C. Cashew apple (Anacardium occidentale L.) extract from by-product of juice processing: a focus on carotenoids. Food Chem 2013; 138(1): 25-31.
[http://dx.doi.org/10.1016/j.foodchem.2012.10.028] [PMID: 23265451]
[8]
Bataglion GA, da Silva FMA, Eberlin MN, Koolen HHF. Simultaneous quantification of phenolic compounds in buriti fruit (Mauritia flexuosa L.f.) by ultra-performance liquid chromatography coupled to tandem mass spectrometry. Food Res Int 2014; 66: 396-400.
[http://dx.doi.org/10.1016/j.foodres.2014.09.035]
[9]
de Lima AC, Soares DJ, da Silva LM, de Figueiredo RW, de Sousa PH, de Abreu Menezes E. In vitro bioaccessibility of copper, iron, zinc and antioxidant compounds of whole cashew apple juice and cashew apple fibre (Anacardium occidentale L.) following simulated gastro-intestinal digestion. Food Chem 2014; 161: 142-7.
[http://dx.doi.org/10.1016/j.foodchem.2014.03.123] [PMID: 24837932]
[10]
Sousa AVB, Rodrigues-Junior FC, Santos GM, Moreira-Araújo RSR, Porto-Luz RGL. Determinação do teor de compostos fenólicos e atividade antioxidante da cajuína e do mel produzidos no Estado do Piauí - Brasil. Interfaces Científica. Saúde e Ambiente 2018; 6: 21-32.
[11]
Melo Cavalcante AA, Rübensam G, Picada JN, Gomes da Silva E, Fonseca Moreira JC, Henriques JAP. Mutagenicity, antioxidant potential, and antimutagenic activity against hydrogen peroxide of cashew (Anacardium occidentale) apple juice and cajuina. Environ Mol Mutagen 2003; 41(5): 360-9.
[http://dx.doi.org/10.1002/em.10158] [PMID: 12802807]
[12]
Melo-Cavalcante AA, Picada JN, Rubensam G, Henriques JAP. Antimutagenic activity of cashew apple (Anacardium occidentale Sapindales, Anacardiaceae) fresh juice and processed juice (cajuína) against methyl methanesulfonate, 4-nitroquinoline N-oxide and benzo[a]pyrene. Genet Mol Biol 2008; 31: 759-66.
[http://dx.doi.org/10.1590/S1415-47572008000400024]
[13]
Plaza M, Pozzo T, Liu J, Gulshan Ara KZ, Turner C, Nordberg Karlsson E. Substituent effects on in vitro antioxidizing properties, stability, and solubility in flavonoids. J Agric Food Chem 2014; 62(15): 3321-33.
[http://dx.doi.org/10.1021/jf405570u] [PMID: 24650232]
[14]
Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005; 53(6): 1841-56.
[http://dx.doi.org/10.1021/jf030723c] [PMID: 15769103]
[15]
Kim KH, Moon E, Choi SU, Kim SY, Lee KR. Polyphenols from the bark of Rhus verniciflua and their biological evaluation on antitumor and anti-inflammatory activities. Phytochemistry 2013; 92: 113-21.
[http://dx.doi.org/10.1016/j.phytochem.2013.05.005] [PMID: 23752101]
[16]
Minutolo M, Amalfitano C, Evidente A, Frusciante L, Errico A. Polyphenol distribution in plant organs of tomato introgression lines. Nat Prod Res 2013; 27(9): 787-95.
[http://dx.doi.org/10.1080/14786419.2012.704371] [PMID: 22788700]
[17]
AOAC Association of Official Analytical Chemists. Official methods of analysis. 20th ed. Arlington 2016.
[18]
Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965; 16: 144-58.
[19]
González-Aguilar GA, Villegas-Ochoa MA, Martínez-Téllez MA, Gardea AA, Ayala-Zavala JF. Improving antioxidant capacity of fresh-cut mangoes treated with UV-C. J Food Sci 2007; 72(3): S197-202.
[http://dx.doi.org/10.1111/j.1750-3841.2007.00295.x] [PMID: 17995814]
[20]
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999; 26(9-10): 1231-7.
[http://dx.doi.org/10.1016/S0891-5849(98)00315-3] [PMID: 10381194]
[21]
Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Food Sci Technol (Campinas) 1995; 28: 25-30.
[22]
Souza MM, Silva BD, Costa CSB, Badiale-Furlong E. Free phenolic compounds extraction from Brazilian halophytes, soybean and rice bran by ultrasound-assisted and orbital shaker methods. An Acad Bras Cienc 2018; 90(4): 3363-72.
[http://dx.doi.org/10.1590/0001-3765201820170745] [PMID: 30517215]
[23]
Siegel S. Nonparametric Statistics for the Behavioral Sciences. York, NY: New Mcgraw-Hill Book Company 1995.
[24]
Vidal RHL, Pereira CG. Ion exchange resin applied to obtain clarified cashew juice. Sep Sci Technol 2015; 50: 2737-46.
[http://dx.doi.org/10.1080/01496395.2015.1064961]
[25]
Damasceno LF, Fernandes FAN, Magalhaes MMA, Brito ES. Non-enzymatic browning in clarified cashew apple juice during thermal treatment: Kinetics and process control. Food Chem 2008; 106: 172-9.
[http://dx.doi.org/10.1016/j.foodchem.2007.05.063]
[26]
Danieli F, Costa LRLG, Silva LC, Hara ASS, Silva AA. Determination of vitamin C in sample orange juice in natura and commercials samples of orange juice pasteurized and bottled in Tetra Pak packages. J Health Sci Inst 2009; 27: 361-5.
[27]
Romagnolo DF, Selmin OI. Flavonoids and cancer prevention: a review of the evidence. J Nutr Gerontol Geriatr 2012; 31(3): 206-38.
[http://dx.doi.org/10.1080/21551197.2012.702534] [PMID: 22888839]
[28]
Lopes MMA, Miranda MRA, Moura CFH, Filho JE. Bioactive compounds and total antioxidant capacity of cashew apples (Anacardium occidentale L.) during the ripening of early dwarf cashew clones. Cienc Agrotec 2012; 36: 325-32.
[http://dx.doi.org/10.1590/S1413-70542012000300008]
[29]
Goli AH, Barzegar M, Sahari MA. Antioxidant activity and total phenolic compounds of pistachio (Pistachia vera) hull extracts. Food Chem 2005; 92: 521-5.
[http://dx.doi.org/10.1016/j.foodchem.2004.08.020]
[30]
Firdaus F, Zafeer MF, Anis E, Ahmad M, Afzal M. Ellagic acid attenuates arsenic induced neuro-inflammation and mitochondrial dysfunction associated apoptosis. Toxicol Rep 2018; 5: 411-7.
[http://dx.doi.org/10.1016/j.toxrep.2018.02.017] [PMID: 29854611]
[31]
Kumar D, Basu S, Parija L, et al. Curcumin and Ellagic acid synergistically induce ROS generation, DNA damage, p53 accumulation and apoptosis in HeLa cervical carcinoma cells. Biomed Pharmacother 2016; 81: 31-7.
[http://dx.doi.org/10.1016/j.biopha.2016.03.037] [PMID: 27261574]
[32]
Liang W-Z, Chou C-T, Cheng J-S, et al. The effect of the phenol compound ellagic acid on Ca(2+) homeostasis and cytotoxicity in liver cells. Eur J Pharmacol 2016; 780: 243-51.
[http://dx.doi.org/10.1016/j.ejphar.2016.03.057] [PMID: 27038520]
[33]
García-Niño WR, Zazueta C. Ellagic acid: Pharmacological activities and molecular mechanisms involved in liver protection. Pharmacol Res 2015; 97: 84-103.
[http://dx.doi.org/10.1016/j.phrs.2015.04.008] [PMID: 25941011]
[34]
de Oliveira LS, Thomé GR, Lopes TF, et al. Effects of gallic acid on delta - aminolevulinic dehydratase activity and in the biochemical, histological and oxidative stress parameters in the liver and kidney of diabetic rats. Biomed Pharmacother 2016; 84: 1291-9.
[http://dx.doi.org/10.1016/j.biopha.2016.10.021] [PMID: 27810786]
[35]
Verma S, Singh A, Mishra A. Gallic acid: molecular rival of cancer. Environ Toxicol Pharmacol 2013; 35(3): 473-85.
[http://dx.doi.org/10.1016/j.etap.2013.02.011] [PMID: 23501608]
[36]
Kannan RRR, Thangaradjou RAT, Anantharaman P. Phytochemical constituents, antioxidant properties and p-coumaric acid analysis in some seagrasses. Food Res Int 2013; 54: 1229-36.
[http://dx.doi.org/10.1016/j.foodres.2013.01.027]
[37]
Roy AJ, Stanely Mainzen Prince P. Preventive effects of p-coumaric acid on cardiac hypertrophy and alterations in electrocardiogram, lipids, and lipoproteins in experimentally induced myocardial infarcted rats. Food Chem Toxicol 2013; 60: 348-54.
[http://dx.doi.org/10.1016/j.fct.2013.04.052] [PMID: 23669407]
[38]
Sharma SH, Chellappan DR, Chinnaswamy P, Nagarajan S. Protective effect of p-coumaric acid against 1,2 dimethylhydrazine induced colonic preneoplastic lesions in experimental rats. Biomed Pharmacother 2017; 94: 577-88.
[http://dx.doi.org/10.1016/j.biopha.2017.07.146] [PMID: 28780474]
[39]
Queiroz C, Silva AJR, Lopes MLM, Fialho E, Valente-mesquita VL. Polyphenol oxidase activity, phenolic acid composition and browning in cashew apple (Anacardium occidentale L.) after processing. Food Chem 2011; 125: 128-32.
[http://dx.doi.org/10.1016/j.foodchem.2010.08.048]


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Article Details

VOLUME: 16
ISSUE: 4
Year: 2020
Published on: 12 July, 2020
Page: [585 - 591]
Pages: 7
DOI: 10.2174/1573401315666190206124545

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