Title:Repurposing of Existing Drugs for the Bacterial Infections: An In silico and In vitro Study
VOLUME: 20 ISSUE: 2
Author(s):Yachika Kapoor, Ruchika Sharma and Anoop Kumar*
Affiliation:Department of Biotechnology, ISF Institute of Professional Studies (ISFIPS), Moga, Punjab, Department of Biotechnology, ISF Institute of Professional Studies (ISFIPS), Moga, Punjab, Department of Pharmacology, ISF College of Pharmacy (ISFCP), Moga, Punjab
Keywords:Infectious diseases, drug repurposition, In silico, In vitro studies, bacterial infections, metformin, propranolol.
Abstract:
Background: The emergence of infectious diseases and its resistance to approved drugs
is a global threat to human health. The incidence of infectious diseases is increasing day by day
and expected to rise exponentially in next 2 decades. Thus, there is a need for new treatment
modalities for the treatment of microbial infections.
Methods: Thus, in the current investigation, we have explored the existing drugs [metformin
(Anti-diabetic), propranolol (Anti-hypertensive) and amitriptyline (antidepressant)] for treatment
of infectious diseases.
Results: An in-silico result of current investigation has shown the good interaction of metformin,
propranolol, and amitriptyline towards various targets (Beta-lactamase, Penicillin-binding
proteins, Staphylokinase protein, Oxidoreductase protein, etc.) of gram positive and gram negative
bacteria as that of internal ligand. Further, in-vitro results have shown that the antibacterial
activity of metformin, propranolol, and amitriptyline against Bacillus pumilus, Pseudomonas
aeruginosa and Staphylococcus aureus.
Conclusion: The parameters such as Microtiter assay: percentage growth retardation and bacterial
growth kinetics, Minimum inhibitory concentration (MIC), Post-antibiotic assay and Biofilm
formation have indicated the antibacterial potential of metformin, propranolol, and amitriptyline.
However, before starting a clinical trial, complete safety and efficacy profile of drugs in the
treatment of infectious diseases should be investigated.
