Background: Disaccharide core 1 (Galβ1-3GalNAc) is a common O-glycan structure in
nature. Biochemical studies have confirmed that the formation of the core 1 structure is an important
initial step in O-glycan biosynthesis and it is of great importance for human body.
Objective: Our study will provide meaningful and useful sights for O-glycan synthesis and their bioassay.
And all the synthetic glycosides would be used as intermediate building blocks in the scheme
developed for oligosaccharide construction.
Methods: In this article, we firstly used chemical procedures to prepare core 1 and its derivative,
and a novel disaccharide was efficiently synthesized. The structures of the synthesized compounds
were elucidated and confirmed by 1H NMR, 13C NMR and MS. Then we employed three human gut
symbionts belonging to Bacteroidetes, a predominantphyla in the distal gut, as models to study the
bioactivity of core 1 and its derivative on human gut microbiota.
Results: According to our results, both core 1 and derivative could support the growth of B. fragilis,
especially the core 1 derivative, while failed to support the growth of B. thetaiotaomicron and B.
Conclusion: This suggested that the B. fragilis might have the specificity glycohydrolase to cut the
glycosidic bond for acquiring monosaccharide.