Background: Shilajit is a natural herbomineral ethnomedicinal substance used in Indian
traditional systems of medicine since centuries as a rejuvenator, anti-aging and for several health
conditions/ailments. Shilajit composition has exhibited considerable variability from different geographical
sites and hence there is a need to determine its composition and concentration of bioactive
compounds to correlate it with pharmacological activities.
Objective: To determine the chemical and mineral constitution of Shilajit obtained from Himachal
Method: In the present study, Shilajit was chemically characterized by Fourier Transform Infrared
(FTIR) spectroscopy, Gas Chromatography-Mass Spectrometry (GC-MS), Inductively-Coupled
Plasma Mass Spectrometry (ICP-MS), UV-Vis spectroscopy and High-Performance Liquid Chromatography
Results: Chemical elucidation of crude Shilajit from three geographical sites revealed the presence
of humic acid, fulvic acid, dibenzo-α-pyrones and several other organic constituents in crude Shilajit.
The presence of metals/trace elements was evident in Shilajit samples with potassium being predominant
followed by magnesium, calcium, sodium, iron and aluminium.
Conclusion: Characterization of Shilajit from Himachal Pradesh supported the structural details of
Shilajit already known and also revealed variations in key chemical parameters which might be due
to geographical variations and ecological conditions which determine its natural synthesis.