Optimization Growth of Spirulina (Arthrospira) Platensis in Photobioreactor Under Varied Nitrogen Concentration for Maximized Biomass, Carotenoids and Lipid Contents

Author(s): Hanaa H. Abd El Baky*, Gamal S. El Baroty, Enas M. Mostafa

Journal Name: Recent Patents on Food, Nutrition & Agriculture

Volume 11 , Issue 1 , 2020

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Graphical Abstract:


Aims and Background: Spirulina (Arthrospira) platensis (SP) microalgae were cultured in Zarrouk Medium (ZM), containing three nitrogen concentrations (N-limited, N-optimal and Nrich medium) in ten liter-photo-bioreactor (10 L PBR) for 15-days, in order to study changes in lipid compounds (total carotenoids and total lipids and their effect on fatty acid profile). Based on US patent, the yield of bioactive compounds (such as gamma-linolenic acid GLA, C18:3) extracted from microalgae biomass, mainly depends on the extraction processes (1). GLA has much attention with respect to its therapeutic properties such as its ability to decrease blood cholesterol levels.

Methods: The impact of the addition of N in cultures of S. platensis in terms of growth, biomasses and induced lipid compounds (total carotenoids and total lipid contents and its fatty acid profile), as well as the Sonication (SON) and Microwave (MIC) process as aiding techniques for lipid extraction compared with a Cold Condition (COL), was examined. GC/MS method was used to determine the fatty acid profile of lipid extract of SP cultures.

Results: In all S. platensis tested culture, the SP was growing successfully, with varying degrees. In N-rich media, the highest cell growth rate and biomass yield were obtained compared with that recorded in other cultures. Under an N-limited condition, SP had higher Total Carotenoids (TCAR, 45.54 mg/g dw) and total lipid contents (TL, 29.51%± 1.92 g/100g dw) compared with that recorded either in N-rich (11.2 mg/g dw) or in N-optimal (6.23 mg/g dw) cultures. Thus, SP copes with the N -stress by altering the metabolic pathways towards inducing lipid biosynthesis. To maximize the TL and TCAR extraction yields, from N-limited cultures, a set of operating process was applied including the Sonication (SON) and Microwave (MIC), which were used as aiding techniques for lipid extraction compared with the Cold Condition (COL) techniques. The results showed that the extraction efficiency of the S. platensis TL increased in the following order: MIC (29.51%± 1.92) > SON (25.46% ± 1.65> COL (20.43% ±1.43). In a comparative study for its fatty acid profiles (FAPs) among all SP cultures, lipids were analyzed by GC/MS. The predominant fatty acids (>10%, of total FA) were found to be myristic acid (C14:0, MA), palmitic acid (C16:0, PA) and oleic acid (C18:1).

Conclusion: The study concluded that the N-limited condition was found to have a strong influence on biomass dry weight and lipid contents and total carotenoids in SP cells compared to either Nrich or N-optimal conditions. The use of sonication and the microwave techniques lead to a great increase in the extraction of lipid contents and in high amount Polyunsaturated Fatty Acids (PUFAs) in N-limited cultures, in particular, the omega-6 (ω 6) and omega-3 (ω 3) of the essential C18 fatty acids. It seems that the SP rich in lipid content with a high amount of GLC produced under nitrogen limitation in PBR conditions can be used as a food additive or as a nutritional supplement.

Keywords: Microalgae, Spirulina platensis, photobioreactor, lipids and carotenoids, biomass, N-limited cultures.

Zhu Y, Li Y, Wang K, Bai K, Kuang T. Simplified method for extracting a great lot phycocyanin. CN Patent 1687440 (2005).
Moreira LM, Ribeiro AC, Duarte FA, Morais MG, Soares LAS. S. platensis biomass cultivated in Southern Brazil as a source of essential minerals and other nutrients. Afr J Food Sci 2013; 7(12): 451-5.
Abd El Baky HH, El Baz FK, El-Baroty GS. Enhancement of antioxidant production in Spirulina plantensis under oxidative stress. Acta Physiol Plant 2009; 31(3): 62331.
Abd El Baky HH, El Baz FK, El-Baroty GS. Production of phenolic compounds from Spirulina maxima microalgae and its protective effects in vitro toward hepatotoxicity model. Afr J Pharm Pharmacol 2009; 3(4): 133-9.
Yaakob Z, Ali E, Zainal A, Mohamad M, Takriff MS. An overview: biomolecules from microalgae for animal feed and aquaculture. J Biol Res (Thessalon) 2014; 21(1): 6.
[http://dx.doi.org/10.1186/2241-5793-21-6] [PMID: 25984489]
Vaz BDS, Moreira JB, Morais MGD, Costa JAV. Microalgae as a new source of bioactive compounds in food supplements. Curr Opin Food Sci 2009; 7(1): 73-7.
Abd El Baky HH, El Baroty GS, Ibrahem EA. Ibrahem. Functional characters evaluation of biscuits sublimated with pure phycocyanin isolated from Spirulina and Spirulina biomass. Nutr Hosp 2015; 32(1): 231-41.
[PMID: 26262722]
Greque de Morais M, Greque de Morais E, Vaz BdaS, Gonçalves CF, Lisboa C, Costa JAV. Nanoencapsulation of the bioactive compounds of Spirulina with a microalgal biopolymer coating. J Nanosci Nanotechnol 2016; 16(1): 81-91.
[http://dx.doi.org/10.1166/jnn.2016.10899] [PMID: 27398435]
Borowitzka MA. High-value products from microalgae: their development and commercialization. J Appl Phycol 2013; 25(3): 743-56.
Colla LM, Furlong EB, Costa JAV. Anti-oxidant Propretés of Spirulina (Arthospira) platensis cultivated under different temperatures and nitrogen regimes. Braz Arch Biol Technol 2007; 50(1): 161-7.
Hetta M, Mahmoud R, El-Senousy W, Ibrahim M, El-Taweel G, Ali G. Antiviral and antimicrobial activities of S. platensis. World J Pharm Pharm Sci 2014; 3(6): 31-9.
Fukami K, Nishijima T, Hata Y. Availability of deep seawater and effects of bacteria isolated from deep seawater on the mass culture of food microalga Chaetoceros ceratosporum. Nippon Suisan Gakkaishi 1992; 58(5): 931-6.
Thomson KJ. The State of Food and Agriculture - Biofuels - Prospects, Risks and Opportunities. Rome, Italy FAO 2008; 147(4): 503.
Febles CI, Arias A, Gill-Rodriguez MC. In vitro study of antimicrobial activity in algae (Chlorophyta, Phaeophyta and Rhodophyta) collected from coast of Tenerife. Anu Estud Canarios 1995; 34: 181-92. [in Spanish].
Ibañez E, Cifuentes A. Benefits of using algae as natural sources of functional ingredients. J Sci Food Agric 2013; 93(4): 703-9.
[http://dx.doi.org/10.1002/jsfa.6023] [PMID: 23339029]
Abd El-Baky HH, El-Baroty GS. Characterization and bioactivity of phycocyanin isolated from Spirulina maxima grown under salt stress. Food Funct 2012; 3(4): 381-8.
[http://dx.doi.org/10.1039/c2fo10194g] [PMID: 22234291]
Abd El Baky HH, El-Baroty GS, Bouaid A. Lipid induction in Dunaliella salina culture aerated with various levels CO2 and its biodiesel characterizations. J Aquac Res Dev 2014; 5(3): 1-6.
Sheehan J, Dunahay T, Benemann J, Roessler PA. Look Back at the U.S. Department of Energy's Aquatic Species Program: Biodiesel from Algae; Close-Out Report. US 1998.
El Baroty GS, El Baz FK, Abd-Elmoein I. Abd El-Baky H H, Ail MM, Ibrahim E A. Evaluation of glycolipids of some Egyptian marine algae as a source of bioactive substances. Electron J Envi Agric Food Chem 2011; 10(4): 2114-218.
Klok AJ, Lamers PP, Martens DE, Draaisma RB, Wijffels RH. Edible oils from microalgae: insights in TAG accumulation. Trends Biotechnol 2014; 32(10): 521-8.
[http://dx.doi.org/10.1016/j.tibtech.2014.07.004] [PMID: 25168414]
Yen HW, Hu IC, Chen CY, Ho SH, Lee DJ, Chang JS. Microalgaebased biorefinery--from biofuels to natural products. Bioresour Technol 2013; 135(166-74): 166-74.
[http://dx.doi.org/10.1016/j.biortech.2012.10.099] [PMID: 23206809]
Zarrouk C. Contribution à l'étude d'une cyanophycée: Influence de divers facteurs physiques et chimiques sur la croissance et la photosynthèse de Spirulina maxima (Setch et Gardner) Geitler. Ph.D Thesis, Université De Paris, Paris 1966.
Abd El Baky HH, El-Baroty GS, Bouaid A, Martinez M, Aracil J. Enhancement of lipid accumulation in Scenedesmus obliquus by Optimizing CO2 and Fe3+ levels for biodiesel production. Bioresour Technol 2012; 119: 429-32.
[http://dx.doi.org/10.1016/j.biortech.2012.05.104] [PMID: 22727605]
AOAC In: Official Methods of AnalysisHowitz W, Ed. 1980; pp. 734-40.
Abd El Baky HH, El Baroty GS. Optimization of growth conditions for purification and production of L-asparaginase by Spirulina maxima. Evid Based Complement Alternat Med 2016; 2016Article ID 1785938
[http://dx.doi.org/10.1155/2016/1785938] [PMID: 27525017]
Alupului A, Calinescu I, Lavric V. Microwave extraction of active principles from medicinal plants. Sci Bull B Chem Mater Sci UPB 2012; 74(2): 129-42.
Zhang Q, Hong Y. Comparison of growth and lipid accumulation properties of two oleaginous microalgae under different nutrient conditions. Front Environ Sci Eng 2014; 8(5): 703-9.
Abd El Baky HH, El-Baroty GS. The potential use of microalgal carotenoids as dietary supplements and natural preservative ingredient. J Aquat Food Prod Technol 2013; 22(4): 392-406.
Abd El Baky HH, El-Baroty GS, Ibrahem EA. Antiproliferation and antioxidant properties of lipid extracts of the microalgae Scenedesmus obliquus grown under stress conditions. Pharma Chem 2014; 6(5): 24-34.
Abd El Baky HH, El Baz FK, El-Baroty GS. Natural preservative ingredient from marine alga Ulva lactuca L. Int J Food Sci Technol 2009; 44(9): 1688-95.
Ho SH, Chen CY, Chang JS. Effect of light intensity and nitrogen starvation on CO2 fixation and lipid/carbohydrate production of an indigenous microalga Scenedesmus obliquus CNW-N. Bioresour Technol 2012; 113: 244-52.
[http://dx.doi.org/10.1016/j.biortech.2011.11.133] [PMID: 22209130]
Benavente-Valdés JR, Aguilar C, Contreras-Esquivel JC, Méndez-Zavala A, Montañez J. Strategies to enhance the production of photosynthetic pigments and lipids in chlorophycae species. Biotechnol Rep (Amst) 2016; 10: 117-25.
[http://dx.doi.org/10.1016/j.btre.2016.04.001] [PMID: 28352532]
El Baz FK, El-Baroty GS, Abd El Baky HH, Abd El-Salam OI, Ibrahim EA. Structural characterization and biological activity of sulfolipids from selected marine Algae. Grasas Aceites 2013; 64(5): 561-71.
Abedini Najafabadi H, Malekzadeh M, Jalilian F, Vossoughi M, Pazuki G. Effect of various carbon sources on biomass and lipid production of Chlorella vulgaris during nutrient sufficient and nitrogen starvation conditions. Bioresour Technol 2015; 180: 311-7.
[http://dx.doi.org/10.1016/j.biortech.2014.12.076] [PMID: 25621723]
Bensehaila S, Doumandji A, Boutekrabt L, Manafikkhi H, Peluso I, Bensehaila K, et al. The nutritional quality of Spirulina platensis of Tamenrasset, Algeria. Afr J Biotechnol 2015; 14(19): 1649-54.
Muhling M, Belay A, Whitton BA. Variation in fatty acid composition of Arthrospira (Spirulina) strains. J Appl Phycol 2005; 17(2): 137-46.
Bhakar RN, Kumar R, Pabbi S. Total lipids and fatty acid profile of different Spirulina strains as affected by salinity and incubation time. Vegetos 2013; 26: 148-54.

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Article Details

Year: 2020
Page: [40 - 48]
Pages: 9
DOI: 10.2174/2212798410666181227125229

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