Correlation Between Insulin Levels and Thyroid Hormones in Diabetic Animals After Caffeine Consumption Associated with Exercise

Author(s): Luiz Augusto da Silva*, Jéssica Wouk, Vinícius Müller Reis Weber, Pablo de Almeida, Julio C.L. Martins, Carlos R.M. Malfatti, Raul Osiecki

Journal Name: Current Nutrition & Food Science

Volume 16 , Issue 3 , 2020


Become EABM
Become Reviewer
Call for Editor

Graphical Abstract:


Abstract:

Introduction: Thyroid hormones (TH) are important determinants of glucose homeostasis, and in contrast, insulin is the first hormone responsible for glycemic control.

Objective: The objective of the present study was to correlate the levels of insulin and thyroid hormones in diabetic animals after caffeine consumption associated with physical exercise.

Methods: A total of 48 animals, 60 days old were allocated in eight experimental groups: Control, Diabetic, Exercise, Diabetes + exercise, Caffeine, Diabetes + Caffeine, Caffeine + Exercise, and Diabetes + Exercise + Caffeine. Diabetes model was induced by intraperitoneal administration of 120 mg/kg of alloxan. On the test day, 6 mg/kg of caffeine was administrated 30 minutes before physical exercise. After, animals performed a 60 minutes’ session of predominantly aerobic exercise, using an overload of 6% of their body’s weight. Blood has been collected by a caudal puncture to future insulin, TSH, T3, and T4 analyses.

Results: After caffeine treatment and training, insulin values were higher for the control groups (231%) when compared to the diabetic groups. A significant increase in plasmatic insulin concentration was found in caffeine group (95%) and Exercise+Caffeine group (56%) when compared to Control and Exercise groups. TSH values were increased for Diabetes, Diabetes+Caffeine and Diabetes+ Exercise+Caffeine groups (30%) compared to the other groups. A reduction in T4 values occurred in the animals of groups Diabetes+Exercise and Diabetes +Caffeine (66%) compared to the Control group. T3 values were significantly increased for the Diabetes+Exercise group (70%) when compared to the Diabetes+Exercise+Caffeine group.

Conclusion: Physical exercise and caffeine consumption were able to promote hormonal changes in diabetic animals after 30 days of training. The study showed a reduction in the serum concentration of thyroid hormones, but insulin levels were higher.

Keywords: Diabetes mellitus, disease, glucose, metabolism, physical effort, rats.

[1]
Kim SR, Tull ES, Talbott EO, Vogt MT, Kuller LH. A hypothesis of synergism: the interrelationship of T3 and insulin to disturbances in metabolic homeostasis. Med Hypotheses 2002; 59(6): 660-6.
[http://dx.doi.org/10.1016/S0306-9877(02)00211-6] [PMID: 12445506]
[2]
Wennlund A, Felig P, Hagenfeldt L, Wahren J. Hepatic glucose production and splanchnic glucose exchange in hyperthyroidism. J Clin Endocrinol Metab 1986; 62(1): 174-80.
[http://dx.doi.org/10.1210/jcem-62-1-174] [PMID: 3510000]
[3]
Lambadiari V, Mitrou P, Maratou E, et al. Thyroid hormones are positively associated with insulin resistance early in the development of type 2 diabetes. Endocrine 2011; 39(1): 28-32.
[http://dx.doi.org/10.1007/s12020-010-9408-3] [PMID: 21072691]
[4]
Ortega E, Koska J, Pannacciulli N, Bunt JC, Krakoff J. Free triiodothyronine plasma concentrations are positively associated with insulin secretion in euthyroid individuals. Eur J Endocrinol 2008; 158(2): 217-21.
[http://dx.doi.org/10.1530/EJE-07-0592] [PMID: 18230829]
[5]
Roos A, Bakker SJ, Links TP, Gans RO, Wolffenbuttel BH. Thyroid function is associated with components of the metabolic syndrome in euthyroid subjects. J Clin Endocrinol Metab 2007; 92(2): 491-6.
[http://dx.doi.org/10.1210/jc.2006-1718] [PMID: 17090642]
[6]
Mullur R, Liu YY, Brent GA. Thyroid hormone regulation of metabolism. Physiol Rev 2014; 94(2): 355-82.
[http://dx.doi.org/10.1152/physrev.00030.2013] [PMID: 24692351]
[7]
Verga Falzacappa C, Mangialardo C, Madaro L, et al. Thyroid hormone T3 counteracts STZ induced diabetes in mouse. PLoS One 2011; 6(5)e19839
[http://dx.doi.org/10.1371/journal.pone.0019839] [PMID: 21637761]
[8]
Torrance CJ, Devente JE, Jones JP, Dohm GL. Effects of thyroid hormone on GLUT4 glucose transporter gene expression and NIDDM in rats. Endocrinology 1997; 138(3): 1204-14.
[http://dx.doi.org/10.1210/endo.138.3.4981] [PMID: 9048628]
[9]
Gobatto CA, de Mello MA, Sibuya CY, de Azevedo JR, dos Santos LA, Kokubun E. Maximal lactate steady state in rats submitted to swimming exercise. Comp Biochem Physiol A Mol Integr Physiol 2001; 130(1): 21-7.
[http://dx.doi.org/10.1016/S1095-6433(01)00362-2] [PMID: 11672680]
[10]
Sundler F, Grunditz T, Håkanson R, Uddman R. Innervation of the thyroid. A study of the rat using retrograde tracing and immunocytochemistry. Acta Histochem Suppl 1989; 37: 191-8.
[PMID: 2475884]
[11]
Gereben B, Zavacki AM, Ribich S, et al. Cellular and molecular basis of deiodinase-regulated thyroid hormone signaling. Endocr Rev 2008; 29(7): 898-938.
[http://dx.doi.org/10.1210/er.2008-0019] [PMID: 18815314]
[12]
Ribeiro MO, Carvalho SD, Schultz JJ, et al. Thyroid hormone--sympathetic interaction and adaptive thermogenesis are thyroid hormone receptor isoform--specific. J Clin Invest 2001; 108(1): 97-105.
[http://dx.doi.org/10.1172/JCI200112584] [PMID: 11435461]
[13]
Wedick NM, Brennan AM, Sun Q, Hu FB, Mantzoros CS, van Dam RM. Effects of caffeinated and decaffeinated coffee on biological risk factors for type 2 diabetes: a randomized controlled trial. Nutr J 2011; 10: 93.
[http://dx.doi.org/10.1186/1475-2891-10-93] [PMID: 21914162]


Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 16
ISSUE: 3
Year: 2020
Page: [364 - 367]
Pages: 4
DOI: 10.2174/1573401315666181211144036
Price: $65

Article Metrics

PDF: 11
HTML: 2