Simultaneous Determination of 17 Constituents of Chinese Wild Radix Salvia miltiorrhiza from Different Geographical Areas by Ultra-High Performance Liquid Chromatography Coupled to Triple Quadrupole Mass Spectrometry

Author(s): Xiao-Dan Zhang, Ye-Sheng Cen, Yan-Ge Yu, Zhe-Chen Qi, Dong-Feng Yang, Zi-Ying Wang, Zhuo-Ni Hou, Zong-Suo Liang*

Journal Name: Current Pharmaceutical Analysis

Volume 16 , Issue 3 , 2020


DOI : 10.2174/1573412914666181105144418

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Graphical Abstract:


Abstract:

Background and Objective: Radix Salvia miltiorrhiza (RSM) has been used clinically for the prevention and treatment of cardiovascular diseases; therefore, it is important to strengthen its quality management. Considering multiple constituents when assessing RSM quality is essential. We established a simple, rapid method to identify and quantify the major bioactive constituents in RSM using ultra high performance liquid chromatography (UPLC) coupled to a triple quadruple mass spectrometry (QqQ-MS).

Methods: We analyzed 17 markers from 50 batches of wild S. miltiorrhiza samples that were collected from different locations in China. The ultrasonic extracts of all samples were determined using the UPLC-QqQ-MS method and were assessed by hierarchical cluster analysis (HCA).

Results: We used this method to analyze 50 sample batches of the 17 compounds and obtained results with excellent linearity (R2, 0.9915-0.9997), precision (relative standard deviation, RSD, 0.15-1.94%), repeatability (RSD, 1.28-4.71%), stability (RSD, 0.97-5.60%) and recovery (RSD, 0.305-6.40%). The hierarchical cluster analysis was used to classify the 50 samples based on the characteristics of the 17 compound markers.

Conclusion: We demonstrated that the developed method was simple, reproducible and sensitive, and it is capable of systematic and scientific evaluation for quality control of RSM. The HCA clearly demonstated that the RSM samples from different locations were significantly different and the quality of wild Radix S. miltiorrhiza could generally be judged according to its geographical origin.

Keywords: UPLC-QqQ-MS, wild Salvia miltiorrhiza, hierarchical cluster analysis, geographical, triple quadrupole, mass spectrometry.

[1]
Lin, Y.; Xu, W.; Huang, M.; Xu, W.; Li, H.; Ye, M.; Zhang, X.; Chu, K. Qualitative and quantitative analysis of phenolic acids, flavonoids and iridoid glycosides in yinhua kanggan tablet by UPLC-QqQ-MS/MS. Molecules, 2015, 20(7), 12209-12228.
[http://dx.doi.org/10.3390/molecules200712209] [PMID: 26151117]
[2]
Slusarczyk, S.; Topolski, J.; Domaradzki, K.; Adams, M.; Hamburger, M.; Matkowski, A. Isolation and fast selective determination of nor-abietanoid diterpenoids from perovskia atriplicifolia roots using LC-ESI-MS/MS with multiple reaction monitoring. Nat. Prod. Commun., 2015, 10(7), 1149-1152.
[http://dx.doi.org/10.1177/1934578X1501000703] [PMID: 26410997]
[3]
Zheng, L.; Gong, Z.; Lu, Y.; Xie, Y.; Huang, Y.; Liu, Y.; Lan, Y.; Wang, A.; Wang, Y.A. UPLC-MS/MS method for simultaneous determination of danshensu, protocatechuic aldehyde, rosmarinic acid, and ligustrazine in rat plasma, and its application to pharmacokinetic studies of Shenxiong glucose injection in rats. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2015, 997, 210-217.
[http://dx.doi.org/10.1016/j.jchromb.2015.06.008] [PMID: 26118621]
[4]
Su, C.Y.; Ming, Q.L.; Rahman, K.; Han, T.; Qin, L.P. Salvia miltiorrhiza: Traditional medicinal uses, chemistry, and pharmacology. Chin. J. Nat. Med., 2015, 13(3), 163-182.
[http://dx.doi.org/10.1016/S1875-5364(15)30002-9] [PMID: 25835361]
[5]
Liu, A.H.; Lin, Y.H.; Yang, M.; Guo, H.; Guan, S.H.; Sun, J.H.; Guo, D.A. Development of the fingerprints for the quality of the roots of Salvia miltiorrhiza and its related preparations by HPLC-DAD and LC-MS(n). J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2007, 846(1-2), 32-41.
[http://dx.doi.org/10.1016/j.jchromb.2006.08.002] [PMID: 16962399]
[6]
Jia, Y.; Huang, F.; Zhang, S.; Leung, S.W. Is danshen (Salvia miltiorrhiza) dripping pill more effective than isosorbide dinitrate in treating angina pectoris? A systematic review of randomized controlled trials. Int. J. Cardiol., 2012, 157(3), 330-340.
[http://dx.doi.org/10.1016/j.ijcard.2010.12.073] [PMID: 21251721]
[7]
Qin, J.; Jiang, L. Effect of Danshen injections on NT-proBNP in patients with hypertension and left ventricular failure. Int. J. Lab. Med., 2016, 19, 2728-2729.
[8]
Zhou, L.; Chow, M.; Li, C.L.; Si, Q.J. A Treatment study of danhong injection (Herbal TCM product from Salviae Miltiorrhizae and flos carthami tinctorii) on the elderly unstable angina patients. J. Am. Geriatr. Soc., 2015, S375-S376.
[9]
Zuo, Z. Improved quality control method for danshen products-consideration of both hydrophilic and lipophilic active components. J. Pharm. Biomed. Anal., 2006, 3, 744-750.
[10]
Zhang, X.; Yu, Y.; Cen, Y.; Yang, D.; Qi, Z.; Hou, Z.; Han, S.; Cai, Z.; Liu, K. Bivariate correlation analysis of the chemometric profiles of chinese wild Salvia miltiorrhiza based on UPLC-Qqq-MS and antioxidant activities. Molecules, 2018, 23(3), 538.
[http://dx.doi.org/10.3390/molecules23030538] [PMID: 29495564]
[11]
Zhang, X.D.; Yu, Y.G.; Yang, D.F.; Qi, Z.C.; Liu, R.Z.; Deng, F.T.; Cai, Z.X.; Li, Y.; Sun, Y.F.; Liang, Z.S. Chemotaxonomic variation in secondary metabolites contents and their correlation between environmental factors in Salvia miltiorrhiza Bunge from natural habitat of China. Ind. Crops Prod., 2018, 113, 335-347.
[http://dx.doi.org/10.1016/j.indcrop.2018.01.043]
[12]
Cao, J.; Wei, Y.J.; Qi, L.W.; Li, P.; Qian, Z.M.; Luo, H.W.; Chen, J.; Zhao, J. Determination of fifteen bioactive components in Radix et Rhizoma Salviae Miltiorrhizae by high-performance liquid chromatography with ultraviolet and mass spectrometric detection. Biomed. Chromatogr., 2008, 22(2), 164-172.
[http://dx.doi.org/10.1002/bmc.911] [PMID: 17853389]
[13]
Zhong, G.X.; Li, P.; Zeng, L.J.; Guan, J.; Li, D.Q.; Li, S.P. Chemical characteristics of Salvia miltiorrhiza (Danshen) collected from different locations in China. J. Agric. Food Chem., 2009, 57(15), 6879-6887.
[http://dx.doi.org/10.1021/jf901462s] [PMID: 19601629]
[14]
Lin, L.; Ni, B.; Lin, H.; Zhang, M.; Yan, L.; Qu, C.; Ni, J. Simultaneous determination of 14 constituents of Radix polygoni multiflori from different geographical areas by liquid chromatography-tandem mass spectrometry. Biomed. Chromatogr., 2015, 29(7), 1048-1055.
[http://dx.doi.org/10.1002/bmc.3391] [PMID: 2545050]
[15]
Hu, P.; Liang, Q.L.; Luo, G.A.; Zhao, Z.Z.; Jiang, Z.H. Multi-component HPLC fingerprinting of Radix Salviae Miltiorrhizae and its LC-MS-MS identification. Chem. Pharm. Bull. (Tokyo), 2005, 53(6), 677-683.
[http://dx.doi.org/10.1248/cpb.53.677] [PMID: 15930782]
[16]
Chinese Pharmcopoeia Commision. Pharmacopoeia of the people’s Republic of China; China Medical Science Press: Beijing, 2015, pp. 76-77.
[17]
Zhang, C.L.; Liang, Z.S.; Guo, H.B.; Liu, J.L.; Liu, Y.; Liu, F.H.; Wei, L.Z. [Correlation analysis between meteorological factors, biomass, and active components of Salvia miltiorrhiza in different climatic zones]. Zhongguo Zhongyao Zazhi, 2015, 40(4), 607-613.
[PMID: 26137677]
[18]
Tokalıoğlu, Ş. Determination of trace elements in commonly consumed medicinal herbs by ICP-MS and multivariate analysis. Food Chem., 2012, 134(4), 2504-2508.
[http://dx.doi.org/10.1016/j.foodchem.2012.04.093] [PMID: 23442717]


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Article Details

VOLUME: 16
ISSUE: 3
Year: 2020
Published on: 30 March, 2020
Page: [280 - 290]
Pages: 11
DOI: 10.2174/1573412914666181105144418
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