Comparison Study on the Contents of Eight Flavonoids in three Different Processed Products of Scutellariae Radix using Ultra-high Performance Liquid Chromatography Coupled With Triple-Quadrupole Mass Spectrometry

Author(s): Yuedong Yang, Hao Zhi, Baofei Yan, Yi Tian, Jianping Shen, Xingyu Zhu*, Zhipeng Li*

Journal Name: Current Pharmaceutical Analysis

Volume 16 , Issue 6 , 2020

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Graphical Abstract:


Abstract:

Background: The simultaneous determination of multiple components in a sample is an important factor in the quality control of traditional Chinese medicines and can give an indication of potential clinical applications.

Introduction: A rapid and sensitive method has been introduced for the simultaneous quantitative analysis of eight bioactive flavonoid constituents from Scutellariae Radix using ultra-high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry.

Methods: The separation was performed on a Waters Acquity UPLC C18 column (2.1 mm×100 mm, 1.7 μm), under optimized mass spectrometry conditions, with a flow rate of 0.3 mL/min. The column temperature was maintained at 35°C and the injection volume was 3 μL.

Result: The method showed a good linear relationship of each component; all R2 values were above 0.9990 in the experiment. The RSDs of the precision test, repeatability test, stability test and recovery test were all not more than 2.86 %. We found that the total percentage amounts of the eight flavonoids were 22.19%, 18.63% and 10.86% in Raw Scutellariae Radix (RSR), Wine Scutellaria Radix (WSR) and Scutellaria Radix Charcoal (SRC) respectively.

Conclusion: The method was successfully applied to the simultaneous determination of the eight bioactive flavonoids of Raw Scutellariae Radix, Wine Scutellaria Radix and Scutellaria Radix Charcoal.

Keywords: Scutellariae radix, processing, UHPLC-TQ-MS/MS, quantification, flavonoids, quality control.

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Article Details

VOLUME: 16
ISSUE: 6
Year: 2020
Page: [690 - 697]
Pages: 8
DOI: 10.2174/1573412915666190206124150
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