Investigation of the Biological Activities of Different Extracts from Dipsacus laciniatus Aerial Parts

Author(s): Turgut Taşkın*, Yeliz Akkılıç, Berna Özbek Çelik, İsmail Şenkardeş, Özlem B. Özakpınar.

Journal Name: The Natural Products Journal

Volume 10 , Issue 1 , 2020

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Graphical Abstract:


Background: The aerial parts of Dipsacus laciniatus are traditionally used in the treatment of colds as cold tea; decoction prepared from its roots and aerial parts are used for the treatment of diabetes and cancer, respectively. The extraction procedures and solvents are important steps in the processing of bioactive constituents from the plant materials.

Objective: The aim of this study was to evaluate in vitro antioxidant, antimicrobial, anticholinesterase and cytotoxic activities for the first time in plant’s different extracts.

Methods: The antioxidant activities of extracts were examined using FRAP and CUPRAC methods, including total phenolic and flavonoids contents. In addition, the antimicrobial, anticholinesterase and cytotoxic activities of extracts were investigated using microdilution, Ellman and MTT methods, respectively.

Results: In the present study, methanol extract showed stronger iron (III) ion reduction (FRAP) and copper (II) ion reducing antioxidant capacity (CUPRAC) than other extracts and also contained higher total phenolic and flavonoid compounds. It was also found that there was a linear relationship between antioxidant activity and phenolic compounds in this study. It was demonstrated that the chloroform and methanol extracts showed moderate antimicrobial activity against S. epidermidis and C. albicans strains. The chloroform extract had a strong and selective cytotoxic activity for the MCF-7 and HT-29 cell lines, while the methanol extract showed low cytotoxic activity on the same cell lines. In addition, according to the obtained data, chloroform extract showed a toxic effect on normal cell line (NIH/3T3), but other extracts showed no toxic effect.

Conclusion: Since methanol extract has no toxic effect on normal cell line and has strong antioxidant, anticholinesterase and antimicrobial activity, it is thought that this extract may be useful as an antioxidant, anticholinesterase and antimicrobial agents in the future.

Keywords: Anticholinesterase, antimicrobial, antioxidant, cytotoxic, Dipsacus laciniatus, FRAP, CUPRAC.

Veeresham, C. Natural products derived from plants as a source of drugs. J. Adv. Pharm. Technol. Res., 2012, 3, 200-201.
Davis, P.H. Flora of Turkey and the East Aegean Islands; Edinburgh, Edinburgh University Press,. , 1972, pp. 250-260.
Papp, N.; Bartha, S.; Boris, G.; Balogh, L. Traditional uses of medicinal plants for respiratory diseases in Transylvania. Nat. Prod. Commun., 2011, 6, 1459-1460.
Arıtuluk, Z.C.; Ezer, N. Halk arasında diyabete karşı kullanılan bitkiler (Türkiye)-II. Hacettepe Üniv. Ecz. Fak. Dergisi, 2012, 32, 179-208.
Tuzlacı, E.; Doğan, A. Turkish folk medicinal plants, IX: Ovacık (Tunceli). Marmara Pharm. J., 2010, 14, 136-143.
Tuzlacı, E. Türkiye Bitkileri Geleneksel İlaç Rehberi; İstanbul Tıp Kitabevleri, 2016, pp. 1212-1213.
Murugan, R.; Parimelazhagan, T. Comparative evaluation of different extraction methods for antioxidant and anti-inflammatory properties from Osbeckia parvifolia Arn. An in vitro approach. J King Saud Univ, 2014, 26, 267-275.
Samatha, T.; Shyamsundarachary, R.; Srınıvas, P.; Swamy, N.R. Quantification of total phenolic and total flavonoid contents in extracts of Oroxylum indicum L. Kurz. Asian J. Pharm. and Clin. Res., 2012, 5, 177-179.
Ozsoy, N.; Can, A.; Yanardağ, R.; Akev, N. Antioxidant activity of Smilax excelsa L. leaf extracts. Food Chem., 2008, 110, 571-583.
Benzie, I.F.F.; Strain, J.J. The Ferric Reducing Ability of Plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal. Biochem., 1996, 239, 70-76.
Apak, R.; Güçlü, K.; Özyürek, M.; Karademir, S.E. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J. Agric. Food Chem., 2004, 52, 7970-7981.
Ellman, G.L.; Courtney, K.D.; Andress, V.; Featherstone, R.M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol., 1961, 7, 88-95.
Clinical and Laboratory Standards Institute, Performance Standards for Antimicrobial Susceptibility Testing., Wayne, Pennsylvania. 2006.
Clinical and Laboratory Standards Institute, Performance Standards for Antimicrobial Susceptibility Testing., Wayne, Pennsylvania. 2000.
Perez, C.; Pauli, M.; Bazerque, P. An antibiotic assay by the agar-well diffusion method. Acta Bio. Et. Med. Experi, 1990, 15, 113-115.
Sgouras, D.; Duncan, R. Methods for the evaluation of biocompatibility of soluble syntehic polymer which have potential for biomedical use: 1-Use of the tetrazolium-based colorimetric assay (MTT) as a preliminary screen for evaluation of in vitro cytotoxicity. J. Mater. Sci. Mater. Med., 1990, 1, 61-68.

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Article Details

Year: 2020
Page: [15 - 19]
Pages: 5
DOI: 10.2174/2210315509666181214153533
Price: $25

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