Background: Persistent oxidative stress can lead to chronic inflammation and
mediate most chronic diseases including neurological disorders. Oleuropein has been
shown to be a potent antioxidant molecule in olive oil leaf having antioxidative properties.
Objective: The aim of this study was to investigate the protective effects of oleuropein
against oxidative stress in human glioblastoma cells.
Methods: Human glioblastoma cells (U87) were pretreated with oleuropein (OP) essential
oil 10 µM. After 30 minutes, 100 µM H2O2 was added to the cells for three hours. Cell
survival was quantified by colorimetric MTT assay. Glutathione level, total oxidant capacity,
total antioxidant capacity and nitric oxide levels were determined by using specific
spectrophotometric methods. The relative gene expression level of iNOS was performed by
Results: According to viability results, the effective concentration of H2O2 (100µM) significantly
decreased cell viability and oleuropein pretreatment significantly prevented the cell
losses. Oleuropein regenerated total antioxidant capacity and glutathione levels decreased
by H2O2 exposure. In addition, nitric oxide and total oxidant capacity levels were also decreased
after administration of oleuropein in treated cells.
Conclusion: Oleuropein was found to have potent antioxidative properties in human glioblastoma
cells. However, further studies and validations are needed in order to understand
the exact neuroprotective mechanism of oleuropein.