Simultaneous Determination of Three Alkaloids in Wutou Decoction in Rat Plasma Via the UPLC–MS/MS Method and its Application in Pharmacokinetic Study

Author(s): WU Jian*, JI Yu-bin, Liu Ying-jie, XU Ying, Zheng Wei, Yu Miao

Journal Name: Current Pharmaceutical Analysis

Volume 16 , Issue 5 , 2020


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Graphical Abstract:


Abstract:

Introduction: Wutou decoction has been wildly applied for the treatment of RA in China for several thousand years.

Methods: This study aims to develop a highly sensitive and specific ultra performance liquid chromatography coupled with tandem mass spectrometry and electrospray ionization (UPLC-ESI-MS/MS) method to explore the pharmacokinetic properties of three representative bioactive alkaloids after intragastric administration of Wutou decoction in rats. Chromatographic separation was performed on a C18 column under the Multiple Reaction Monitoring (MRM) in the positive electrospray ionization (ESI) mode. The pharmacokinetic parameters were evaluated by software DAS 3. 0.

Results: The validation of the method was achieved in accordance with the FDA guidelines. The results of pharmacokinetic study showed that the in vivo concentrations of benzoylmesaconine and benzoylhypaconine were significantly higher than benzoylaconine. Our PK results showed that these three compounds were quickly absorbed after the administration of Wutou decoction, and Tmax ranged from 30 min to 45 min.

Conclusion: The results of pharmacokinetic study showed that the in vivo concentrations of benzoylmesaconine and benzoylhypaconine were significantly higher than benzoylaconine. There were also similar pharmacokinetic behaviors observed among BAC, BHA, and BMA after oral administration of WTD.

Keywords: Pharmacokinetic, benzoylhypaconine, benzoylmesaconine, benzoylaconine, Wutou decoction, UPLC–MS/MS.

[1]
Buriani, A.; Garcia-Bermejo, M.L.; Bosisio, E.; Xu, Q.; Li, H.; Dong, X.; Simmonds, M.S.; Carrara, M.; Tejedor, N.; Lucio-Cazana, J.; Hylands, P.J. Omic techniques in systems biology approaches to traditional Chinese medicine research: present and future. J. Ethnopharmacol., 2012, 140(3), 535-544.
[http://dx.doi.org/10.1016/j.jep.2012.01.055] [PMID: 22342380]
[2]
Dong, S.L.; Zhang, L.X. Wu tou decoction in treating 146 cases of rheumatoid arthritis. Zhejiang J Trad Chin Med., 1992, 27, 471-472.
[3]
Li, J.Y. Modified wutou decoction in treating 54 cases of sciatica. Henan Trad Chin Med., 2009, 29, 1055-1056.
[4]
Wang, P. Wu tou decoction in the treatment of scapulohumeral periarthritis. Shanxi J Trad Chin Med., 2001, 22, 629-630.
[5]
Li, M.; He, J.; Jiang, L.L.; Ng, E.S.; Wang, H.; Lam, F.F.; Zhang, Y.M.; Tan, N.H.; Shaw, P.C. The anti-arthritic effects of Aconitum vilmorinianum, a folk herbal medicine in Southwestern China. J. Ethnopharmacol., 2013, 147(1), 122-127.
[http://dx.doi.org/10.1016/j.jep.2013.02.018] [PMID: 23439029]
[6]
Kubo, M.; Moriura, T.; Matsuda, H. [Pharmacological study on Aconiti Tuber. I. Effect of water extract from Aconiti Tuber on adjuvant-induced arthritis]. Yakugaku Zasshi, 1990, 110(1), 16-26.
[http://dx.doi.org/10.1248/yakushi1947.110.1_16] [PMID: 1693957]
[7]
Xue, L.; Zhang, H.Y.; Qin, L.; Wang, X.C.; Wang, L. [Effect of chuanwu and baishao used separately or in combination on adjuvant arthritis in rats]. Zhongguo Zhongyao Zazhi, 2000, 25(3), 175-178.
[PMID: 12212105]
[8]
Zhao, F.C.; Fu, L.; Wu, C.; Lu, J.; Liu, J.; Li, J.; Jiang, L. [Research on the effect of Aconitum soongaricum and its processed products on AA rat]. Zhong Yao Cai, 2012, 35(10), 1572-1576.
[PMID: 23627118]
[9]
Van Landeghem, A.A.; De Letter, E.A.; Lambert, W.E.; Van Peteghem, C.H.; Piette, M.H. Aconitine involvement in an unusual homicide case. Int. J. Legal Med., 2007, 121(3), 214-219.
[http://dx.doi.org/10.1007/s00414-006-0119-5] [PMID: 17021898]
[10]
Singh, S.; Fadnis, P.P.; Sharma, B.K. Aconite poisoning. J. Assoc. Physicians India, 1986, 34(11), 825-826.
[PMID: 2435703]
[11]
Bisset, N. G Arrow poisons in China. Part II. Aconitum-Botany, chemistry, and pharmacology. J. Thnopharmacol., 1981, 6, 247-336.
[12]
Ye, L.; Yang, X.; Yang, Z.; Gao, S.; Yin, T.; Liu, W.; Wang, F.; Hu, M.; Liu, Z. The role of efflux transporters on the transport of highly toxic aconitine, mesaconitine, hypaconitine, and their hydrolysates, as determined in cultured Caco-2 and transfected MDCKII cells. Toxicol. Lett., 2013, 216(2-3), 86-99.
[http://dx.doi.org/10.1016/j.toxlet.2012.11.011] [PMID: 23200901]
[13]
Tang, L.; Gong, Y.; Lv, C.; Ye, L.; Liu, L.; Liu, Z. Pharmacokinetics of aconitine as the targeted marker of Fuzi (Aconitum carmichaeli) following single and multiple oral administrations of Fuzi extracts in rat by UPLC/MS/MS. J. Ethnopharmacol., 2012, 141(2), 736-741.
[http://dx.doi.org/10.1016/j.jep.2011.08.070] [PMID: 21924342]


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Article Details

VOLUME: 16
ISSUE: 5
Year: 2020
Published on: 15 June, 2020
Page: [558 - 563]
Pages: 6
DOI: 10.2174/1573412915666181127151626
Price: $65

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