Quantitative Determination of Ginsenoside Rg1 in Rat Plasma by Ultrahigh Performance Liquid Chromatography-tandem Mass Spectrometry (UHPLC-MS/MS) and its Application in a Pharmacokinetics and Bioavailability Study

Author(s): Qinghua Weng, Zhenan Zhang, Linglong Chen, Weiwei You, Jinlai Liu, Feifei Li, Lianguo Chen*, Xiajuan Jiang*.

Journal Name: Current Pharmaceutical Analysis

Volume 16 , Issue 1 , 2020

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Graphical Abstract:


Background: Ginsenoside Rg1 (Rg1) is the main active compound of ginseng herbs.

Objective: The aim of this study is to develop a rapid, selective and sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to determine the levels of Rg1 in rat plasma and investigate the pharmacokinetics and bioavailability of Rg1 in rats.

Methods: Chromatographic separation was achieved on an UHPLC-MS/MS system with an UPLC BEH C18 column using an elution gradient of a mixture of acetonitrile and water (with 0.1% formic acid). The analytes were quantitatively determined by negative-mode electrospray tandem MS.

Results: The linearity of the calibration curve was from 2 to 1,000 ng/mL (r ≥ 0.9956), and the lower limit of quantification was 2 ng/mL. The inter-day and intra-day precision were both lower than 12.0%, and the accuracy ranged from 90.6 to 109.7%. The recovery of the targets was higher than 87.0%, and the matrix effect at three different analyte concentrations were from 89.0 to 97.2%. The bioavailability of Rg1 was only 6.1% due to a poor oral absorption.

Conclusion: This new quantitative method was found to be sensitive, rapid and selective, and was successfully used to study the pharmacokinetics of Rg1 after intravenous and oral administration in rats.

Keywords: Ginsenoside Rg1, pharmacokinetics, bioavailability, rat, plasma, UHPLC-MS/MS.

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Year: 2020
Page: [85 - 91]
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DOI: 10.2174/1573412915666181109092728
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