The Alkaloids and Bioactivities of Ethanol Extract from a Traditional Remedy of Vietnam

Author(s): Nguyen H. Sa, Trinh T. Thuy*, Tran D. Quan, Dao D. Thien, Nguyen T. Tam, Le C. Hoan, Le T.H. Nhung, Huynh T.N. Ni, Sabrina Adorisio, Domenico V. Delfino.

Journal Name: The Natural Products Journal

Volume 10 , Issue 1 , 2020

Become EABM
Become Reviewer

Graphical Abstract:


Abstract:

Background and Objective: In Vietnam, “Dai trang hoan ba Giang” (BG), which belongs to herbalist “Ba Giang”, has been one of the famous herbal remedies for ulcerative colitis and diarrhea for over 100 years. However, up to the present, the main BG’s chemical constituents have not been investigated. Therefore, this study aims to investigate the phytochemistry and antiproliferative activity of isolated compounds from BG on Acute Myeloid Leukemia (OCI-AML) cells as well as evaluating its safety by the acute and subchronic toxicological tests.

Methods: Compounds from herbmaterial were isolated by using column chromatography. Their structures were determined by combining spectral analysis and comparison with reported data. The concentrations of components were were determined by HPLC/DAD analysis. Anti-proliferative activity in OCI-AML cell line of isolated compounds was carried out by flow cytometry analysis. The acute and subchronic oral toxicity in micewere appraised by observingdaily for clinical manifestations of toxicity while the experiment lasted.

Results: In this study, driedpowders of materials were extracted by 70% EtOH to afford an extract (DTBG). Two compounds, including palmatine (1) and berberine (2) were isolated from DTBG. The DTBG inhibited the growth of OCI-AML by a significant increase of caspase-8-independent apoptosis as measured by propidium iodide flow cytometry analysis and caspase-3 activation. In the acute study, DTBG is well tolerated, non-toxic and non-lethal to mice under the present experimental conditions. In thesubchronic study in mice, DTBG did not impact on weight gain, blood, liver and kidney function.

Conclusion: Overall, the main chemical constituents of DTBG and its effect on proliferation of acute myeloid leukemia cells were reported for the first time. Moreover, the results suggest that, DTBG did not express any significant toxic effect in mice. Hence, the extract can be utilized for pharmaceutical formulations.

Keywords: Alkaloids, OCI cells, apoptosis, acute toxicity, subchronic toxicity, leukemia, spectral analysis.

[1]
Xavier, R.J.; Podolsky, D.K. Unravelling the pathogenesis of inflammatory bowel disease. Nature, 2007, 448(7152), 427-434.
[2]
Ma, B.L.; Ma, Y.M.; Shi, R.; Wang, T.M.; Zhang, N.; Wang, C.H.; Yang, Y. Identification of the toxic constituents in Rhizoma Coptidis. J. Ethnopharmacol., 2010, 128(2), 357-364.
[3]
Iizuka, N.; Miyamoto, K.; Okita, K.; Tangoku, A.; Hayashi, H.; Yosino, S.; Abe, T.; Morioka, T.; Hazama, S.; Oka, M. Inhibitory effect of Coptidis rhizoma and berberine on the proliferation of human esophageal cancer cell lines. Cancer Lett., 2000, 148(1), 19-25.
[4]
Zhou, X.; Lin, X.; Xiong, Y.; Jiang, L.; Li, W.; Li, J.; Wu, L. Chondroprotective effects of palmatine on osteo arthritis in vivo and in vitro: A possible mechanism of inhibiting the Wnt/β-catenin and Hedgehog signaling pathways. Int. Immunopharmacol., 2016, 34, 129-138.
[5]
Yao, C.M.; Yang, X.W. Bioactivity-guided isolation of polyacetylenes with inhibitory activity against NO production in LPS-activated RAW264.7 macrophages from the rhizomes of Atractylodes macrocephala. J. Ethnopharmacol., 2014, 151(1), 791-799.
[6]
Zhang, N.; Liu, C.; Sun, T.M.; Ran, X.K.; Kang, T.G.; Dou, D.Q. Two new compounds from Atractylodes macrocephala with neuroprotective activity. J. Asian Nat. Prod. Res., 2017, 19(1), 35-41.
[7]
Hoang, L.S.; Tran, M.H.; Lee, J.S.; Ngo, T.Q.M.; Woo, M.H.; Min, B.S. Inflammatory inhibitory activity of sesquiterpenoids from Atractylodes macrocephala rhizomes. Pharm. Soc. Jpn., 2016, 64(5), 507-511.
[8]
Huang, H.L.; Lin, T.W.; Huang, Y.L.; Huang, R.L. Induction of apoptosis and differentiation by atractylenolide-1 isolated from Atractylodes macrocephala in human leukemia cells. Bioorg. Med. Chem. Lett., 2016, 26(8), 1905-1909.
[9]
Jung, H.A.; Yoon, N.Y.; Bae, H.J.; Min, B.S.; Choi, J.S. Inhibitory activities of the alkaloids from Coptidis rhizoma against aldose reductase. Arch. Pharm. Res., 2008, 31(11), 1405-1412.
[10]
Pozzesi, N.; Pierangeli, S.; Vacca, C.; Falchi, L.; Pettorossi, V.; Martelli, M.P.; Thuy, T.T.; Ninh, P.T.; Liberati, A.M.; Riccardi, C.; Sung, T.V. Maesopsin 4-O-beta-D-glucoside, a natural compound isolated from the leaves of Artocarpus tonkinensis, inhibits proliferation and up-regulates HMOX1, SRXN1 and BCAS3 in acute myeloid leukemia. J. Chemother., 2011, 23(3), 150-157.
[11]
Yeo, D.; Bouagnon, R.; Djyh, B.N.; Tuo, C.; N’guessan, J.D. Acute and subacute toxic study of aqueous leaf extract of Combretum molle. Trop. J. Pharm. Res., 2012, 11(2), 217-223.
[12]
Gandhare, B.; Kavimani, S.; Rajkapoor, B. Acute and subacute toxicity study of methanolic extract of Ceiba pentandra (Linn.) gaertn. on rats. J. Sci. Res., 2013, 5(2), 315-324.
[13]
Wang, D.; Liu, Z.; Guo, M.; Liu, S. Structural elucidation and identification of alkaloids in Rhizoma coptidis by electrospray ionization tandem mass spectrometry. J. Mass Spectrom., 2004, 39, 1356-1365.


Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 10
ISSUE: 1
Year: 2020
Page: [20 - 25]
Pages: 6
DOI: 10.2174/2210315509666190103102949
Price: $25

Article Metrics

PDF: 15
HTML: 2