Effect of Wild Lactobacillus buchneri Strains on the Fermentation Profile and Microbial Populations of Sugarcane Silage

Author(s): Leandro D. da Silva*, Odilon G. Pereira, João P.S. Roseira, Mariele C.N. Agarussi, Vanessa P. da Silva, Thiago C. da Silva, Eliana dos S. Leandro, Rosinéa A. de Paula, Stefanie A. Santos, Karina G. Ribeiro, Sebastião de C.V. Filho

Journal Name: Recent Patents on Food, Nutrition & Agriculture

Volume 11 , Issue 1 , 2020

Become EABM
Become Reviewer
Call for Editor

Graphical Abstract:


Background: Sugarcane silage has been increasing as a feed in the tropics by dairy farmers. However, sugarcane normally had high yeast population that leads to intense alcoholic fermentation and excessive Dry-Matter (DM) loss during ensilage and after air exposure, as well. There are several patents that have recently shown the benefits of applying Lactobacillus buchneri in forage preservation.

Objective: This study aimed to investigate the changes in pH, DM, Water-Soluble Carbohydrates (WSC) and fermentation end product concentrations that occur in sugarcane silage with or without inoculation with L. buchneri after 45 days of ensiling.

Methods: Sugarcane plants were harvested with approximately 16 months of growth and chopped at 2 cm. Four strains of wild L. buchneri (56.1, 56.4, 56.9 and 56.26) and the commercial inoculant “Lalsil Cana” were evaluated. For all treatments, the theoretical application rate was 1.0 × 106 colony- forming units (cfu) per g of fresh weight. Data from the silo openings were analysed as a completely randomized design, with four replicates per treatment (inoculants).

Results: The treatment with L. buchneri affected the DM content, pH, Lactic Acid Bacteria (LAB) population, DM recovery, and concentrations of WSC, lactic acid, acetic acid and ethanol of sugarcane silage after 45 days of ensiling. Yeasts and molds populations and the concentrations of propionic and butyric acids were not affected by the treatments.

Conclusion: Lactobacillus buchneri 56.1 and 56.4 are considered the most suitable strains for improving the fermentation of sugarcane silage and thus are potential inoculants for silage production. At present, we are preparing the patent application.

Keywords: Dry matter recovery, lactic acid bacteria, organic acids, Saccharum officinarum L., water-soluble carbohydrates, yeast and mold.

Doonan BM, Kaiser AG, Stanley DF, Blackwood IF, Piltz JW, White AK. Silage in the farming system.Successful Silage DRDC:NSW Agriculture. 2004; 1-24.
Chiba S, Chiba H, Yagi M. A guide for silage making and utilization in the tropical regions. A publication of the Japanese Livestock Technology Association. In: 2005; p. 29.
Bernardes TF, do Rêgo AC. Study on the practices of silage production and utilization on Brazilian dairy farms. J Dairy Sci 2014; 97(3): 1852-61.
[http://dx.doi.org/10.3168/jds.2013-7181] [PMID: 24393176]
Pedroso AF, Rodrigues AA, Barioni Júnior W, Souza GB. Fermentation parameters, quality and losses in sugarcane silages treated with chemical additives and a bacterial inoculant. Rev Bras Zootec 2011; 40(11): 2318-22.
Driehuis F, Wilkinson JM, Jiang Y, Ogunade I, Adesogan AT. Silage review: Animal and human health risks from silage. J Dairy Sci 2018; 101(5): 4093-110.
[http://dx.doi.org/10.3168/jds.2017-13836] [PMID: 29685279]
Zielińska K, Fabiszewska A, Stefańska I. Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage. Chil J Agric Res 2015; 75(3): 298-306.
Rabelo CHS, Basso FC, Lara EC, Jorge LGO, Härter CJ, Mari LJ, et al. Effects of Lactobacillus buchneri as a silage inoculant or probiotic on in vitro organic matter digestibility, gas production and volatile fatty acids of low dry-matter whole-crop maize silage. Grass Forage Sci 2017; 72(3): 534-44.
Schmidt P, Nussio LG, Queiroz OCM, Santos MC, Zopollatto M, Filho SGT, et al. Effects of Lactobacillus buchneri on the nutritive value of sugarcane silage for finishing beef bulls. Rev Bras Zootec 2014; 43: 8-13.
Muck RE, Nadeau EMG, McAllister TA, Contreras-Govea FE, Santos MC, Kung L Jr. Silage review: Recent advances and future uses of silage additives. J Dairy Sci 2018; 101(5): 3980-4000.
[http://dx.doi.org/10.3168/jds.2017-13839] [PMID: 29685273]
Borreani G, Tabacco E, Schmidt RJ, Holmes BJ, Muck RE. Silage review: Factors affecting dry matter and quality losses in silages. J Dairy Sci 2018; 101(5): 3952-79.
[http://dx.doi.org/10.3168/jds.2017-13837] [PMID: 29685272]
Kleinschmit DH, Kung L Jr. A meta-analysis of the effects of Lactobacillus buchneri on the fermentation and aerobic stability of corn and grass and small-grain silages. J Dairy Sci 2006; 89(10): 4005-13.
[http://dx.doi.org/10.3168/jds.S0022-0302(06)72444-4] [PMID: 16960077]
Silva LD, Pereira OG, Silva TC, Leandro ES, Paula RA, Santos SA, et al. Effects of Lactobacillus buchneri isolated from tropical maize silage on fermentation and aerobic stability of maize and sugarcane silages. Grass Forage Sci 2018; 73: 660-70.
AOAC. In: Official methods of analysis. 15th ed. Arlington, VA, USA: Association of Official Analytical Chemistry 1990.
Nelson N. A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 1944; 153: 375-80.
Siegfried VR, Ruckemann H, Stumpf G. Method for the determination of organic acids in silage by high performance liquid chromatography. Landwirt Forsch 1984; 37: 298-304.
Jobim CC, Nussio LG, Reis RA, Schmidt P. Methodological advances in evaluation of preserved forage quality. Rev Bras Zootec 2007; 36: 101-19.
Weißbach F, Strubelt C. Correcting the dry matter content of maize silages as a substrate for biogas production. Landtechnik 2008; 63: 82-3.
Freitas AWP, Pereira JC, Rocha FC, Costa MG, Fernando de Paula Leonel FP, Ribeiro MD. Avaliação da qualidade nutricional da silagem de cana-de-açúcar com aditivos microbianos e enriquecida com resíduo da colheita de soja. Rev Bras Zootec 2006; 35(1): 38-47.
Ávila CS, Pinto JC, Sugawara MS, Silva MS, Schwan RF. Qualidade da silagem de cana-de açúcar inoculada com uma cepa de Lactobacillus buchneri. Acta Sci Anim Sci 2008; 30: 255-61.
McDonald P, Henderson AR, Heron SJE. The biochemistry of silage. Marlow, UK: Chalcombe Publications 1991; p. 340.
Ávila CLS, Carvalho BF, Pinto JC, Duarte WF, Schwan RF. The use of Lactobacillus species as starter cultures for enhancing the quality of sugar cane silage. J Dairy Sci 2014; 97(2): 940-51.
[http://dx.doi.org/10.3168/jds.2013-6987] [PMID: 24359831]
Carvalho BF, Ávila CLS, Miguel MGCP, Pinto JC, Santos MC, Schwan RF. Aerobic stability of sugar-cane silage inoculated with tropical strains of lactic acid bacteria. Grass Forage Sci 2014; 70: 308-23.
Priest FG, Goodfellow M, Eds. Applied Microbial Systematics. Amsterdam, Boston: Kluwer Academic Publishers 2000.
Danner H, Holzer M, Mayrhuber E, Braun R. Acetic acid increases stability of silage under aerobic conditions. Appl Environ Microbiol 2003; 69(1): 562-7.
[http://dx.doi.org/10.1128/AEM.69.1.562-567.2003] [PMID: 12514042]
Liu S, Skinner-Nemec KA, Leathers TD. Lactobacillus buchneri strain NRRL B-30929 converts a concentrated mixture of xylose and glucose into ethanol and other products. J Ind Microbiol Biotechnol 2008; 35(2): 75-81.
[http://dx.doi.org/10.1007/s10295-007-0267-8] [PMID: 17940817]
Ogunade IM, Jiang Y, Pech Cervantes AA. Kim DH, Oliveira AS,Vyas D, et al. Bacterial diversity and composition of alfalfa silage as analyzed by Illumina MiSeq sequencing: Effects of Escherichia coli O157:H7 and silage additives. J Dairy Sci 2018; 101(3): 2048-59.
[http://dx.doi.org/10.3168/jds.2017-12876] [PMID: 29274960]
Pedroso AF, Nussio LG, Loures DRS, Paziani SF, Ribeiro JL, Mari LJ, et al. Fermentation, losses, and aerobic stability of sugarcane silages treated with chemical and bacterial additives. Sci Agric 2008; 65: 567-691.
Ávila CLS, Pinto JC, Figueiredo HCP, Schwan RF. Effects of an indigenous and a commercial Lactobacillus buchneri strain on quality of sugar cane silage. Grass Forage Sci 2009; 64: 384-94.
Taylor CC, Kung L Jr. The effect of Lactobacillus buchneri 40788 on the fermentation and aerobic stability of high moisture corn in laboratory silos. J Dairy Sci 2002; 85(6): 1526-32.
[http://dx.doi.org/10.3168/jds.S0022-0302(02)74222-7] [PMID: 12146485]
Driehuis F, Elferink SJ, Spoelstra SF. Anaerobic lactic acid degradation during ensilage of whole crop maize inoculated with lactobacillus buchneri inhibits yeast growth and improves aerobic stability. J Appl Microbiol 1999; 87(4): 583-94.
[http://dx.doi.org/10.1046/j.1365-2672.1999.00856.x] [PMID: 10583687]

open access plus

Rights & PermissionsPrintExport Cite as

Article Details

Year: 2020
Published on: 28 April, 2020
Page: [63 - 68]
Pages: 6
DOI: 10.2174/2212798410666190128101343

Article Metrics

PDF: 42